Effects of polyurethane microplastics combined with cadmium on maize growth and cadmium accumulation under different long-term fertilisation histories.

Agricultural production uses different types of fertilisation treatments, typically employing the combined application of organic fertiliser (OF) or organic-inorganic fertiliser (OIF) to improve soil quality. When coupled with cadmium (Cd), microplastics (MPs) affect plant growth and Cd accumulation in soils treated with different fertilisers. This study systematically examined the effects of polyurethane (PU) MPs coupled with Cd on the growth characteristics, root metabolite characteristics, rhizosphere bacterial community structure, and Cd bioavailability of maize under different long-term fertilisation treatments and soil types (red/cinnamon soil). The combined effects of PU MPs and Cd on maize growth differed across fertilisation treatments. Under OF, maize plants accumulated more Cd than under OIF. The accumulation of Cd in maize plants in red soil was twice that in cinnamon soil. Under OF, PU MPs promoted Cd activation by decreasing the soil pH, while root metabolites promoted Cd adsorption sites by synthesising specific amino acids, degrading aromatic compounds, and synthesising pantothenic acid and coenzyme A. Under OF, PU MPs can lower the soil pH to promote the activation of cadmium, while root metabolites promote root growth and increase cadmium adsorption sites by synthesizing specific amino acids, degrading aromatic compounds, and synthesizing pantothenic acid and coenzyme A, hereby promoting root Cd absorption. Under OIF, PU MPs act by influencing the biosynthesis of amino acids in root metabolites, enriching energy metabolism pathways, promoting the transport and translocation of mineral nutrients, thereby amplifying the "toxic effects" of Cd. This study provides new insights into the risk assessment of PU MPs and Cd coupling under different fertilisation treatments, and suggests that the prevention and control of combined PU MPs and Cd pollution in red soil under OF treatment should receive more attention in the future.

Zhao M ，Li Y ，Li C ，Wang X ，Cao B ，Zhang J ，Wang J ，Zou G ，Chen Y ... -  《-》

Effect of biodegradable microplastics and Cd co-pollution on Cd bioavailability and plastisphere in soil-plant system.

Biodegradable plastics (BPs) are regarded as ecomaterials and are emerging as a substitute for traditional non-degradable plastics. However, the information on the interaction between biodegradable microplastics (BMPs) and cadmium (Cd) in agricultural soil is still limited. Here, lettuce plants were cultured in BMPs (polylactic acid (PLA) MPs and poly(butylene-adipate-co-terephthalate) (PBAT) MPs) and Cd co-polluted soil for 35 days. The results show that diffusive gradient in thin films technique (DGT) but not diethylenetriaminepentaacetic acid (DTPA) extraction method greatly improved the prediction reliability of Cd bioavailability in non-rhizosphere soil treated with BMPs (R2 = 0.902). BMPs increased the Cd bioavailability in non-rhizosphere soil indirectly by decreasing soil pH, cation exchange capacity (CEC), and dissolved organic carbon (DOC), rather than by directly adsorbing Cd on their surface. PLA MPs incubated in rhizosphere soil showed more considerable degradation with extremely obvious cavities and the fracture of ester functional groups on their surface than PBAT MPs. BMPs could provide ecological niches to colonize and induce microorganisms associated with BMPs' degradation to occupy a more dominant position. In addition, Cd only affected the composition and function of microbial communities in soil but not on BMPs. However, co-exposure to BMPs and Cd significantly reduced the degrees of co-occurrence network of fungal communities on PLA MPs and PBAT MPs by 37.7% and 26.7%, respectively, compared to single exposure to BMPs.

Shao X ，Liang W ，Gong K ，Qiao Z ，Zhang W ，Shen G ，Peng C ... -  《-》

Chemotaxis of rhizosphere Pseudomonas sp. induced by foliar spraying of lanthanum reduces cadmium uptake by pakchoi.

Foliar application of rare earth micronutrient of lanthanum (La) exhibits great potential in reducing cadmium (Cd) uptake in crops, the underlying mechanisms controlling the interaction between Cd toxicity-relieved crops and soil microbiota are poorly understood. In this study, LaCl3 with the concentrations of 10 and 30 μM was sprayed on pakchoi (Brassica chinensis L.) planting on Cd contaminated solution and soil to determine the changes of root metabolites and rhizosphere bacterial communities. Compared to the control, Cd concentration in pakchoi leaves was significantly decreased by 30.9 % and 22.6 % with the high group under both hydroponic and pot culture by applying 30 μM LaCl3. Herein, the concrete evidence is provided that pakchoi plants in response to foliar-spraying La under soil or solution Cd toxicity can promote the root secretion of amino acids, resulting in a strong enrichment of nitrogen-related microorganisms. To probe this linkage, a Pseudomonas representative specie was isolated that had the ability of consuming alanine, the most oversecreted root exudate due to La application. Further results demonstrated that this strain had the capacities for alleviating Cd toxicity and enhancing crop growth by immobilizing Cd and secreting plant-beneficial metabolites. This study reveals a plant-extrudate-microbiome feedback loop for responding to La-relieved Cd toxicity in crops by the chemotaxis of rhizosphere Pseudomonas toward alanine secreted by pakchoi.

Wang M ，Yu L ，Wang J ，Qin L ，Sun X ，Liu J ，Han Y ，Chen S ... -  《-》

Combined contamination of microplastic and antibiotic alters the composition of microbial community and metabolism in wheat and maize rhizosphere soil.

The widespread application of antibiotics and plastic films in agriculture has led to new characteristics of soil pollution. The impacts of combined contamination of microplastics and antibiotics on plant growth and rhizosphere soil bacterial community and metabolisms are still unclear. We conducted a pot experiment to investigate the effects of polyethylene (0.2%) and norfloxacin/doxycycline (5 mg kg-1), as well as the combination of polyethylene and antibiotics, on the growth, rhizosphere soil bacterial community and metabolisms of wheat and maize seedlings. The results showed that combined contamination caused more serious damage to plant growth than individual contamination, and aggravated root oxidative stress responses. The diversity and structure of soil bacterial community were not markedly altered, but the composition of the bacterial community, soil metabolisms and metabolic pathways were altered. The co-occurrence network analysis indicated that combined contamination may inhibit the growth of wheat and maize seedings by simplifying the interrelationships between soil bacteria and metabolites, and altering the relative abundance of specific bacteria genera (e.g. Kosakonia and Sphingomonas) and soil metabolites (including sugars, organic acids and amino acids). The results help to elucidate the potential mechanisms of phytotoxicity of the combination of microplastic and antibiotics.

Zhang Z ，Zhao L ，Jin Q ，Luo Q ，He H ... -  《-》

[Interactional Effects of Soil and Foliar Application of Manganese Combined with Soil Stabilizer on Cd Accumulation in Rice].

Qiu YH ，Tang YL ，Li ZG ，Chen CM ，Wei ZB ，Guo YJ ，Wu QT ... -  《-》