Immunoprotective determinants in friend murine leukemia virus envelope protein.

Several immunological epitopes are known to be located within the Friend murine leukemia virus (F-MuLV) envelope protein, but their relative contributions to protection from Friend virus-induced disease are not known. To determine how expression of various immunological determinants affected protection, mice were immunized with recombinant vaccinia viruses expressing different portions of the F-MuLV envelope protein, and they were then challenged with a lethal dose of Friend virus complex. The disease parameters that were followed in the mice were early viremia, early splenomegaly, and late splenomegaly. Both the N-terminal and C-terminal portions of the F-MuLV gp70 were found to protect against late splenomegaly, the primary clinical sign associated with virus-induced erythroleukemia. However, neither region alone protected against early splenomegaly and early viremia, indicating poor immunological control over early virus replication and spread through the spleen and blood. In contrast, mice immunized with a vaccine expressing the entire F-MuLV envelope protein were protected against all three disease parameters. The results indicated that expression of multiple immunological determinants including both T-helper and B cell epitopes was necessary for full protection.

Hasenkrug KJ ，Brooks DM ，Robertson MN ，Srinivas RV ，Chesebro B ... -  《VIROLOGY》

Immunization with a single T helper cell epitope abrogates Friend virus-induced early erythroid proliferation and prevents late leukemia development.

Miyazawa M ，Fujisawa R ，Ishihara C ，Takei YA ，Shimizu T ，Uenishi H ，Yamagishi H ，Kuribayashi K ... -  《JOURNAL OF IMMUNOLOGY》

Identification of a protective CD4+ T-cell epitope in p15gag of Friend murine leukemia virus and role of the MA protein targeting the plasma membrane in immunogenicity.

Sugahara D ，Tsuji-Kawahara S ，Miyazawa M 《-》

Peptide-induced immune protection of CD8+ T cell-deficient mice against Friend retrovirus-induced disease.

Kawabata H ，Niwa A ，Tsuji-Kawahara S ，Uenishi H ，Iwanami N ，Matsukuma H ，Abe H ，Tabata N ，Matsumura H ，Miyazawa M ... -  《INTERNATIONAL IMMUNOLOGY》

Induction of protective immunity to Friend murine leukemia virus in genetic nonresponders to virus envelope protein.

Ishihara C ，Miyazawa M ，Nishio J ，Chesebro B ... -  《-》