Expression of Ol-KIP, a cyclin-dependent kinase inhibitor, in embryonic and adult medaka (Oryzias latipes) central nervous system.

From an expression screen in a fish model, the medaka, we have isolated Ol-KIP (Oryzias latipes-kinase inhibitor protein), a new member of the KIP subfamily of cyclin-dependent kinase (Cdk) inhibitors. We have analysed its expression in the developing and adult brain by in situ hybridization and by double labeling with Ol-KIP mRNA and proliferating cell nuclear antigen (PCNA) antibodies. Ol-KIP presents a complex expression pattern in several areas of the embryonic central nervous system, most often in close vicinity to proliferative neuroepithelia. We studied in great detail its expression in the optic tectum: Ol-KIP is expressed in a ring-shaped domain lying exactly between the proliferative and the postmitotic zones of this structure and is, therefore, potentially involved in cell cycle exit. In the adult CNS, Ol-KIP expression persists in numerous nuclei, both close and distant from proliferative ventricular areas. So, Ol-KIP expression is in part compatible with a sustained "stop signal" role for proliferation, but its expression in postmitotic zones suggests that KIP proteins may have late neuronal function(s), in addition to inhibiting Cdks. This first detailed study of the expression profile of a KIP gene in a nonmammalian vertebrate, thus, opens perspectives for analysing the role of these regulators in brain development and function.

Nguyên V ，Candal Suárez EM ，Sharif A ，Joly JS ，Bourrat F ... -  《DEVELOPMENTAL DYNAMICS》

Expression domains suggest cell-cycle independent roles of growth-arrest molecules in the adult brain of the medaka, Oryzias latipes.

Candal E ，Nguyen V ，Joly JS ，Bourrat F ... -  《BRAIN RESEARCH BULLETIN》

Ol-insm1b, a SNAG family transcription factor involved in cell cycle arrest during medaka development.

Candal E ，Alunni A ，Thermes V ，Jamen F ，Joly JS ，Bourrat F ... -  《DEVELOPMENTAL BIOLOGY》

Nanog regulates proliferation during early fish development.

Camp E ，Sánchez-Sánchez AV ，García-España A ，Desalle R ，Odqvist L ，Enrique O'Connor J ，Mullor JL ... -  《-》

Cip/Kip proteins: more than just CDKs inhibitors.

Denicourt C ，Dowdy SF 《-》