Electrochemiluminescence immunosensor using a lanthanum-based metal-organic framework as signal probe and Cu(2)MoS(4) as a co-reaction promoter for the sensitive detection of anti-Müllerian hormone.

In this study, we used meso-tetra (4-carboxyphenyl) porphyrin (TCPP) as an organic ligand to modify a lanthanide-based metal-organic framework as an electrochemiluminescence (ECL) platform to sensitively detect anti-Müllerian hormone (AMH). La-MOF amplified the ECL signal by suppressing the aggregation-caused quenching created by TCPP self-aggregation. Utilizing the reversible cycling of the mixed-valence transition metal ion (Cu+/Cu2+ and Mo4+/Mo6+) and the electrical conductivity of Cu2MoS4 and silver nanoparticle (AgNP), Cu2MoS4-AgNP as a dual co-reaction promoter constantly generated sulfate radical anions (SO4•-) and thus amplified the ECL signal. Based on this strategy, we built a sandwich-type ECL immunosensor for the sensitive detection of AMH with a linear range of 1 × 10-4 to 50 ng/mL and a limit of detection of 24 fg/mL. This study provides a novel approach for the sensitive and selective detection of AMH, demonstrating its high potential and practical value for the sensitive detection of AMH.

Li J ，Hu S ，Zheng L ，Xu L ，Wu Y ，Deng B ... -  《-》

An electrochemiluminescence biosensor based on silver-cysteine nanorod as an emitter and AgNP-decorated FeMoO(ν) as a signal amplifier for sensitive detection of heart-type fatty acid binding protein.

An electrochemiluminescence (ECL) immunosensor was developed for the highly sensitive and specific detection of heart-type fatty acid binding protein (H-FABP) and the rapid diagnosis of acute myocardial infarction (AMI). H-FABP is a biomarker that is highly specific to cardiac tissue and is associated with a range of cardiac diseases. Following myocardial injury, the rate of increase in H-FABP levels is greater than that observed for myoglobin and troponin. Therefore, the measurement of H-FABP is crucial for the early exclusion of AMI. Silver-cysteine nanorod (AgCysNR), which served as the ECL emitter, was produced with a one-step, green, simple, template-free aqueous phase method. The surfaces of AgCysNR displayed many amino and carboxyl groups that were connected to a large number of a secondary H-FABP-specific antibody. Ferrum-doped molybdenum oxide (FeMoOν), with a large specific surface area, was richly decorated with silver nanoparticle (AgNP), which increased the interfacial electron transfer rate of FeMoOν. The AgNP was used as a co-reaction accelerator to promote persulfate to produce more sulfate anion radical and then enhance the ECL intensity of AgCysNR. The linear range of the ECL immunosensor was 10 fg/mL to 100 ng/mL, and the detection limit was 2.3 fg/mL (signal/noise = 3). The sensor was determined to be stable, repeatable, and reproducible, and the method achieved recoveries of 101.0 to 102.6% with relative standard deviations of 1.4 to 2.0%. This immunosensor represents a promising tool for the early diagnosis of AMI.

Zheng L ，Li J ，Hu S ，Xu L ，Wu Y ，Deng B ... -  《-》

Electrochemiluminescence immunosensor based on a novel heterostructured Fe-MIL-88@1T-MoS(2) dual-nanozyme with high peroxidase-like activity for the sensitive detection of NT-proBNP.

Jiang X ，Su W ，Shi W ，Wang H ... -  《-》

A sensitive electrochemiluminescence immunosensor for CEA detection based on the ECL-RET between zinc-based metal-organic frameworks and ZiF-8@PDA.

In this study, we developed a new system that using zinc-based metal-organic frameworks NH2-Zn-PTC as the donor and ZiF-8@PDA as the acceptor to achieve highly sensitive detection of carcinoembryonic antigen (CEA), using the fundamentals of electrochemiluminescence resonance energy transfer (ECL-RET). Firstly, the aggregation-induced quenching effect (ACQ) was eliminated by the coordination of PTC in MOF and the ECL signal was improved. Secondly, the ECL signal was further amplified by using Au NPs and amino groups as co-reaction promoters to generate more SO4.-. In addition, the introduction of ZiF-8@PDA as an acceptor and NH2-Zn-PTC as a donor took advantage of the feature of partial overlap of the UV-vis absorption spectrum and ECL emission spectra between the two, thereby effectively initiating the ECL-RET behavior, which improved the detection sensitivity of the sensor. The prepared immunosensor showed good linearity in the concentration range of 10-4 to 80 ng/mL with a detection limit of 18.20 fg/mL. This makes it promising for clinical testing of tumor markers.

Li Y ，Cheng Y ，Ren H ，Ji T ，Zhao Z ，Li H ，Hong C ... -  《-》

Construction of in situ modulated controlled growth of MOF-on-mof impedimetric assembly for the practical minimal level assessment of anti-mullerian hormone.

Anti-mullerian hormone (AMH) detection receives much attention since it is used as an ideal biomarker for quantitative assessment of ovarian reserve. The present study proposed a first report on the use of MOF-on-MOF as an electrochemical sensor for recognizing AMH in buffer and serum media. The MOF-on-MOF, MIL-88 B@UiO66NH2 was synthesized by the internal extended growth method (IEGM) involving MIL-88 B on UiO66NH2 by in situ method for the first time. MOF matrix could be established to form a three-dimensional (3D) core-shell hybrid unit using MOFs with distinct characteristics. The morphology, structural characteristics, and electrochemical performance of MIL-88 B@UiO66NH2 were studied. It was successfully used for AMH sensing to demonstrate the detection performance of the internal extended growth method (IEGM) grown MIL-88 B@UiO66NH2 made immunosensor. The electrochemical results indicated that MOF-on-MOF exhibited linear EIS response for AMH concentration varying from 100 ng/mL to 1 fg/mL. Further, the immunosensor displayed high specificity and sensitivity for AMH detection. The fabricated sensor attained a remarkable limit of detection (LOD) of 1.07 fg/mL and 0.82 fg/mL, when studied in PBS and 10% serum buffer media, respectively. The biosensor achieved the limit of quantification (LOQ) of 3.25 fg/mL and 2.5 fg/mL, respectively, when analyzed in PBS buffer and 10% serum buffer. The significant results emphasized that the fabricated biosensor holds a promising potential to act as an appropriate tool for rapid assessment of AMH levels.

Fang WZ ，Palanisamy S ，Vijayaraghavan P ，Chen CY ，Weng SL ，Yang CS ，Wang YM ... -  《-》