Hyaluronan and proteoglycan link protein 1 - a novel signaling molecule for rejuvenating aged skin.

The skin seems to rejuvenate upon exposure to factors within the circulation of young organisms. Intrinsic factors that modulate skin aging are poorly understood. We used heterochronic parabiosis and aptamer-based proteomics to identify serum-derived rejuvenating factors. We discovered a novel extracellular function of hyaluronan and proteoglycan link protein 1 (HAPLN1). Its serum levels decreased with age, disturbing the integrity of the skin extracellular matrix, which is predominantly composed of collagen I and hyaluronan; levels of various markers, which decrease in aged skin, were significantly restored in vivo and in vitro by the administration of recombinant human HAPLN1 (rhHAPLN1). rhHAPLN1 protected transforming growth factor beta receptor 2 on the cell surface from endocytic degradation via mechanisms such as regulation of viscoelasticity, CD44 clustering, and hyaluronan cross-linking. Moreover, rhHAPLN1 regulated the levels of nuclear factor erythroid 2-related factor 2, phosphorylated nuclear factor kappa B, and some cyclin-dependent kinase inhibitors such as p16 and p21. Therefore, rhHAPLN1 may act as a novel biomechanical signaling protein to rejuvenate aged skin.

Fu Z ，Yang G ，Yun SY ，Jang JM ，Ha HC ，Shin IC ，Back MJ ，Piao Y ，Kim DK ... -  《-》

Recombinant Human HAPLN1 Mitigates Pulmonary Emphysema by Increasing TGF-β Receptor I and Sirtuins Levels in Human Alveolar Epithelial Cells.

Piao Y ，Yun SY ，Fu Z ，Jang JM ，Back MJ ，Kim HH ，Kim DK ... -  《-》

Novel Effect of Hyaluronan and Proteoglycan Link Protein 1 (HAPLN1) on Hair Follicle Cells Proliferation and Hair Growth.

Ha HC ，Zhou D ，Fu Z ，Back MJ ，Jang JM ，Shin IC ，Kim DK ... -  《-》

Comparison of sialylated and fucosylated N-glycans attached to Asn 6 and Asn 41 with different roles in hyaluronan and proteoglycan link protein 1 (HAPLN1).

Park CS ，Moon C ，Kim M ，Kim J ，Yang S ，Jang L ，Jang JY ，Jeong CM ，Lee HS ，Kim DK ，Kim HH ... -  《-》

Versican/PG-M Assembles Hyaluronan into Extracellular Matrix and Inhibits CD44-mediated Signaling toward Premature Senescence in Embryonic Fibroblasts.

Suwan K ，Choocheep K ，Hatano S ，Kongtawelert P ，Kimata K ，Watanabe H ... -  《-》