Effects of infection of Eimeria tenella, E. acervulina, and E. maxima upon Salmonella typhimurium infection in chickens.

Three experiments were conducted to examine whether Salmonella typhimurium infection is enhanced by concurrent infection with Eimeria tenella, E. acervulina, or E. maxima. There were two groups in each experiment: birds infected with a daily oral dose of approximately 1 X 10(4) cfu of S. typhimurium for 5 days after coccidial infection and birds infected with S. typhimurium alone with the similar exposure schedule. Chickens were necropsied 7, 10, and 14 days after coccidial infection. The numbers of S. typhimurium in the contents of ceca and small intestine were counted, and numbers of chickens positive for S. typhimurium in the liver and bile were examined. In E. tenella infection, the S. typhimurium counts in the ceca and small intestine of concurrently infected chickens were significantly greater than those of birds infected with S. typhimurium alone. In E. acervulina infection, the S. typhimurium counts in the ceca of chickens killed 14 days after E. acervulina infection and the numbers of birds positive for S. typhimurium in the ceca of chickens killed 10 and 14 days after E. acervulina infection were significantly greater than those of chickens infected with S. typhimurium alone. In E. maxima infection, the numbers of birds positive for S. typhimurium in the ceca and liver of chickens killed 7 days after E. maxima infection were significantly greater than those of birds infected with S. typhimurium alone. Results of this study indicate that infection with E. acervulina or E. maxima is able to enhance S. typhimurium infection in chickens.

Takimoto H ，Baba E ，Fukata T ，Arakawa A ... -  《-》

Establishment and persistence of Salmonella typhimurium infection stimulated by Eimeria tenella in chickens.

Baba E ，Fukata T ，Arakawa A 《RESEARCH IN VETERINARY SCIENCE》

Eimeria tenella infection enhances Salmonella typhimurium infection in chickens.

Arakawa A ，Baba E ，Fukata T 《-》

The invasion of Salmonella typhimurium into the cecal wall of chickens infected with Eimeria tenella.

The effect of concurrent Eimeria tenella and Salmonella typhimurium infections on the invasion of S. typhimurium into the cecal wall of chickens were studied. In the experiment 1) groups were composed of birds infected with a daily dose of 1 X 10(3) to 1 X 10(5) S. typhimurium for 5 days and birds infected with S. typhimurium as the same manner one day after having been infected with 20,000 E. tenella oocysts. Chickens were killed 7, 10 and 14 days after coccidial infection. The ceca were examined bacteriologically. The number of S. typhimurium in the cecal washings and cecal wall and the number of chickens positive for S. typhimurium in the ceca were significantly greater in the concurrent infections than in the S. typhimurium alone. In experiment 2) groups were composed of birds infected with E. tenella and S. typhimurium in the same manner as experiment 1) and birds infected with 2 X 10(6) to 2 X 10(7) S. typhimurium for 5 consecutive days. Chickens were killed 7, 10 and 14 (or 15) days after coccidial infection. The distribution of S. typhimurium in the ceca was examined by fluorescent antibody technique. In coccidia-infected chickens, intense, specific fluorescence was noted in the epithelia destructed by coccidia, around the coccidial oocysts parasitized in the epithelial cells, and in the lamina propria and submucosa. In S. typhimurium alone-infected chickens, specific fluorescence was also found in the epithelia, lamina propria and submucosa, but was only limited in the lumen and on the surface of epithelial linings. The results indicated that the invasion of S. typhimurium into the cecal wall was enhanced by E. tenella infection in chickens.

Hikasa Y ，Baba E ，Fukata T ，Arakawa A ... -  《-》

Growth of Salmonella typhimurium in the caecum of gnotobiotic chickens with Eimeria tenella.

Fukata T ，Baba E ，Arakawa A 《RESEARCH IN VETERINARY SCIENCE》