MTHFR C677T polymorphism and its relation to ischemic stroke in the Black Sea Turkish population.

The MTHFR C677T mutation has been shown to be associated with venous thrombosis. The role of this mutation in ischemic stroke is unclear. We investigated whether the MTHFR mutation is a risk factor for patients with ischemic stroke in the Black Sea Turkish population or not. We analyzed 30 patients (19 male, 11 female) [median age: 50 years (range: 28-78)] with ischemic stroke who had no known predisposition factors for stroke and 242 (182 male, 60 female) healthy controls [median age: 42 years (range: 18-65)]. Detection of the MTHFR C677T mutation was performed by using commercially available allele-specific PCR-ELISA kits. Prevalence of the MTHFR C677T genotype was 49.1% (CT, 45.8%; TT, 3.3%) in controls and 50% (CT, 43.3%; TT, 6.6%) in patients [OR: 1.03, 95% CI (0.45-2.35]). The prevalence of homozygous gene mutation for MTHFR was higher among patients with stroke than control subjects, but this difference was not statistically significant. The MTHFR gene mutation is not a risk factor for ischemic stroke formation in patients from the Black Sea region in Turkey.

Uçar F ，Sönmez M ，Ovali E ，Ozmenoglu M ，Karti SS ，Yilmaz M ，Pakdemir A ... -  《AMERICAN JOURNAL OF HEMATOLOGY》

Methylenetetrahydrofolate reductase C677T polymorphism in Iraqi patients with ischemic stroke.

Al-Allawi NA ，Avo AS ，Jubrael JM 《NEUROLOGY INDIA》

The C677T polymorphism of the methylenetetrahydrofolate reductase gene is associated with idiopathic ischemic stroke in the young Mexican-Mestizo population.

Previous studies have demonstrated that a common polymorphism in the gene encoding 5,10-methylenetetrahydrofolate reductase (MTHFR) is associated with an increased risk for stroke. However, this relation remains controversial. Our aim was to investigate the possible association between the C677T polymorphism in the MTHFR gene and idiopathic ischemic stroke in the young Mexican-Mestizo population. One hundred seventy-eight patients <45 years with idiopathic ischemic stroke and 183 controls were tested for the C677T polymorphism in the MTHFR gene. Causes of primary thrombophilia as well as classical risk factors for atherothrombotic disease were also evaluated. There was a significant difference in the genotype distribution between patients and controls (p = 0.01), but the allele frequency was similar in both groups (p = 0.09). The univariate analysis identified the T allele as a risk factor for ischemic stroke (TT and CT carriers), as compared with homozygous for C allele (p = 0.01). Hypertension and smoking prevalences were significantly higher in the group of patients. Also the T allele was significantly associated with large-vessel ischemic stroke. The postoral methionine load homocysteine levels were higher in patients with ischemic stroke versus controls (p < 0.001). There was a low prevalence of primary thrombophilia markers. The T allele from the C677T polymorphism of the MTHFR gene represents an independent risk factor for idiopathic ischemic stroke at young age in the Mexican-Mestizo population. Also, hypertension and smoking were independent risk factors in our study population. Primary thrombophilic risk factors were not associated with ischemic stroke in our population.

Isordia-Salas I ，Barinagarrementería-Aldatz F ，Leaños-Miranda A ，Borrayo-Sánchez G ，Vela-Ojeda J ，García-Chávez J ，Ibarra-González I ，Majluf-Cruz A ... -  《-》

Evaluation of MTHFR C677T polymorphism in ischemic and hemorrhagic stroke patients. A case-control study in a Northern Indian population.

The present study was aimed to evaluate MTHFR C677T gene polymorphism in patients with ischemic stroke (IS) and intracerebral hemorrhage (ICH) and compare it with controls. 207 patient with IS and 215 with CT/MRI proven ICH were included and compared with 188 healthy controls. The stroke risk factors, location of IS, its vascular territory and in ICH the location of hematoma were noted. MTHFR C677T polymorphism was studied by polymerase chain reaction. Hypertension was present in 65.9% of ICH and 48.8% of IS. Other stroke risk factors were not significantly different. The frequency of the CC genotype in controls was 68.6%. CT in 28.7% and TT in 2.7%, whereas it was 75.3%, 20.5% and 4.2% in ICH and 66.2%, 39.4% and 2.4% respectively in IS. The frequency of these genotypes as well as allele frequency was not different in IS, ICH as compared to controls, however variant allele was more frequent in IS compared to ICH. Homocysteine level was higher in IS patients with variant genotype MTHFR C677T gene polymorphism was neither associated with hemorrhagic nor ischemic stroke. However raised homocysteine levels were found to be associated with MTHFRC677-TT genotype in IS patients.

Somarajan BI ，Kalita J ，Mittal B ，Misra UK ... -  《-》

Prevalence of thrombophilic mutations and ACE I/D polymorphism in Turkish ischemic stroke patients.

Celiker G ，Can U ，Verdi H ，Yazici AC ，Ozbek N ，Atac FB ... -  《CLINICAL AND APPLIED THROMBOSIS-HEMOSTASIS》