Combined ecotoxicological effects of different-sized polyethylene microplastics and imidacloprid on the earthworms (Eisenia fetida).

Microplastics (MPs) and pesticides frequently coexist in farmland soil; however, there are relatively few studies on the ecological risk assessment of soil animals attributed to the combined pollution caused by MPs and pesticides. Moreover, the influence of particle size on the combined toxic effects of MPs and pesticides remains poorly understood. In this study, different-sized polyethylene MPs (PE MPs; 10 μm, 500 μm, and 2 mm) were combined with a series of imidacloprid concentrations (IMI; 0.10, 0.50 and 1.00 mg/kg), and earthworms (Eisenia fetida) were exposed to these MP and IMI combinations for 28 d to explore the combined toxic effects and mechanisms. The results showed, compared with IMI or PE MPs exposure alone, the combined exposure of IMI and PE MPs did not substantially increase the acute toxicity of earthworms but significantly inhibited weight increase and induced more serious epidermal damage to earthworms with a size effect; among these 10 μm PE MPs combined with IMI exhibited the strongest toxic effects. In addition, the combined exposure decreased antioxidant enzymes activity and caused oxidative damage in earthworms. Transcriptome results demonstrated most of the treatment combinations affected the ferroptosis pathway, which was further verified by the increase in the total iron content, reactive oxygen species, and malondialdehyde content in earthworms. Combined with the analysis of key signalling pathways, the above results revealed that the combined exposure to IMI and PE MPs showed stronger toxicity to earthworms than exposure to either IMI or MPs alone, which was mediated by the superimposed effect of ferroptosis and oxidative damage. Moreover, the effect was size-dependent, with 10 μm PE MPs combined with IMI exhibiting the strongest toxic effects. This study aimed to provide data to support the ecological risk assessment of soil animals caused by the combined pollution of MPs and coexisting pesticides.

Fu H ，Zhu L ，Mao L ，Zhang L ，Zhang Y ，Chang Y ，Liu X ，Jiang H ... -  《-》

The combined effects of azoxystrobin and different aged polyethylene microplastics on earthworms (Eisenia fetida): A systematic evaluation based on oxidative damage and intestinal function.

Bao X ，Wang Z ，Liu L ，Wang D ，Gu Y ，Chen L ，Chen X ，Meng Z ... -  《-》

Effects of polyethylene microplastics stress on soil physicochemical properties mediated by earthworm Eisenia fetida.

Cheng Y ，Guo Y ，Wang F ，Zhang L ... -  《-》

Ecotoxicological effects of different size ranges of industrial-grade polyethylene and polypropylene microplastics on earthworms Eisenia fetida.

The effects of microplastics (MPs) on terrestrial organisms remain poorly understood, even though soil is an important MPs sink. In this study, the earthworms Eisenia fetida were exposed to 0.25% (w/w) of industrial-grade high-density polyethylene (HDPE, 28-145, 133-415 and 400-1464 μm) and polypropylene (PP, 8-125, 71-383 and 761-1660 μm) MPs in an agricultural soil for 28 d. The results showed that HDPE and PP MPs with different size ranges can be ingested by E. fetida. Exposure to different size ranges of HDPE and PP MPs altered the activities of superoxide dismutase, catalase and glutathione S-transferase and induced an increase in the 8-hydroxy-2'-deoxyguanosine level in E. fetida, suggesting that MPs-induced oxidative stress occurred in E. fetida. A size and type-dependent toxicity of MPs to E. fetida was demonstrated by the integrated biological response index. In addition, to obtain detailed molecular information on the responses of E. fetida to MPs exposure, transcriptomic analysis was conducted for E. fetida from HDPE (28-145 μm) and PP (8-125 μm) treatment groups. Transcriptomic analysis identified 34,937 and 28,494 differentially expressed genes in the HDPE and PP MPs treatments compared with the control, respectively. And, exposure to HDPE and PP MPs significantly disturbed several pathways closely related to neurodegeneration, oxidative stress and inflammatory responses in E. fetida. This study provides important information for the ecological risk assessment of different size ranges and types of industrial-grade MPs.

Li B ，Song W ，Cheng Y ，Zhang K ，Tian H ，Du Z ，Wang J ，Wang J ，Zhang W ，Zhu L ... -  《-》

Influence of aged and pristine polyethylene microplastics on bioavailability of three heavy metals in soil: Toxic effects to earthworms (Eisenia fetida).

Virgin microplastics (MPs) would undergo aging process when entering environment, the adsorption capability of pollutants onto MPs may change during the aging process. To better understand the influence of aged polyethylene microplastics (PE-MP) on the bioavailability of three heavy metals (Zn, Pb, and Cd) in soil, hydrogen peroxide exposure (3% H2O2) and ultraviolet irradiation methods were employed to simulate the aging process. After aging process, different amount (0.1%, 1%, 10%) of PE-MP (pristine or aged) was added into soil to assess the ability of soil (containing PE-MP) adsorbing heavy metal. Moreover, different amount (0.01%, 0.1%, 1%) of PE-MP (pristine or aged) was added into soil to cultivate the earthworms to assess the impact of PE-MP on bioavailability of three heavy metals. Results indicated that the aged and virgin PE-MP had similar capability to adsorb heavy metal, the adsorption ability of Zn2+, Pb2+, and Cd2+ to pristine PE-MP were 2.42, 7.47, and 7.76 mg/g, respectively. The concentration of Zn or Pb in earthworms in treatments of metal +1% PE-MP was slightly higher than that in single metal (Zn or Pb) treatment, moreover, the concentration of Cd in earthworms in treatment of Cd + 1% PE-MP was significantly (p < 0.05) higher than that in single Cd treatment, exhibiting that 1% of PE-MP enhanced the bioavailability of heavy metals in soil. However, heavy metal concentrations in earthworms in treatments of metal + pristine PE-MP showed insignificant (p > 0.05) difference with those in treatments of metal + aged PE-MP, indicating that the aging process in this study did not change the environmental influence of PE-MP on heavy metals bioavailability. Superoxide dismutase activity, reactive oxygen species level, malondialdehyde content, and related gene expression in earthworms showed that PE-MP and heavy metals would bring toxic synergy to earthworms, therefore, the influence of MPs should be comprehensively considered when determining the environmental risk of heavy metals in soil.

Li M ，Jia H ，Gao Q ，Han S ，Yu Y ，Sun L ... -  《-》