Response of soil C-, N-, and P- acquisition enzymes to moisture pulses in desert grassland to shrubland state transition.

Grassland-shrubland state transition causes profound effects on soil nutrients and microorganisms, yet little is known about how these soil characteristics are influenced by rainfall and litter changes during transition. Here, we examined water (high or low moisture pulse) and litter (grass or shrub) effects on these soil characteristics in grassland-shrubland mosaics consisting of desert grassland (DG), grassland edge (GE), shrubland edge (SE), and shrubland (SL) sites. The results showed that the transition of DG-GE-SE-SL significantly reduced soil moisture, total carbon (C), total nitrogen (N), total phosphorus (P), microbial biomass carbon, and microbial biomass nitrogen, revealing evident soil degradation during this transition. After applying water and litter, soil microbial respiration (SMR) and the activities of all enzymes were promoted to varying degrees among the sites. Specifically, SMR was promoted under a low moisture pulse but suppressed under a high moisture pulse along the transition from DG to SL. Two C-acquisition enzymes, cellobiohydrolase and β-1,4-glucosidase, became increasingly active from DG to SL. Another C-acquisition enzyme of β-1,4-xylosidase and an N-acquisition enzyme of leucine aminopeptidase showed the strongest preferences for low moisture pulses in SL. These results indicated that shrub encroachment retained certain microbes with an advanced ability to acquire to C and N from dry and infertile soil in SL. Although a P-acquisition enzyme of alkaline phosphatase showed a decreasing trend along the transition from DG to SL, similar like those C- and N- acquisition enzymes, it was not sensitive to varying moisture levels, suggesting that alkaline phosphatase was affected by other soil physicochemical properties rather than soil moisture. The joint analysis of soil extracellular enzymes and nutrients indicated that microbial biomass carbon played a more important role than other soil characteristics in determining soil extracellular enzyme activities along the transition from DG to SL. Future research on dissecting soil microbial communities is warranted to better understand the microbiological mechanisms behind these phenomena in the shrub encroachment process.

Zhao Y ，Wang H ，Guo T ，Li Z ，Mi W ，Cao Z ... -  《-》

[Responses of soil extracellular enzyme activities to the anthropogenic transition from desert grassland to shrubland in eastern Ningxia, China].

Guo TD ，Yu L ，Sun ZC ，Ma YP ，Zhao YN ，Liang DN ，Li ZL ，Wang HM ... -  《-》

[Responses of soil nitrogen to the transition from desert grassland to shrubland in eastern Ningxia, China].

Li ZL ，Wang HM ，Sun ZC ，Ma YP ，Zhao YN ，Li XY ... -  《-》

Anthropogenic shrub encroachment has accelerated the degradation of desert steppe soil over the past four decades.

Anthropogenic and natural shrub encroachment have similar ecological consequences on native grassland ecosystems. In fact, there is an accelerating trend toward anthropogenic shrub encroachment, as opposed to the century-long process of natural shrub encroachment. However, the soil quality during the transition of anthropogenic shrub encroachment into grasslands remains insufficiently understood. Here, we used a soil quality assessment method that utilized three datasets and two scoring methods to evaluate changes in soil quality during the anthropogenic transition from temperate desert grassland to shrubland. Our findings demonstrated that the soil quality index decreased with increasing shrub cover, from 0.49 in the desert grassland to 0.31 in the shrubland. Our final results revealed a gradual and significant decline of 36.73 % in soil quality during the transition from desert grassland to shrubland. Reduced soil moisture levels, nutrient availability, and microbial activity characterized this decline. Nearly four decades of anthropogenic shrub encroachment have exacerbated soil drought conditions while leading to a decrease in perennial herbaceous plants and an increase in bare ground cover; these factors can explain the observed decline in soil quality. These findings emphasize the importance of considering soil moisture availability and potential thresholds when implementing revegetation strategies in arid and semiarid regions.

Zhao Y ，Wang H ，Li Z ，Lin G ，Fu J ，Li Z ，Zhang Z ，Jiang D ... -  《-》

Biochemical composition and function of subalpine shrubland and meadow soil microbiomes in the Qilian Mountains, Qinghai-Tibetan plateau, China.

Microorganisms participate in the soil biogeochemical cycle. Therefore, investigating variations in microbial biomass, composition, and functions can provide a reference for improving soil ecological quality due to the sensitivity of microorganisms to vegetation coverage changes. However, the differences in soil microorganisms between shrubland and meadow have not been investigated in ecologically vulnerable subalpine areas. This study aimed to investigate the biochemical composition and functions of the soil microbial community under two shrublands and a meadow at high altitudes (3,400-3,550 m). Three sites under two shrublands, Rhododendron thymifolium (RHO) and Potentilla fruticosa (POT), and one meadow dominated by Kobresia myosuroides (MEA), were selected on the southern slope of the Qilian Mountains on the northeastern edge of the Qinghai-Tibetan Plateau, China. Soil physicochemical properties, the microbial community composition expressed by the phospholipid fatty acid (PLFA) biomarker, and enzyme activities were analyzed as well as their relationships. The results showed that water holding capacity and the soil carbon, nitrogen, and potassium content in RHO and POT were higher than those in the MEA. Moreover, the soil active carbon, dissolved organic carbon, total nitrogen, and dissolved total nitrogen content in RHO were higher than those in POT. The abundance of total PLFAs, bacteria, and fungi beneath the shrublands was considerably higher than that in the MEA. The PLFA abundance in RHO was significantly higher than that in POT. The fungal-to-bacterial ratio of RHO and POT was significantly higher than that in the MEA. The activities of β-glucosidase, cellobiohydrolase, and leucine aminopeptidase were the highest in RHO among the three vegetation types, followed by POT and MEA. The redundancy analysis indicated that the biochemical composition of the soil microorganisms and enzyme activities were driven by total nitrogen, dissolved organic carbon, water holding capacity, and soil organic carbon. Therefore, shrublands, which have higher biomass, can improve soil moisture status, increase soil carbon and nitrogen content (especially active carbon and active nitrogen), and further increase the abundance of total PLFAs, bacteria, and fungi. The increase of microbial biomass indirectly enhances the activity of relevant soil enzymes. The variations in PLFA abundance and enzyme activities can be attributed to shrub species, especially evergreen shrubs, which create more favorable conditions for soil microorganisms. This study provides a theoretical basis for investigating the soil biogeochemical cycle and a scientific basis for soil management and vegetation restoration in the subalpine regions.

Fan Q ，Yang Y ，Geng Y ，Wu Y ，Niu Z ... -  《PeerJ》