Do nanoplastics impact Pb up-taking by Hordeum vulgare L.?

Most studies on nanoplastics (NPs) focus on aquatic environments, overlooking their combined bioaccumulation with pollutants in terrestrial ecosystems. This study addresses a part of this gap by investigating how polystyrene nanoplastics (PS-NPs) affect the bioaccumulation and translocation of lead (Pb) in Hordeum vulgare L. plants. Using the RHIZOtest device for precise soil contamination control, we quantified PS-NPs (50 nm) in plant shoots via pyrolysis-gas chromatography/mass spectrometry (Py-GCMS) after plant KOH digestion. Our findings revealed that PS-NPs reduce Pb bioaccumulation and make adsorbed Pb onto PS-NPs less bioavailable to plants. For the highest Pb concentration, the Pb uptake index (PUI) followed the trend: Free Pb > NPs + Pb > Pb primary adsorbed by NPs, showing reduced Pb translocation to shoots in the presence of PS-NPs. Moreover, the presence of Pb decreased the bioavailability of PS-NPs probably in response to PS-NPs aggregation or modified charge. The PS-NPs concentrations in shoots range from 275.2 to 400 μg g-1, representing 3.9 to 5.75% of the total PS-NPs. This study highlights the intricate interactions between nanoplastics and metals in soil-plant systems and emphasizes the need for further research on their combined effects and potential risks to food safety.

Ryzhenko N ，Dutruch L ，Tabo B ，Pecheul G ，Pattier M ，Khatib I ，Pédrot M ，Gigault J ，Cabello-Hurtado F ，El Amrani A ，Davranche M ... -  《NanoImpact》

Low temperature tolerance is impaired by polystyrene nanoplastics accumulated in cells of barley (Hordeum vulgare L.) plants.

With increasing plastic consumption, the large amount of polystyrene nanoplastics (PS-NPs) in agricultural soil may not only directly affect the plant growth, but also indirectly affect the abiotic stress tolerance in crops. In this study, the barley (Hordeum vulgare L.) was irrigated with 2 g L-1 PS-NPs (65.776 ± 0.528 nm) solution for 7 days, then subjected to low temperature (2 ℃) for 24 h. The imaging of protoplasts indicated that polymethylmethacrylate nanoplastics could across the cell wall and accumulate in plant cells. The PS-NPs significantly decreased Rubisco activities and ATP production, hence limiting the photosynthetic carbon assimilation in barley under low temperature. The PS-NPs accumulated in cells also caused the significantly decreased activities of key enzymes involved in sucrolytic, glycolysis and starch metabolism pathways, including UDP-glucose pyrophorylase, ADP-Glucose pyrophosphorylase, phosphoglucomutase, glucose-6-phosphate dehydrogenase, phosphoglucoisomerase, fructokinase and phosphofructokinase. In addition, under low temperature, the PS-NPs presence significantly reduced the activities of superoxide dismutase, ascorbate peroxidase and catalase in chloroplasts, and significantly reduced the activities of ascorbate peroxidase and catalase in mitochondria. Thus, it is suggested that the PS-NPs accumulated in plant cells impaired the low temperature tolerance in barley mainly by the negative effects on photosynthetic carbon assimilation, carbohydrate metabolism and ROS homeostasis in sub-cellular level.

Wang Z ，Li S ，Jian S ，Ye F ，Wang T ，Gong L ，Li X ... -  《-》

Heterogeneity effects of nanoplastics and lead on zebrafish intestinal cells identified by single-cell sequencing.

Plastic particles in water environment can adsorb heavy metals, leading to combined toxicity on aquatic organisms. However, current conclusions are mostly obtained based on cell population-average responses. Heterogeneity effects among cell populations in aquatic organisms remain unclear. This study firstly analyzed the basic toxic effects of 20 μg L-1, 200 μg L-1 100 nm polystyrene nanoplastics (PS-NPs), 50 μg L-1 lead (Pb), and their combined exposures on zebrafish intestine. Results found that combined exposure of 200 μg L-1 PS-NPs and 50 μg L-1 Pb induced highest MDA, 8-OHdG, and TNF-α levels. Thus 200 μg L-1 PS-NPs, 50 μg L-1 Pb and their combined exposures were chosen to analyze the heterogeneity effects on zebrafish intestine cells by single-cell RNA sequencing. A total of 38,640 zebrafish intestinal cells were obtained and identified as seven cell populations, including enterocytes, macrophages, neutrophils, B cells, T cells, enteroendocrine cells, and goblet cells. 200 μg L-1 PS-NPs exposure had the greatest influence on macrophages, while Pb exposure mostly influenced enterocytes. Results of MDA, 8-OHdG, and TNF-α analyses indicated that 20 μg L-1 and 200 μg L-1 PS-NPs increased the Pb toxicity. However, the scRNA-seq showed that the synergistic effects did not exist in most cell populations, except for goblet cells. Co-exposure of 200 μg L-1 PS-NPs and Pb caused similar transcriptome profiles with 200 μg L-1 PS-NPs exposure in macrophages, which changed immunological recognition and apoptosis processes. The Pb exposure influenced the macrophages by direct cytotoxicity. However, the Pb alone and combined exposures induced similar toxicities in the enterocytes, including the generation of oxidative stress and abnormality of lipid metabolism. This study shows the scRNA-seq is a powerful method to identify the target cell populations and corresponding toxic effects during combined exposure of pollutants.

Yu J ，Chen L ，Gu W ，Liu S ，Wu B ... -  《-》

Palladium-doped and undoped polystyrene nanoplastics in a chronic toxicity test for higher plants: Impact on soil, plants and ammonium oxidizing bacteria.

Hoppe M ，Köser J ，Scheeder G ，Lamparter A ，Dorau K ，Grüger L ，Dierkes G ，Schlich K ... -  《NanoImpact》

Different effects and mechanisms of polystyrene micro- and nano-plastics on the uptake of heavy metals (Cu, Zn, Pb and Cd) by lettuce (Lactuca sativa L.).

Heavy metals are widely distributed in soil ecosystems, posing a potential threat to soil biota. Micro- and nano-plastics (MNPs) can impact the accumulation of heavy metals in plants through changing soil microbial community and cause injury to plants. In this work, two concentrations (100 and 1000 mg/kg) polystyrene microplastics (PS-MPs) and nanoplastics (PS-NPs) were adopted to explore the effects and mechanisms of MNPs on the uptake of Cu, Zn, Pb and Cd in lettuce (Lactuca sativa L.). MPs increased the uptake of heavy metals in lettuce by increasing the relative abundance of the key metal-activation bacteria in rhizospheric soil. At the end of experiment, the contents of Cu, Zn, Pb and Cd in NP treatments were significantly (p < 0.05) higher than that of MPs, particularly in 1000 mg/kg of NPs, with concentrations of 52.6, 174, 10.3, and 33.2 mg/kg, respectively. Biomarkers and gene expression reveled that 1000 mg/kg of NPs caused more severe injuries to lettuce plant at the end. Moreover, metabolomic analysis demonstrated that NPs disturbed the metabolism of ATP-binding cassette transporter (ABC transporter) and plant hormone signal transduction of lettuce root, causing increased uptake of heavy metals by lettuce. This work reveals that MPs may increase accumulation of heavy metals by altering the rhizosphere microorganisms, whereas NPs increase accumulation of heavy metals by causing more severe injuries to lettuce plant.

Xu G ，Lin X ，Yu Y 《-》