Dog leukocyte antigen genotyping across class I and class II genes in beagle dogs as laboratory animals.

Dog leukocyte antigen (DLA) polymorphisms have been found to be associated with inter-individual variations in the risk, susceptibility, and severity of immune-related phenomena. While DLA class II genes have been extensively studied, less research has been performed on the polymorphisms of DLA class I genes, especially in beagle dogs commonly used as laboratory animals for safety evaluations in drug development. We genotyped four DLA class I genes and four DLA class II genes by locus-specific Sanger sequencing using 93 laboratory beagle dogs derived from two different strains: TOYO and Marshall. The results showed that, for DLA class I genes, 11, 4, 1, and 2 alleles, including a novel allele, were detected in DLA-88, DLA-12/88L, DLA-64, and DLA-79, while, for DLA class II genes, 1, 10, 6, and 7 alleles were detected in DLA-DRA, DLA-DRB1, DLA-DQA1, and DLA-DQB1, respectively. It was estimated that there were 14 DLA haplotypes, six of which had a frequency of ≥ 5%. Furthermore, when comparing the DLA diversity between TOYO and Marshall strains, the most common alleles and haplotypes differed between them. This is the first study to genotype all DLA loci and determine DLA haplotypes including all DLA class I and class II genes in dogs. Integrating information on the DLA diversity of laboratory beagle dogs should reinforce their benefit as an animal model for understanding various diseases associated with a specific DLA type.

Konno H ，Miyamae J ，Kataoka H ，Akai M ，Miida H ，Tsuchiya Y ... -  《-》

Large-Scale Polymorphism Analysis of Dog Leukocyte Antigen Class I and Class II Genes (DLA-88, DLA-12/88L and DLA-DRB1) and Comparison of the Haplotype Diversity between Breeds in Japan.

Miyamae J ，Okano M ，Katakura F ，Kulski JK ，Moritomo T ，Shiina T ... -  《Cells》

Identification of novel polymorphisms and two distinct haplotype structures in dog leukocyte antigen class I genes: DLA-88, DLA-12 and DLA-64.

Miyamae J ，Suzuki S ，Katakura F ，Uno S ，Tanaka M ，Okano M ，Matsumoto T ，Kulski JK ，Moritomo T ，Shiina T ... -  《-》

Extensive interbreed, but minimal intrabreed, variation of DLA class II alleles and haplotypes in dogs.

Kennedy LJ ，Barnes A ，Happ GM ，Quinnell RJ ，Bennett D ，Angles JM ，Day MJ ，Carmichael N ，Innes JF ，Isherwood D ，Carter SD ，Thomson W ，Ollier WE ... -  《-》

Frequency and distribution of alleles of canine MHC-II DLA-DQB1, DLA-DQA1 and DLA-DRB1 in 25 representative American Kennel Club breeds.

Angles JM ，Kennedy LJ ，Pedersen NC 《-》