RHBDD1 Promotes the Growth and Stemness Characteristics of Gastric Cancer Cells by Activating Wnt/β-catenin Signaling Pathway.

Gastric cancer (GC) is threatening public health, with at least one million new cases reported each year. Rhomboid domain-containing protein 1 (RHBDD1) has been identified to regulate the proliferation, migration, and metastasis of cancer cells. However, the role of RHBDD1 in GC has not been elucidated. This study aimed to investigate the role of RHBDD1 on the growth, metastasis, and stemness characteristics of GC. RHBDD1 expression was analyzed from the TCGA databank. qRT-PCR was conducted to evaluate the transcription level of RHBDD1. Western blots were used to evaluate the protein expression of RHBDD1, CD133, CD44, Nanog, β-catenin and c-myc. Colony formation assay and transwell assay were conducted to evaluate the growth and metastasis of NCI-N87 cells, respectively. Sphere-forming assay was performed to study the stemness characteristics. The nude mice xenotransplantation model and immunohistochemistry (IHC) were performed to evaluate the growth of GC in vivo. Results: RHBDD1 expression is elevated in GC cells and clinical tissues. RHBDD1 expression is positively associated with cell proliferation and metastasis of GC cells. RHBDD1 knockdown suppresses the expression of CD133, CD44 and Nanog and attenuates sphere-forming ability. RHBDD1 activates the Wnt/β-catenin pathway via promoting the expression of β-catenin / c-myc and inducing β-catenin translocation into nuclear. RHBDD1 knockdown inhibits the growth of GC in nude mice xenotransplantation model. RHBDD1 is highly expressed in GC, and its knockdown inhibits the growth, metastasis and stemness characteristics of GC cells through activating the Wnt/β-catenin pathway, suggesting that RHBDD1 has the potential to be a novel therapeutic target for GC treatment.

Yang Y ，Yuan Y ，Xia B 《-》

RHBDD1 promotes colorectal cancer metastasis through the Wnt signaling pathway and its downstream target ZEB1.

Zhang M ，Miao F ，Huang R ，Liu W ，Zhao Y ，Jiao T ，Lu Y ，Wu F ，Wang X ，Wang H ，Zhao H ，Ju H ，Miao S ，Wang L ，Song W ... -  《JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH》

TRIM28 suppresses cancer stem-like characteristics in gastric cancer cells through Wnt/β-catenin signaling pathways.

Ning T ，Zhao M ，Zhang N ，Wang Z ，Zhang S ，Liu M ，Zhu S ... -  《-》

MARCH1 promotes the growth and maintaining of stem cell-like characteristics of gastric cancer cells by activating the Wnt/β-catenin signaling pathway.

Gastric carcinoma (GC) is a malignant tumor, which is an important cause of death in all tumor deaths. The role of MARCH1 in GC has not been studied, this study aims to investigate the function of MARCH1 in GC. The expression of MARCH1 in normal tissue and tumor tissue was analyzed by TCGA-based GEPIA platform and UALCAL website and verified by RT-qPCR, Western blotting (WB), and Immunohistochemistry (IHC); CCK8 assay and crystal violet assay were separately used to detect cell viability and cell cloning ability. Cell spheroidization assay and Fluorescence-activated cell sorting (FACS) were performed to determine CD44+, CD133+ cell numbers to study the stemness characteristics of GC cells. While, WB was used to study the specific signaling pathway regulated by MARCH1. Animal model of GC was established to study the regulation of MARCH1 on GC growth in vivo. It showed that the expression of MARCH1 in GC tissues was higher than that in normal tissues; CCK8 and crystal violet assay showed that MARCH1 could promote cell viability and cloning ability of GC cells; cell spheroidization experiments and FACS showed that MARCH1 promoted the cloning ability of GC cells; WB results revealed that MARCH1 mainly regulated GC through the Wnt/β-catenin signaling pathway; In-vivo results showed that MARCH1 can promote the growth of GC. This study found that MARCH1 maintained the stemness characteristics and promoted the proliferation of GC cells by activating the Wnt/β-catenin signaling pathway.

Liang W ，Zhang T ，Huo J ，Yang J ... -  《-》

SUFU mediates EMT and Wnt/β-catenin signaling pathway activation promoted by miRNA-324-5p in human gastric cancer.

Peng Y ，Zhang X ，Lin H ，Deng S ，Qin Y ，Yuan Y ，Feng X ，Wang J ，Chen W ，Hu F ，Yan R ，Zhao Y ，Cheng Y ，Wei Y ，Fan X ，Ashktorab H ，Smoot D ，Li S ，Meltzer SJ ，Jin Z ... -  《-》