Differential lncRNA profiles of blood plasma-derived exosomes from systemic lupus erythematosus.

Long non-coding RNAs (lncRNAs) dysregulation is key in the pathogenesis of systemic lupus erythematosus (SLE), but the role of exosomal lncRNAs in SLE has not been well studied. We elucidated the profiles of plasma exosomal lncRNAs expression in patients with SLE and predictd their potential clinical significance in SLE. In the screening stage, six newly diagnosed and untreated patients with SLE and six healthy controls were examined by high-throughput sequencing technology, and differential exosomal lncRNA profiles were constructed. In the validation phase, two differentially selected exosomal lncRNAs from 20 patients each with active and stable SLE and 20 healthy controls were verified with RT-qPCR. The correlation between the selected exosomal lncRNAs and SLE clinical indicators was examined. The diagnostic value of the selected exosomal lncRNAs in SLE was analyzed by the receiver operator characteristic (ROC) curve. Exosomes were successfully extracted from the patients and controls. Sequencing-phase sequencing demonstrated 528 upregulated lncRNAs and 7491 downregulated lncRNAs. In the validation stage, exosomal LINC00667 and DANCR were significantly upregulated in the patients, and positively correlated with Systemic Lupus Erythematosus Disease Activity Index 2000 (SLEDAI-2 K). Exosomal DANCR expression between the active and stable SLE patients was different. The area under the curve(AUC) of exosomal LINC00667 and DANCR for SLE diagnosis was 0.815 and 0.759, respectively. Exosomal LINC00667 and DANCR were upregulated in SLE, and might be new biomarkers thereof. Exosomal DANCR was associated with SLE activity.

Peng XC ，Ma LL ，Miao JY ，Xu SQ ，Shuai ZW ... -  《-》

A novel long noncoding RNA ENST00000597482 serves as a potential biomarker for disease activity and diagnosis of systemic lupus erythematosus.

Wang C ，Yuan S ，Zeng Y ，Li W ，Ye J ，Li F ，He Z ，Chen Y ，Lin X ，Liang L ，Xu H ，Cai X ... -  《-》

Differential Plasma Expression Profiles of Long Non-Coding RNAs Reveal Potential Biomarkers for Systemic Lupus Erythematosus.

Wu GC ，Hu Y ，Guan SY ，Ye DQ ，Pan HF ... -  《Biomolecules》

Full high-throughput sequencing analysis of differences in expression profiles of long noncoding RNAs and their mechanisms of action in systemic lupus erythematosus.

Ye H ，Wang X ，Wang L ，Chu X ，Hu X ，Sun L ，Jiang M ，Wang H ，Wang Z ，Zhao H ，Yang X ，Wang J ... -  《-》

Differential expressions and potential clinical values of lncRNAs in the plasma exosomes of rheumatoid arthritis.

Shuai ZQ ，Wang ZX ，Ren JL ，Yang XK ，Xu B ... -  《-》