Effects of refeeding with low- or high-carbohydrate diets on intermediary carbohydrate metabolism in juvenile and adult Nile tilapia.

Generally, energy expenditure and compensation according to food deprivation and refeeding often occur along the life cycle of farmed-raised fish. Fasting and refeeding are also hypothesised to modulate carbohydrate metabolism particularly for herbivorous and/or omnivorous. This study aims to investigate the effects of short-term fasting and subsequent refeeding with high or low-carbohydrate diets on the intermediary carbohydrate metabolism of juvenile and adult Nile tilapia (Oreochromis niloticus) which is known to be a good user of carbohydrate as an energy source. Fish were fasted for 4 days and subsequently refed with either a low carbohydrate and high protein (LC/HP) or high carbohydrate and low protein (HC/LP) diet for 4 days. Our results showed that 4 days of refeeding with either one of the diets could compensate for weight loss due to fasting. Thus, we investigated the effects of a 4-day-refeeding strategy and different carbohydrate-refeeding diets on plasma metabolites, nutrient composition, and glucose and its related metabolism in the liver and muscle of adult fish. Refeeding had similar effects in adults and juveniles and induced modulations to the intermediary metabolism: (1) refeeding with the HC/LP diet elevated plasma glucose levels; (2) refeeding with both diets increased triglyceride levels in the plasma, liver, and muscle, but the effect of the HC/LP diet was superior; (3) refeeding elevated plasma cholesterol levels in adults, irrespective of diet; (4) refeeding with both diets increased hepatic lipid levels in juveniles, with stronger effects observed in those fed the HC/LP diet, and refeeding with the HC/LP diet elevated hepatic lipid levels in adults; (5) refeeding with both diets increased the plasma protein content, but the effect of the LC/HP diet was superior; (6) refeeding with the LC/HP diet increased hepatic protein content in adults; and (7) refeeding with both diets increased hepatic glycogen levels, but the effect of the HC/LP diet was superior. Additionally, in juveniles and adults, refeeding with the HC/LP diet upregulated the expression of glycolytic genes in the liver and muscle, lipogenic genes in the liver, and glucose transport genes. Moreover, refeeding with the HC/LP diet downregulated the expression of gluconeogenic and amino acid catabolism genes in the liver and amino acid catabolism genes in the muscle. Collectively, the effect of short-term refeeding with a high carbohydrate diet on intermediary metabolism resembled that of long-term feeding, supporting the hypothesis that Nile tilapia, an omnivorous fish, is highly responsive to dietary carbohydrates.

Thongchaitriwat S ，Kumkhong S ，Plagnes-Juan E ，Panserat S ，Marandel L ，Boonanuntanasarn S ... -  《-》

Early feeding with hyperglucidic diet during fry stage exerts long-term positive effects on nutrient metabolism and growth performance in adult tilapia (Oreochromis niloticus).

The present study aimed to investigate nutritional programming of carbohydrate metabolism in Nile tilapia. Early nutritional intervention stimulus was achieved by feeding fry with high-protein/low-carbohydrate (HP/LC) or low-protein/high-carbohydrate (LP/HC) diet since first feeding for 4 weeks, and the effect of nutritional stimulus on carbohydrate and its related metabolism was evaluated through the adult stage. Our findings indicated that at week 1, LP/HC diet-fed fry had lower levels of mRNA for genes coding gluconeogenesis and amino acid catabolism and higher levels of hk2 (P < 0⋅05). As expected, in adult tilapia, although LP/HC diet-fed fish had poorer growth (end of stimulus), the fish showed compensatory growth. There were permanent effects of early high-carbohydrate (HC) intake on several parameters, including (1) modulating hepatic composition, (2) increased muscle glycogen, (3) lower levels of enzymes involved in amino acid catabolism and (4) higher levels of glycolytic enzymes in glycolysis. Finally, HP/LC diet- and LP/HC diet-fed fish were challenged with different dietary carbohydrate levels. Irrespective of challenging diets, the early HC stimulus had significant effects on adult tilapia by (1) promoting utilisation of glucose, which had protein-sparing effects for better growth, (2) inducting lipogenesis and (3) decreasing amino acid catabolism. Taken together, for the first time, we demonstrated that early HC feeding was effective for positive nutritional programming of metabolism in Nile tilapia (an omnivorous fish). It led to the improvement of growth performance in adult fish associated with early feeding, which is linked to a better ability to use glucose, to induce lipogenesis, and to suppress amino acid catabolism.

Kumkhong S ，Marandel L ，Plagnes-Juan E ，Veron V ，Panserat S ，Boonanuntanasarn S ... -  《-》

Alleviation of the Adverse Effect of Dietary Carbohydrate by Supplementation of Myo-Inositol to the Diet of Nile Tilapia (Oreochromis niloticus).

Zhu J ，Pan J ，Wang X ，Huang Y ，Qin C ，Qiao F ，Qin J ，Chen L ... -  《Animals》

Glucose injection into the yolk influences intermediary metabolism in adult Nile tilapia fed with high levels of carbohydrates.

Nutritional programming is a concept proposed to be applied in the field of fish nutrition to improve the use of new diets in aquaculture. This study aimed to investigate for the first time the effects of a glucose injection into the yolk at the alevin stage on intermediary metabolism and growth in adult Nile tilapia (Oreochromis niloticus) at 32-37 weeks later in the life. The early stimulus was performed through direct microinjection of 2 M glucose into yolk sacs of Nile tilapia alevin. Subsequently, in adult tilapia, the long-term effects of glucose stimulus on growth performance, blood metabolites, chemical composition in the liver and muscle, expression of genes involved in glucose transport and metabolism (glycolysis and gluconeogenesis) and related pathways (amino acid catabolism and lipogenesis) were investigated. Our results showed that, even though early glucose injection had no effect on growth performance in adult fish, very few significant effects on glucose metabolism were observed. Furthermore, to evaluate the potential metabolic programming after a dietary challenge, a 2 × 2 factorial design with two early stimuli (0.85% NaCl or 2 M glucose) and two different dietary carbohydrate intakes (medium-carbohydrate diet, CHO-M; high-carbohydrate diet, CHO-H) was performed between weeks 33 and 37. As expected, compared with the CHO-M diet, the CHO-H diet led to decreased growth performance, higher glyceamia and triglyceridemia, higher glycogen and lipid levels in the liver as well as down-regulation of gluconeogenesis and amino acid catabolism gene expressions. More interestingly, although early glucose injection had no significant effect on growth performance, it enhanced the capacities for lipogenesis, glycolysis and gluconeogenesis, particularly in fish that were fed the CHO-H diet. Thus, the nutritional programming of tilapia linked to glucose injection into the yolk of alevins is always visible at the adult stage albeit less intense than what we previously observed in juvenile.

Kumkhong S ，Marandel L ，Plagnes-Juan E ，Veron V ，Panserat S ，Boonanuntanasarn S ... -  《-》

High carbohydrate diet partially protects Nile tilapia (Oreochromis niloticus) from oxytetracycline-induced side effects.

Antibiotics used in global aquaculture production cause various side effects, which impair fish health. However, the use of dietary composition such as carbohydrate, which is one of the dominant components in fish diets to attenuate the side effects induced by antibiotics, remains unclear. We determined the ability of high carbohydrate diet to protect Nile tilapia (Oreochromis niloticus) from oxytetracycline-induced side effects. Triplicate groups of thirty O. niloticus (9.50 ± 0.08 g) were fed on medium carbohydrate (MC; 335 g/kg) and high carbohydrate (HC; 455 g/kg) diets without and with 2.00 g/kg diet of oxytetracycline (80 mg/kg body weight/day) hereafter, MCO and HCO for 35 days. Thereafter, we assessed growth performance, hepatic nutrients composition and metabolism, microbiota abundance, immunity, oxidative and cellular stress, hepatotoxicity, lipid peroxidation and apoptosis. To understand the possible mechanism of carbohydrate protection on oxytetracycline, we assessed the binding effects and efficiencies of mixtures of medium and high starch with oxytetracycline as well as the MCO and HCO diets. The O. niloticus fed on the MCO and HCO diets had lower growth rate, nutrients utilization and survival rate than those fed on the MC and HC diets, respectively. Dietary HCO increased hepatosomatic index and hepatic protein content of O. niloticus than MCO diet. The O. niloticus fed on the HCO diet had lower mRNA expression of genes related to protein, glycogen and lipid metabolism compared to those fed on the MCO diet. Feeding O. niloticus on the HCO diet increased innate immunity and reduced pathogenic bacteria, pro-inflammation, hepatotoxicity, cellular stress and apoptosis than the MCO diet. The high starch with oxytetracycline and HCO diet had higher-oxytetracycline binding effects and efficiencies than the medium starch with oxytetracyline and MCO diet, respectively. Our study demonstrates that, high carbohydrate partially protects O. niloticus from oxytetracycline-induced side effects by binding the antibiotic. Incorporating high carbohydrate in diet formulation for omnivorous fish species alleviates some of the side effects caused by antibiotics.

Limbu SM ，Zhang H ，Luo Y ，Chen LQ ，Zhang M ，Du ZY ... -  《-》