Conserved cross-interactions in Drosophila and Xenopus between Ras/MAPK signaling and the dual-specificity phosphatase MKP3.

The extracellular signal-regulated kinase (ERK) is a key transducer of the epidermal growth factor receptor (EGFR) and fibroblast growth factor receptor (FGFR) signaling pathways, and its function is required in multiple processes during animal development. The activity of ERK depends on the phosphorylation state of conserved threonine and tyrosine residues, and this state is regulated by different kinases and phosphatases. A family of phosphatases with specificity toward both threonine and tyrosine residues in ERK (dual-specificity phosphatases) play a conserved role in its dephosphorylation and consequent inactivation. Here, we characterize the function of the dual-specificity phosphatase MKP3 in Drosophila EGFR and Xenopus FGFR signaling. The function of MKP3 is required during Drosophila wing vein formation and Xenopus anteroposterior neural patterning. We find that the expression of the MKP3 gene is localized in places of high EGFR and FGFR signaling. Furthermore, this restricted expression depends on ERK function both in Drosophila and Xenopus, suggesting that MKP3 constitutes a conserved negative feedback loop on the activity of the Ras/ERK signaling pathway.

Gómez AR ，López-Varea A ，Molnar C ，de la Calle-Mustienes E ，Ruiz-Gómez M ，Gómez-Skarmeta JL ，de Celis JF ... -  《DEVELOPMENTAL DYNAMICS》

EGFR and FGFR signaling through FRS2 is subject to negative feedback control by ERK1/2.

Wu Y ，Chen Z ，Ullrich A 《BIOLOGICAL CHEMISTRY》

A role for MKP3 in axial patterning of the zebrafish embryo.

Tsang M ，Maegawa S ，Kiang A ，Habas R ，Weinberg E ，Dawid IB ... -  《DEVELOPMENT》

Sprouty1 and Sprouty2 provide a control mechanism for the Ras/MAPK signalling pathway.

Hanafusa H ，Torii S ，Yasunaga T ，Nishida E ... -  《NATURE CELL BIOLOGY》

Intramolecular dephosphorylation of ERK by MKP3.

Kim Y ，Rice AE ，Denu JM 《BIOCHEMISTRY》