Biological treatment of triclosan using a novel strain of Enterobacter cloacae and introducing naphthalene dioxygenase as an effective enzyme.

In recent years, triclosan (TCS) has been widely used as an antibacterial agent in personal care products due to the spread of the Coronavirus. TSC is an emerging contaminant, and due to its stability and toxicity, it cannot be completely degraded through traditional wastewater treatment methods. In this study, a novel strain of Enterobacter cloacae was isolated and identified that can grow in high TCS concentrations. Also, we introduced naphthalene dioxygenase as an effective enzyme in TCS biodegradation, and its role during the removal process was investigated along with the laccase enzyme. The change of cell surface hydrophobicity during TCS removal revealed that a glycolipid biosurfactant called rhamnolipid was involved in TCS removal, leading to enhanced biodegradation of TCS. The independent variables, such as initial TCS concentration, pH, removal duration, and temperature, were optimized using the response surface method (RSM). As a result, the maximum TCS removal (97%) was detected at a pH value of 7 and a temperature of 32 °C after 9 days and 12 h of treatment. Gas chromatography-mass spectrometry (GC/MS) analysis showed five intermediate products and a newly proposed pathway for TCS degradation. Finally, the phytotoxicity experiment conducted on Cucumis sativus and Lens culinaris seeds demonstrated an increase in germination power and growth of stems and roots in comparison to untreated water. These results indicate that the final treated water was less toxic.

Ghafouri M ，Pourjafar F ，Ghobadi Nejad Z ，Yaghmaei S ... -  《-》

Treatment of triclosan through enhanced microbial biodegradation.

Triclosan (TCS) is extensively used in healthcare and personal care products as an antibacterial agent. Due to the persistent and toxic nature of TCS, it is not completely degraded in the biological wastewater treatment process. In this research work, identification of TCS degrading bacteria from municipal wastewater sludge and applying the same as bioaugmentation treatment for wastewater have been reported. Based on the 16S rRNA analysis of wastewater sludge, it was found that Providencia rettgeri MB-IIT strain was active and able to grow in higher TCS concentration. The identified bacterial strain was able to use TCS as carbon and energy source for its growth. The biodegradation experiment was optimized for the operational parameters viz. pH (5-10), inoculum size (1-5% (v/v)) and different initial concentration (2, 5, and 10 mg/L) of TCS. During the TCS degradation process, manganese peroxidase (MnP) and laccase (LAC) enzyme activity and specific growth rate of P. rettgeri strain were maximum at pH=7% and 2% (v/v) inoculum size, resulting in 98% of TCS removal efficiency. A total of six intermediate products were identified from the Liquid chromatography-high-resolution mass spectrometry (LC-HRMS) analysis, and the two mechanisms responsible for the degradation of TCS have been elucidated. The study highlights that P. rettgeri MB-IIT strain could be advantageously used to degrade triclosan present in the wastewater.

Balakrishnan P ，Mohan S 《-》

Triclosan Dioxygenase: A Novel Two-component Rieske Nonheme Iron Ring-hydroxylating Dioxygenase Initiates Triclosan Degradation.

Yin Y ，Ren H ，Wu H ，Lu Z ... -  《-》

Characterization of triclosan metabolism in Sphingomonas sp. strain YL-JM2C.

Mulla SI ，Wang H ，Sun Q ，Hu A ，Yu CP ... -  《Scientific Reports》

Experimental investigation for treating ibuprofen and triclosan by biosurfactant from domestic wastewater.

The presence of emerging pollutants of pharmaceutical products and personal care products (PPCPs) in the aquatic environment overspreads the threat on living beings. Bioremediation is a promising option for treating wastewater. In the present study, an experimental investigation was carried out to produce a biosurfactant by Pseudomonas aeruginosa (MTCC 1688) for the removal of Ibuprofen (IBU) and Triclosan (TCS) from domestic wastewater. It was performed in three stages. Firstly, the production and optimization of biosurfactant was carried out to arrive at the best combination of crude sunflower oil, sucrose and ammonium bicarbonate (10%: 5.5 g/L: 1 g/L) to yield effective biosurfactant production (crude biosurfactant) and further extended to achieve critical micelle concentration (CMC) formation by dilution (biosurfactant at 10.5%). The stability of the biosurfactant was also confirmed. Biosurfactant showed a reduction in the surface tension to 41 mN/m with a yield concentration of 11.2 g/L. Secondly, its effectiveness was evaluated for the removal of IBU and TCS from the domestic wastewater collected during the dry and rainy seasons. Complete removal of IBU was achieved at 36 h & 6 h and TCS at 6 h & 1 h by crude biosurfactant and biosurfactant at CMC formation for the dry season sample. IBU removal was achieved in 2 h by both crude and biosurfactant at CMC and no TCS was detected in the rainy season sample. Thirdly, biotransformation intermediates of IBU and TCS formed during the application of the biosurfactant and degradation pathways are proposed based on the Liquid Chromatography-Mass Spectrometry (LC-MS) and it indicates that there is no formation of toxic by-products. Based on the results, it is evident that biosurfactant at CMC has performed better for the removal of IBU and TCS than crude biosurfactants without any formation of toxic intermediates. Hence, this study proved to be an eco-friendly, cost-effective and sustainable treatment option for domestic wastewater treatment.

Jayalatha NA ，Devatha CP 《-》