pSPObooster: A Plasmid System to Improve Sporulation Efficiency of Saccharomyces cerevisiae Lab Strains.

Common Saccharomyces cerevisiae lab yeast strains derived from S288C have meiotic defects and therefore are poor sporulators. Here, we developed a plasmid system containing corrected alleles of the MKT1 and RME1 genes to rescue the meiotic defects and show that standard BY4741 and BY4742 strains containing the plasmid display faster and more efficient sporulation. The plasmid, pSPObooster, can be maintained as an episome and easily cured or stably integrated into the genome at a single locus. We demonstrate the use of pSPObooster in low- and high-throughput yeast genetic manipulations and show that it can expedite both procedures without impacting strain behavior.

Loll-Krippleber R ，Jiang YK ，Brown GW 《-》

Temporal expression profiling identifies pathways mediating effect of causal variant on phenotype.

Gupta S ，Radhakrishnan A ，Raharja-Liu P ，Lin G ，Steinmetz LM ，Gagneur J ，Sinha H ... -  《PLoS Genetics》

Natural isolates of Saccharomyces cerevisiae display complex genetic variation in sporulation efficiency.

Gerke JP ，Chen CT ，Cohen BA 《GENETICS》

IME4, a gene that mediates MAT and nutritional control of meiosis in Saccharomyces cerevisiae.

Shah JC ，Clancy MJ 《-》

Dependence of inessential late gene expression on early meiotic events in Saccharomyces cerevisiae.

Kao G ，Mannix DG ，Holaway BL ，Finn MC ，Bonny AE ，Clancy MJ ... -  《molecular and general genetics》