Zinc fertilisation increases grain zinc and reduces grain lead and cadmium concentrations more in zinc-biofortified than standard wheat cultivar.

Given that plant uptake and transport systems for metals have some similarities, zinc (Zn)-biofortified cultivars may concurrently accumulate non-essential toxic heavy metals in grains. However, Zn-biofortified cultivars have never been tested for heavy metal accumulation in grains. In a pot experiment, we compared Zn-biofortified wheat (Zincol-2016) with a standard wheat (Faisalabad-2008) cultivar on heavy-metal-contaminated soils for yield response and grain accumulation of Zn, lead (Pb) and cadmium (Cd), without or with Zn fertilisation (8mgZnkg-1). The soils, collected from agricultural fields in (i) industrial zone and (ii) peri-urban area, had been receiving industrial and city effluents for >20years. In the two soils, Zn fertilisation significantly (P≤0.05) increased grain yield of both cultivars. Zinc fertilisation increased grain Zn concentration of Zincol-2016 and Faisalabad-2008 by respectively 32 and 18% in industrial-zone soil, and by 15 and 2% in peri-urban soil. Averaged across Zn rates, Zincol-2016 accumulated in grains more than double the Zn amount than Faisalabad-2008 in industrial-zone soil. At 0mgZnkg-1, grain Pb and Cd concentrations were respectively 26 and 33% greater in Zincol-2016 than Faisalabad-2008 in industrial-zone soil, and 86 and 50% greater in Zincol-2016 than Faisalabad-2008 in peri-urban soil. Zinc fertilisation significantly (P≤0.05) decreased concentration of Pb and Cd in grains of both cultivars. In industrial-zone soil, a toxic level of Pb in grains (0.24mgkg-1) was attained at control rate of Zn by Zincol-2016, and was decreased to a safe level (0.07mgkg-1) by application of 8mgZnkg-1. Therefore, biofortified cultivars should not be grown in contaminated soils, and/or sufficient Zn must be applied, to decrease accumulation of non-essential toxic heavy metals in grains. Moreover, future breeding efforts should be directed toward selection of biofortified cultivars that would selectively accumulate Zn in grains, but not the contaminants.

Qaswar M ，Hussain S ，Rengel Z 《-》

Zinc-biofortified wheat accumulates more cadmium in grains than standard wheat when grown on cadmium-contaminated soil regardless of soil and foliar zinc application.

Zinc (Zn)-biofortified wheat may contribute to decreasing widespread human Zn deficiency. Such genotypes may also accumulate cadmium (Cd) in grains that would expect to be decreased by Zn application. However, the influence of soil and foliar Zn application on grain Cd accumulation in Zn-biofortified versus standard wheat is unknown. In our experiment, we grew standard (Faisalabad-2008) and Zn-biofortified (Zincol-2016) wheats in pots having uncontaminated (T0) or Cd-spiked (8 mg kg-1) soil. Plants in Cd-amended pots were treated with no Zn (T1), 8 mg Zn kg-1 to soil at sowing (T2), 0.5% w/v ZnSO4·7H2O to foliage at booting and heading (T3), or soil (as in T2) + foliar (as in T3) Zn application (T4). Only in the uncontaminated control, grain yield of Faisalabad-2008 was greater than Zincol-2016. Any Zn application to Zincol-2016 grown in Cd-spiked pots increased grain yield compared with the uncontaminated control. In both cultivars, grain Zn concentration was influenced more by foliar than soil Zn application. However, Zincol-2016 had 6 to 14 mg more Zn kg-1 in grains than Faisalabad-2008 in the comparable treatments. Cadmium exposure (T1 vs. T0) decreased grain yield of only Faisalabad-2008, and decreased grain Zn concentration only in Zincol-2016. Without any Zn application, grain Cd concentration in both cultivars exposed to Cd was above the permissible level (0.20 mg kg-1). Zinc application decreased grain Cd concentration, although it remained above the permissible level in both cultivars except in Faisalabad-2008 when treated with soil + foliar Zn. Foliar Zn application decreased grain Cd concentration more than soil Zn application, and more in Zincol-2016 than Faisalabad-2008. In the comparable Cd-spiked treatments, Zincol-2016 had 73 to 134% higher grain Cd concentration than Faisalabad-2008. The Zn-biofortified genotypes accumulating toxic metals may pose serious health issues. Therefore, future breeding for biofortification should focus on the selective accumulation of Zn.

Hussain S ，Khan AM ，Rengel Z 《-》

Screening stably low cadmium and moderately high micronutrients wheat cultivars under three different agricultural environments of China.

Heavy metal-contaminated farmland, especially for cadmium (Cd), is being used for agricultural production in large scale due to the increasing food demand. Thus, minimizing the influx of Cd to the human food chain is urgently needed. Screening for stably low Cd and moderately high micronutrient wheat cultivars is one of the most feasible and effective approaches to ensure food safety and quality. Here, the Cd accumulation by 72 wheat cultivars was identified in field 1, and the stability of Cd accumulation in these cultivars was tested in fields 2 and 3. The effects of Cd on micronutrient (zinc (Zn), copper (Cu), iron (Fe), manganese (Mn), and boron (B)) uptake in grains were also investigated. Nine of the 24 low-Cd wheat cultivars identified by screening showed stably low-Cd and moderately high micronutrient concentrations in grain. Nine cultivars exhibited unstable low-Cd accumulation characteristics, and another five cultivars contained significantly lower Zn concentrations in grain in at least two experimental fields. One low-Cd cultivar also had low Zn, Cu and Mn concentrations in grain. The accumulation of Cd in wheat grain had little effects on the uptake of Fe. Grain Cd concentration correlated positively with the dry weight of stem and root, and negatively with the spike length. There was no correlation between grain Cd concentration and wheat yield. The selected cultivars with stably low-Cd accumulation and moderately high micronutrient concentrations in grain are recommended for cultivation in slightly to moderately Cd-contaminated farmland to ensure food safety for the growing human population.

Liu N ，Huang X ，Sun L ，Li S ，Chen Y ，Cao X ，Wang W ，Dai J ，Rinnan R ... -  《-》

[Blocking Effects of Foliar Conditioners on Cadmium, Arsenic, and Lead Accumulation in Wheat Grain in Compound-contaminated Farmland].

Xiao B ，Wang QS ，Gao PP ，Zhao QL ，Yang W ，Wang Z ，Liu WJ ，Xue PY ... -  《-》

Effects of alkaline and bioorganic amendments on cadmium, lead, zinc, and nutrient accumulation in brown rice and grain yield in acidic paddy fields contaminated with a mixture of heavy metals.

He H ，Tam NF ，Yao A ，Qiu R ，Li WC ，Ye Z ... -  《-》