Fullerenols Ameliorate Social Deficiency and Rescue Cognitive Dysfunction of BTBR T(+)Itpr3(tf)/J Autistic-Like Mice.

Autism Spectrum Disorder (ASD) is a neurodevelopmental condition that affects social interaction and communication and can cause stereotypic behavior. Fullerenols, a type of carbon nanomaterial known for its neuroprotective properties, have not yet been studied for their potential in treating ASD. We aimed to investigate its role in improving autistic behaviors in BTBR T+Itpr3tf/J (BTBR) mice and its underlying mechanism, which could provide reliable clues for future ASD treatments. Our research involved treating C57BL/6J (C57) and BTBR mice with either 0.9% NaCl or fullerenols (10 mg/kg) daily for one week at seven weeks of age. We then conducted ASD-related behavioral tests in the eighth week and used RNA-seq to screen for vital pathways in the mouse hippocampus. Additionally, we used real-time quantitative PCR (RT-qPCR) to verify related pathway genes and evaluated the number of stem cells in the hippocampal dentate gyrus (DG) by Immunofluorescence staining. Our findings revealed that fullerenols treatment significantly improved the related ASD-like behaviors of BTBR mice, manifested by enhanced social ability and improved cognitive deficits. Immunofluorescence results showed that fullerenols treatment increased the number of DCX+ and SOX2+/GFAP+ cells in the DG region of BTBR mice, indicating an expanded neural progenitor cell (NPC) pool of BTBR mice. RNA-seq analysis of the mouse hippocampus showed that VEGFA was involved in the rescued hippocampal neurogenesis by fullerenols treatment. In conclusion, our findings suggest that fullerenols treatment improves ASD-like behavior in BTBR mice by upregulating VEGFA, making nanoparticle- fullerenols a promising drug for ASD treatment.

Luo J ，Luo Y ，Zhao M ，Liu Y ，Liu J ，Du Z ，Gong H ，Wang L ，Zhao J ，Wang X ，Gu Z ，Zhao W ，Liu T ，Fan X ... -  《International Journal of Nanomedicine》

Neonatal curcumin treatment restores hippocampal neurogenesis and improves autism-related behaviors in a mouse model of autism.

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Taurine Improved Autism-Like Behaviours and Defective Neurogenesis of the Hippocampus in BTBR Mice through the PTEN/mTOR/AKT Signalling Pathway.

Xiaoyan H ，Zhaoxi Y ，Lingli Z ，Jinyuan C ，Wen Q ... -  《FOLIA BIOLOGICA》

Cadmium exposure exacerbates immunological abnormalities in a BTBR T(+) Itpr3(tf)/J autistic mouse model by upregulating inflammatory mediators in CD45R-expressing cells.

Albekairi TH ，Alanazi MM ，Ansari MA ，Nadeem A ，Attia SM ，Bakheet SA ，Al-Mazroua HA ，Aldossari AA ，Almanaa TN ，Alwetaid MY ，Alqinyah M ，Alnefaie HO ，Ahmad SF ... -  《-》

Human amniotic epithelial cell transplantation promotes neurogenesis and ameliorates social deficits in BTBR mice.

Zhang R ，Cai Y ，Xiao R ，Zhong H ，Li X ，Guo L ，Xu H ，Fan X ... -  《-》