In-vitro and in-vivo studies of Tridax procumbens leaf extract incorporated bilayer polycaprolactone/polyvinyl alcohol-chitosan electrospun nanofiber for wound dressing application.

This study was an attempt to fabricate an antibacterial wound dressing, which was a bilayered polycaprolactone / polyvinyl alcohol-chitosan (PCL/PVA-CS) nanofibrous membrane. Entrapping ethanolic leaf extract of Tridax procumbens L. (PCL/PVA-CS/Tp). The membrane was prepared using the electrospinning technique to obtain beadless uniform nanofibers. The extract was then infused into the membrane by spraying and exposing to high temperature. in vitro antibacterial activity and cell viability of the membranes were performed. An optimized concentration of 800 μg. mL-1 of Tp extract in PCL/PVA-CS/Tp evinced better antibacterial effect on E. coli than S. aureus and also showed rapid wound closure with a positive impact on the viability of L929 cell line. The tissue regeneration efficacy of PCL/PVA-CS/Tp was validated by the experiments on mice models with subcutaneous wounds created using biopsy punch and laser radiation causing burns. Furthermore, in vivo assessments illustrated that the biopsy punch wounds healed more rapidly than laser burn though healing was significant in both. The healing processes such as anti-inflammation and re-epithelialization were observed through histological study. The upregulation of proteins namely VEGF and CD31 along with a decrease in protein levels of Wnt and TGF-β indicated significant wound healing. In conclusion, the bilayered membrane infused with plant extract could be considered a potential material for wound dressing.

Cheriyan S ，Shin H ，Razack SA ，Kang M ，Boopathi TS ，Kang HW ，Mani K ... -  《-》

Investigation of a novel bilayered PCL/PVA electrospun nanofiber incorporated Chitosan-LL37 and Chitosan-VEGF nanoparticles as an advanced antibacterial cell growth-promoting wound dressing.

Chronic wounds have become a growing concern as they can have a profound impact on individuals, potentially resulting in mortality. It is crucial to prevent and manage bacterial infections, particularly drug-resistant ones. Antimicrobial peptides, such as LL-37, can firmly eliminate pathogens. Additionally, the process of angiogenesis, facilitated by growth factors like VEGF, is essential for tissue repair and wound healing. To enhance the stability and bioavailability of therapeutic agents, targeted delivery strategies utilizing Chitosan-based carriers have been employed. Electrospun biopolymers in advanced wound dressings have revolutionized wound care by providing a more effective and efficient solution for promoting tissue regeneration and speeding up the healing process. The present investigation utilized Chitosan nanoparticles to encapsulate the recombinant LL37 peptide and VEGF. An in-depth investigation was carried out to analyze the biophysical and morphological traits of the LL37-CSNPs and VEGF-CSNPs. The first support layer consisted of PCL electrospun nanofiber, followed by the electrospinning of PVA/CsLL37, PVA/CsVEGF, and PVA/CsLL37/CsVEGF onto the PCL layer. An in vitro examination assessed the fabricated nanofibers' morphological, mechanical, and biological characteristics. The antimicrobial effects were tested on methicillin-resistant Staphylococcus aureus (MRSA). The in vivo experiments assessed the antibacterial and wound-healing capabilities of the nanofibers. The findings validated the continuous release of LL37 and VEGF. The composite material PCL/PVA/CsLL37/CsVEGF demonstrated potent bactericidal and antioxidant characteristics. The cytotoxic assay demonstrated the biocompatibility of the fabricated nano mats and their potential to accelerate fibroblast cell proliferation. The efficacy of PVA/CsLL37/CsVEGF in promoting wound healing was confirmed through an in vivo wound healing assay. Furthermore, the histological analysis provided evidence of faster epidermal formation and improved antibacterial activity in wounds covered with PVA/CsLL37/CsVEGF. Adding LL37 and VEGF to the composite material improves the immune response and promotes blood vessel formation, accelerating wound healing and decreasing inflammation.

Fahimirad S ，Khaki M ，Ghaznavi-Rad E ，Abtahi H ... -  《-》

Electrospun PCL/PVA/PHMB nanofibers incorporating Ziziphus jujuba fruit extract as promising wound dressings with potent antibacterial and antidiabetic properties.

Fahimirad S ，Satei P ，Latifi A ，Changizi-Ashtiyani S ，Bahrami M ，Abtahi H ... -  《-》

Novel electrospun chitosan/polyvinyl alcohol/zinc oxide nanofibrous mats with antibacterial and antioxidant properties for diabetic wound healing.

Ahmed R ，Tariq M ，Ali I ，Asghar R ，Noorunnisa Khanam P ，Augustine R ，Hasan A ... -  《-》

Designing New Antibacterial Wound Dressings: Development of a Dual Layer Cotton Material Coated with Poly(Vinyl Alcohol)_Chitosan Nanofibers Incorporating Agrimonia eupatoria L. Extract.

Mouro C ，Dunne CP ，Gouveia IC 《-》