The Ameliorative Role of Eugenol against Silver Nanoparticles-Induced Hepatotoxicity in Male Wistar Rats.

Silver nanoparticles (AgNPs) utilization is becoming increasingly popular. The existing investigation evaluates the ameliorative impact of eugenol (Eug) against the toxic influences of AgNPs on rats' liver. Sixty adult male rats were enrolled equally into control, Eug (100 mg kg-1 orally), AgNPs-low dose (1 mg kg-1 i.p), AgNPs-high dose (2 mg kg-1 i.p), Eug + AgNPs-low dose (100 mg kg-1 orally + 1 mg kg-1 i.p), and Eug + AgNPs high dose (100 mg kg-1 orally + 2 mg kg-1 i.p). All the groups were treated daily for 30 days, subsequently serum aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), total protein, total albumin, lactate dehydrogenase (LDH), total oxidative capacity (TOC), malondialdehyde (MDA), tumor necrosis factor-alpha (TNF-α), total antioxidant capacity (TAC), and interleukin 6 (IL-6) levels were measured; hepatic tissues superoxide dismutase (SOD), catalase (CAT), reduced glutathione (GSH), and glutathione peroxidase (GPx) levels were evaluated; histopathology and histomorphometry were documented in the liver of all groups; and Bcl-2, P53, Caspase-3, and TNF-α reactive proteins were also immunohistochemically detected. AgNPs significantly triggered oxidative stress in hepatic tissues, characterized by elevated levels of AST, ALT, ALP, LDH, TOC, MDA, TNF-α, and IL-6 correlating with considerable decline in total protein, total albumin, TAC, SOD, CAT, GSH, and GPx. These changes were paralleled with histopathological alterations remarkable by devastation of the ordinary hepatic structure, with decrease in the numbers of normal hepatocytes, elevation in the numbers of necrotic hepatocytes, periportal and centrilobular inflammatory cells, deteriorated Kupffer cells, and dilated/congested central and portal veins. Alongside, a marked diminution in Bcl-2 immunoreactivity and a significant elevation in P53, Caspase-3, and TNF-α immunoreactivities were recorded. Supplementation of AgNPs-treated animals with Eug reversed most of the biochemical, histopathological, and immunohistochemical changes. This study proposed that Eug has an ameliorative effect against AgNPs-induced hepatotoxicity.

Yousef HN ，Ibraheim SS ，Ramadan RA ，Aboelwafa HR ... -  《-》

Histomorphological changes and molecular mechanisms underlying the ameliorative effect of resveratrol on the liver of silver nanoparticles-exposed rats.

Abdelrahman SA ，Mahmoud AA ，Abdelrahman AA ，Samy W ，Zaid Hassen Saleh E ... -  《-》

Modulation Effects of Eugenol on Nephrotoxicity Triggered by Silver Nanoparticles in Adult Rats.

Aboelwafa HR ，Ramadan RA ，Ibraheim SS ，Yousef HN ... -  《Biology-Basel》

Protective effect of eugenol on hepatic inflammation and oxidative stress induced by cadmium in male rats.

Cadmium is one of the most toxic heavy metals. The prolonged exposure of it can lead to severe alterations and damage in different tissues including blood, liver, kidney and brain. Eugenol, a phenolic compound, is present in various aromatic plants. It acts as a natural antioxidant and anti-inflammatory agent. The aim of this study was to investigate whether the treatment of eugenol is beneficial against the hepatic oxidative stress and inflammation induced by Cd. To study the effect of eugenol in reversal of Cd toxicity, 24 albino rats were equally divided into four different groups: G1 Control (saline), G2 Eugenol (3 mg kg-1), G3 CdCl2 (5 mg kg-1) and G4 CdCl2 + Eugenol (5 mg kg-1 + 3 mg kg-1). All the groups were treated with gavage orally for the period of 21 days. After this treatment period, rats were sacrificed and liver tissues were removed. The hepatic antioxidant status was evaluated by measuring the activities of SOD, Catalase and GST enzymes. The reduced glutathione, lipid peroxidation, protein carbonyl oxidation (PCO) and thiol contents were measured in hepatic tissues. The activities of liver marker enzymes such as ALT, AST, GGT, ALP, TP, albumin, Bilirubin content and LDH were determined to assess the hepatic damage in different groups. Cd induced hepatic inflammation was determined by evaluating the levels of TNF-a, IL-6 and NO. Oral intoxication of Cd for 21 days significantly elevated the level of hepatic markers including activities of LDH, GGT, ALP, ALT, AST and Bilirubin level. The albumin content, reduced GSH level, and activities of antioxidant enzymes were significantly reduced in Cd treated group. The levels of inflammatory markers were significantly elevated in Cd treated group. The eugenol treatment was very effective and it significantly reversed the Cd induced biochemical alterations almost similar to that of control. The results demonstrated that the eugenol possessed very strong anti-oxidative and anti-inflammatory potential. The co-treatment of eugenol with Cd exhibited protective potential of eugenol against Cd induced toxicity. Eugenol was able to improve the cellular redox system in rats treated with Cd.

Kumar A ，Siddiqi NJ ，Alrashood ST ，Khan HA ，Dubey A ，Sharma B ... -  《-》

Protective effects of apigenin on altered lipid peroxidation, inflammation, and antioxidant factors in methotrexate-induced hepatotoxicity.

Goudarzi M ，Kalantar M ，Sadeghi E ，Karamallah MH ，Kalantar H ... -  《-》