Magnetic field-assisted surface engineering technology for active regulation of Fe(3)O(4) medium to enable the laccase electrochemical biosensing of catechol with visible stripe patterns.

Catechol (CC), a prevalent phenolic compound, is a byproduct in various agricultural, chemical, and industrial processes. CC detection is crucial for safeguarding water quality and plays a pivotal role in enhancing the overall quality of life of individuals. Electrochemical biosensors exhibit rapid responses, have small sizes, and can be used for real-time monitoring. Therefore, the development of a fast and sensitive electrochemical biosensor for CC detection is crucial. In this study, a laccase-based electrochemical biosensor for detection of CC is successfully developed using Fe3O4 nanoparticles as medium and optimized by applying a magnetic field. This research proposes a unique strategy for biosensor enhancement by actively controlling the distribution of magnetic materials on the electrode surface through the application of a magnetic field, resulting in a visibly alternating stripe pattern. This approach effectively disperses magnetic particles, preventing their aggregation and reducing the boundary layer thickness, enhancing the electrochemical response of the biosensor. After fabrication condition optimization, CC is successfully detected using this biosensor. The fabricated sensor exhibits excellent performance with a wide linear detection range of 10-1000 μM, a low detection limit of 1.25 μM, and a sensitivity of 7.9 μA/mM. The fabricated sensor exhibits good selectivity and reliable detection in real water samples. In addition, the laccase-based sensor has the potential for the fast and accurate monitoring of CC in olive oil. The magnetic field optimization in this study significantly improved the performance of the electrochemical biosensor for detecting CC in environmental samples. Overall, the sensor developed in this study has the potential for fast and accurate monitoring of CC in environmental samples, highlighting the potential importance of a magnetic field environment in improving the performance of catechol electrochemical biosensors.

Wang F ，Zhang J ，Xu L ，Ma A ，Zhuang G ，Huo S ，Zou B ，Qian J ，Cui Y ，Zhang W ... -  《-》

A novel amperometric catechol biosensor based on α-Fe(2)O(3) nanocrystals-modified carbon paste electrode.

Sarika C ，Shivakumar MS ，Shivakumara C ，Krishnamurthy G ，Narasimha Murthy B ，Lekshmi IC ... -  《-》

Laccase immobilization on the electrode surface to design a biosensor for the detection of phenolic compound such as catechol.

Biosensors based on the coupling of a biological entity with a suitable transducer offer an effective route to detect phenolic compounds. Phenol and phenolic compounds are among the most toxic environmental pollutants. Laccases are multi-copper oxidases that can oxide phenol and phenolic compounds. A method is described for construction of an electrochemical biosensor to detect phenolic compounds based on covalent immobilization of laccase (Lac) onto polyaniline (PANI) electrodeposited onto a glassy carbon (GC) electrode via glutaraldehyde coupling. The modified electrode was characterized by voltammetry, Fourier transform infrared (FTIR) spectroscopy and atomic force microscopy (AFM) techniques. The results indicated that laccase was immobilized onto modified GC electrode by the covalent interaction between laccase and terminal functional groups of the glutaraldehyde. The laccase immobilized modified electrode showed a direct electron transfer reaction between laccase and the electrode. Linear range, sensitivity, and detection limit for this biosensor were 3.2 × 10(-6) to 19.6 × 10(-6)M, 706.7 mAL mol(-1), 2.07 × 10(-6)M, respectively.

Nazari M ，Kashanian S ，Rafipour R 《-》

[Catechol biosensor based on immobilizing laccase to modified core-shell magnetic nanoparticles supported on carbon paste electrode].

Zhang Y ，Zeng GM ，Tang L ，Yu HY ，Li JB ... -  《-》

Biosensors Platform Based on Chitosan/AuNPs/Phthalocyanine Composite Films for the Electrochemical Detection of Catechol. The Role of the Surface Structure.

Salvo-Comino C ，González-Gil A ，Rodriguez-Valentin J ，Garcia-Hernandez C ，Martin-Pedrosa F ，Garcia-Cabezon C ，Rodriguez-Mendez ML ... -  《-》