Construction of in situ modulated controlled growth of MOF-on-mof impedimetric assembly for the practical minimal level assessment of anti-mullerian hormone.

Anti-mullerian hormone (AMH) detection receives much attention since it is used as an ideal biomarker for quantitative assessment of ovarian reserve. The present study proposed a first report on the use of MOF-on-MOF as an electrochemical sensor for recognizing AMH in buffer and serum media. The MOF-on-MOF, MIL-88 B@UiO66NH2 was synthesized by the internal extended growth method (IEGM) involving MIL-88 B on UiO66NH2 by in situ method for the first time. MOF matrix could be established to form a three-dimensional (3D) core-shell hybrid unit using MOFs with distinct characteristics. The morphology, structural characteristics, and electrochemical performance of MIL-88 B@UiO66NH2 were studied. It was successfully used for AMH sensing to demonstrate the detection performance of the internal extended growth method (IEGM) grown MIL-88 B@UiO66NH2 made immunosensor. The electrochemical results indicated that MOF-on-MOF exhibited linear EIS response for AMH concentration varying from 100 ng/mL to 1 fg/mL. Further, the immunosensor displayed high specificity and sensitivity for AMH detection. The fabricated sensor attained a remarkable limit of detection (LOD) of 1.07 fg/mL and 0.82 fg/mL, when studied in PBS and 10% serum buffer media, respectively. The biosensor achieved the limit of quantification (LOQ) of 3.25 fg/mL and 2.5 fg/mL, respectively, when analyzed in PBS buffer and 10% serum buffer. The significant results emphasized that the fabricated biosensor holds a promising potential to act as an appropriate tool for rapid assessment of AMH levels.

Fang WZ ，Palanisamy S ，Vijayaraghavan P ，Chen CY ，Weng SL ，Yang CS ，Wang YM ... -  《-》

Electrochemiluminescence immunosensor using a lanthanum-based metal-organic framework as signal probe and Cu(2)MoS(4) as a co-reaction promoter for the sensitive detection of anti-Müllerian hormone.

In this study, we used meso-tetra (4-carboxyphenyl) porphyrin (TCPP) as an organic ligand to modify a lanthanide-based metal-organic framework as an electrochemiluminescence (ECL) platform to sensitively detect anti-Müllerian hormone (AMH). La-MOF amplified the ECL signal by suppressing the aggregation-caused quenching created by TCPP self-aggregation. Utilizing the reversible cycling of the mixed-valence transition metal ion (Cu+/Cu2+ and Mo4+/Mo6+) and the electrical conductivity of Cu2MoS4 and silver nanoparticle (AgNP), Cu2MoS4-AgNP as a dual co-reaction promoter constantly generated sulfate radical anions (SO4•-) and thus amplified the ECL signal. Based on this strategy, we built a sandwich-type ECL immunosensor for the sensitive detection of AMH with a linear range of 1 × 10-4 to 50 ng/mL and a limit of detection of 24 fg/mL. This study provides a novel approach for the sensitive and selective detection of AMH, demonstrating its high potential and practical value for the sensitive detection of AMH.

Li J ，Hu S ，Zheng L ，Xu L ，Wu Y ，Deng B ... -  《-》

Label-Free Bimetallic In Situ-Grown 3D Nickel-Foam-Supported NH(2)-MIL-88B(Fe(2)Co)-MOF-based Impedimetric Immunosensor for the Detection of Cardiac Troponin I.

Palanisamy S ，Senthil Raja D ，Subramani B ，Wu TH ，Wang YM ... -  《-》

Label-Free Electrochemical Immunosensor for Ultrasensitive Detection of Carbohydrate Antigen 125 Based on Antibody-Immobilized Biocompatible MOF-808/CNT.

Biswas S ，Lan Q ，Xie Y ，Sun X ，Wang Y ... -  《-》

Sensitive sandwich-type voltammetric immunosensor for breast cancer biomarker HER2 detection based on gold nanoparticles decorated Cu-MOF and Cu(2)ZnSnS(4) NPs/Pt/g-C(3)N(4) composite.

Yola ML 《-》