Penile intraepithelial neoplasia: Distribution of subtypes, HPV genotypes and p16(INK4a) in 84 international cases.

There are few pathologic or molecular studies of penile precancerous lesions, and the majority refers to lesions associated with invasive carcinomas. Penile Intraepithelial Neoplasia (PeIN) is classified in two morphologically and distinctive molecular groups, non-HPV and HPV-related with special subtypes. The primary purpose of this international series was to classify PeIN morphologically, detect HPV genotypes and determine their distribution according to PeIN subtypes. A secondary aim was to evaluate the p16INK4a immunostaining as a possible HPV surrogate for high-risk HPV infection in penile precancerous lesions. Samples consisted of 84 PeIN cases, part of a retrospective cross-sectional analysis of 1095 penile carcinomas designed to estimate the HPV DNA prevalence in penile cancers using PCR and p16INK4a immunostaining. Penile Intraepithelial Neoplasia (PeIN) was classified in HPV-related (basaloid, warty-basaloid, warty, hybrid, and mixed subtypes) and non-HPV-related (differentiated), the former being the most frequent. PeIN subtypes were differentiated (non-HPV-related) and basaloid, warty-basaloid, warty, hybrid and mixed (HPV-related). Basaloid PeIN was the most commonly diagnosed subtype, and HPV16 was the most frequent HPV genotype detected. Warty-basaloid and warty PeIN showed a more heterogeneous genotypic composition. Most HPV genotypes were high-risk but low-risk HPV genotypes were also present in a few cases (4%). A single HPV genotype was detected in 82% of HPV positive cases. In contrast, multiple genotypes were detected in the remaining 18% of cases. The findings in this study support the paradigm that penile in situ neoplasia, like its invasive counterparts, is HPV dependent or independent and has distinctive morphological subtypes readily identified in routine practice. Considering that HPV16 is clearly the predominant type, and that the three available vaccines have HPV16, all of them will be suitable for vaccination programs; the price of the vaccines will be probably the main determinant to choose the vaccine.

Fernández-Nestosa MJ ，Clavero O ，Sánchez DF ，Giannico GA ，Lobatti A ，Cañete-Portillo S ，Velázquez EF ，Alemany L ，Muñoz N ，de San José S ，Bosch FX ，Cubilla AL ... -  《-》

Distinctive association of p16INK4a overexpression with penile intraepithelial neoplasia depicting warty and/or basaloid features: a study of 141 cases evaluating a new nomenclature.

Chaux A ，Pfannl R ，Lloveras B ，Alejo M ，Clavero O ，Lezcano C ，Muñoz N ，de Sanjosé S ，Bosch X ，Hernández-Pérez M ，Velazquez EF ，Cubilla AL ... -  《-》

Human Papillomavirus (HPV) Genotypes in Condylomas, Intraepithelial Neoplasia, and Invasive Carcinoma of the Penis Using Laser Capture Microdissection (LCM)-PCR: A Study of 191 Lesions in 43 Patients.

Fernández-Nestosa MJ ，Guimerà N ，Sanchez DF ，Cañete-Portillo S ，Velazquez EF ，Jenkins D ，Quint W ，Cubilla AL ... -  《-》

Report From the International Society of Urological Pathology (ISUP) Consultation Conference on Molecular Pathology of Urogenital Cancers V: Recommendations on the Use of Immunohistochemical and Molecular Biomarkers in Penile Cancer.

Canete-Portillo S ，Velazquez EF ，Kristiansen G ，Egevad L ，Grignon D ，Chaux A ，Cubilla AL ... -  《-》

Distinctive immunohistochemical profile of penile intraepithelial lesions: a study of 74 cases.

Chaux A ，Pfannl R ，Rodríguez IM ，Barreto JE ，Velazquez EF ，Lezcano C ，Piris A ，Netto GJ ，Cubilla AL ... -  《-》