Daphnane-type diterpenoid glucosides and further constituents of Euphorbia pilosa.

Phytochemical investigation of whole plants of Euphorbia pilosa led to the isolation and identification of two new daphnane-diterpenoid glucosides, euphopilosides A and B (1 and 2, resp.), and a new ent-abietane, euphopilolide (3), together with eight known compounds. Compounds 1 and 2 are the first daphnane-type diterpenoid glycosides. Their structures were elucidated by a combination of 1D- and 2D-NMR, and MS analyses, and acid hydrolysis. Compounds 1-9 were evaluated for their in vitro cytotoxicities against five human tumor cell lines, HL-60, SMMC-7721, A-549, MCF-7, and SW-480. Compound 7 showed moderate inhibitory activity against all five cell lines.

Zhang XD ，Ni W ，Yan H ，Li GT ，Zhong HM ，Li Y ，Liu HY ... -  《-》

Tigliane-type diterpenoid glycosides from Euphorbia fischeriana.

Pan LL ，Fang PL ，Zhang XJ ，Ni W ，Li L ，Yang LM ，Chen CX ，Zheng YT ，Li CT ，Hao XJ ，Liu HY ... -  《-》

Casbane diterpenoids from the roots of Euphorbia pekinensis.

Four casbane diterpenoids, together with three known related compounds were isolated from the roots of Euphorbia pekinensis. Their structures were elucidated on the basis of spectroscopic studies and comparison with the known related compounds. In addition, the absolute configuration of three compounds was determined by the modified Mosher's method. The isolated compounds were evaluated for their cytotoxic activity against seven human cancer cell lines by a WST-8 assay.

Tao WW ，Duan JA ，Tang YP ，Yang NY ，Li JP ，Qian YF ... -  《-》

Two new diterpene derivatives from Euphorbia lunulata Bge and their anti-proliferative activities.

A new ent-abietane-type diterpene lactone (1) and a new jatrophane-type diterpenoid (2), together with twelve known compounds including three diterpenes (3-5), five triterpenes (6-10) and four sterides (11-14) were isolated from the ethanol extract of the whole plant of Euphorbia lunulata Bge. The structure of compounds 1 and 2 was elucidated on the basis of 1D and 2D NMR spectra and the HR-ESI-MS data. The structure of compound 2 was further analyzed by an X-ray crystallographic study. The in vitro anti-proliferative activities against MCF-7 and NCI-H460 cell lines for compounds 1-5 (diterpene) were evaluated. The results showed marked activity for compound 1 against the two cell lines with the IC50 values 19.5 (NCI-H460) and 18.6 (MCF-7) μM, while for cis-platinum (a positive cytotoxic control agent) 29.7 (NCI-H460) and 27.7 (MCF-7) μM. Compounds 2-5 exhibited moderate cytotoxic activities for the two cell lines with the IC50 values ranging from 32.1 to 58.2 μM.

Liu C ，Liao ZX ，Liu SJ ，Qu YB ，Wang HS ... -  《-》

Lathyrane-type diterpenoids from the seeds of Euphorbia lathyris.

Ten lathyrane-type diterpenoids named Euphorbia Factor L12-L21 (1-10) and twelve known diterpenoids (11-22) were isolated from seeds of Euphorbia lathyris. The structures of these compounds were determined by extensive spectroscopic (UV, IR, HRESIMS, 1D and 2D NMR) analyses. In addition, the configuration of Euphorbia Factor L12 (1) was further confirmed by X-ray crystallographic and circular dichroism (CD) analyses. A putative biogenetic relationship to these compounds was proposed. Cytotoxicity of the isolated compounds against C6 and MCF-7 cell lines were evaluated. Compounds 1, 5, 7, 12 and 17 exhibited considerable cytotoxic activities (IC50 12.4-36.2 μM).

Lu J ，Li G ，Huang J ，Zhang C ，Zhang L ，Zhang K ，Li P ，Lin R ，Wang J ... -  《-》