Does environmental metal pollution affect bird morphometry? A case study on the tree sparrow Passer montanus.

Morphological characteristics are the leading indicators of the health status of birds. To explore the effects of heavy metals on bird morphometry in natural populations, tree sparrows (Passer montanus) were studied in a polluted site [Baiyin (BY)] and a relatively unpolluted site [Liujiaxia (LJX)]. This study aimed to examine whether morphological variables, the fluctuating asymmetry (FA) of the wing, tarsus, and toe length, were associated with heavy metals (Cu, Zn, Pb, and Cd) and Ca levels in different tissues and feces of adults and nestlings. Results showed that adults collected from BY contained relatively higher heavy metal concentrations and lower Ca concentrations in different tissues than those from LJX. Smaller body sizes and higher FA levels of adults and nestlings were found in BY than in LJX. Although higher heavy metal concentrations in some tissues were associated with smaller morphological characteristics of adults, the effects were not obvious in nestlings. The most correlated heavy metal with as many characteristics was heavy metal in primary feather in both sites, and the most affected characteristic was body mass in BY. The FA values of adults and nestlings in BY were positively affected by heavy metal concentrations in different tissues and feces. The growth rate of wing and tarsus length of nestlings in BY were negatively affected by the FA values of wing and tarsus length, respectively. Taken together, environmental metal pollution might affect the morphological characteristics of tree sparrows. These findings suggest that the morphological characteristics of tree sparrows, especially FA, can be used as indicators of metal pollution, underscoring the importance of measuring morphological characteristics in avian ecotoxicology field studies.

Ding J ，Yang W ，Wang S ，Zhang H ，Zhang Y ... -  《-》

Tissue distribution and association of heavy metal accumulation in a free-living resident passerine bird tree sparrow Passer montanus.

Passerine birds have been increasingly used as effective sentinels of ecosystem contamination. They can provide direct evidence of the bioavailability and accumulation of heavy metal elements in the environment. In this study, the bioaccumulation of four heavy metals (Cu, Zn, Pb, and Cd) and Ca in different organs and tissues (feathers, internal organs, skeletons, and muscles) of an urban bird, tree sparrow (Passer montanus), collected from a polluted site [Baiyin (BY)] and a relatively unpolluted site [Liujiaxia (LJX)], and their associations were investigated. There were significantly higher and lower concentrations of heavy metals and Ca, respectively, in different organs and tissues of sparrows in BY than those in LJX. However, except for Pb, the heavy metal levels were below the threshold of sublethal effects. Age-dependent variations in metals were quantified, and it was found that adult bird contained higher concentration of different organs and tissue metals, except for feathers, compared with nestlings and juveniles. The tissue distribution of heavy metals in sparrows of different ages and sex was similar in the two study sites, and heavy metal elements were mainly accumulated in the feathers. This study further investigated the correlation between heavy metals in different organs and tissues and found that the correlations between them were strong in nestlings and adults but weak in juveniles. In addition, Pb and Cd in internal organs, skeletons, and muscles of young sparrows can be estimated using feathers, whereas Cu and Zn were found in adult sparrows. Altogether, our results suggest that tree sparrows will serve as valuable biomonitors of environmental heavy metal pollution and will underscore the importance of tissue types in avian ecotoxicology field studies.

Ding J ，Wang S ，Yang W ，Zhang H ，Yu F ，Zhang Y ... -  《-》

Effects of environmental metal pollution on reproduction of a free-living resident songbird, the tree sparrow (Passer montanus).

Anthropogenic metal pollution is known to adversely affect bird reproduction; however, few systematic studies are available on the effects of metal pollution on breeding performance and parental investment in a common resident songbird, the tree sparrow (Passer montanus). We conducted this study in two sites, a long-term heavy metal polluted site (Baiyin [BY]) and a relatively unpolluted site at approximately 110 km distance (Liujiaxia [LJX]), to assess the potential effects of environmental metal contamination on breeding parameters (clutch size, hatching success, fledging success, and growth of nestlings) and parental investment. The results showed smaller clutch size, lower fledging success, and differences in incubation behaviors of tree sparrows in BY than in LJX. Although there was no difference in parental body condition (residual body mass) between the two study sites, the parents responded differently with respect to reproduction due to varying metal levels in their habitats and bodies. Higher Cd levels in the primary feathers of females in BY were associated with lower clutch sizes. Parental investment including incubation duration and feeding rates showed no significant difference between the two sites during the incubation and nestling periods, but the frequencies of incubation visits were higher in BY. Parental behavior during the incubation period was also negatively affected by the parental Pb and Cd levels. Although the nestling growth patterns were relatively similar between the two sites, the nestlings were smaller, had lower body weight, and fledged later and fledging rate was also lower in BY than in LJX. Metal concentrations were higher in nestling organs and feces in BY. Taken together, metal pollution might adversely affect nestling growth condition. Our results suggest a negative response in the reproduction of tree sparrows to long-term environmental metal pollution.

Ding J ，Yang W ，Wang S ，Zhang H ，Yang Y ，Bao X ，Zhang Y ... -  《-》

Variations in tree sparrow (Passer montanus) egg characteristics under environmental metal pollution.

Environmental metal pollution is known to adversely affect bird reproduction, for which the variations of egg characteristics are considered very important. Our study explored whether variations in egg characteristics, such as egg volume, eggshell spotting pattern, eggshell coloration, and eggshell thickness, were correlated with heavy metal levels (Cu, Zn, Pb, and Cd) and Ca levels in the eggshells of tree sparrows (Passer montanus), a widespread passerine species. Eggs were collected from a long-term heavy metal polluted area (Baiyin, BY, northwest China) and a relatively unpolluted area (Liujiaxia, LJX, northwest China). Our results showed that the embryonated (eggshell: Cu: p = 0.003, Pb: p = 0.002) and non-embryonated (egg contents: Pb: p = 0.044, Ca: p = 0.045) eggs collected from BY contained relatively higher metal concentrations than those from LJX. Eggs from BY were smaller in volume (p < 0.01) and thinner in eggshell thickness (p < 0.01) than those from LJX. Mean egg volume increased with clutch size in BY (p = 0.017), which was also accompanied by an increase in the within-clutch coefficient of variation (CV) for egg volume (p = 0.045). Clutches with a higher CV for egg volume tended to contain higher concentrations of Zn and Pb (Zn: p = 0.084; Pb: p = 0.081) in the eggshells from BY. No differences were found in the eggshell spotting coverage ratio of eggs; however, eggshells were much darker in BY than in LJX. A more aggregated eggshell spotting distribution indicated higher eggshell Zn and Pb levels (BY: Zn: p = 0.040, Pb: p = 0.076; LJX: Pb: p = 0.066). The results demonstrate that the egg characteristics of tree sparrows can be used as indicators of metal pollution, especially for the within-clutch CV for egg volume, eggshell spotting pattern and eggshell coloration.

Ding J ，Yang W ，Yang Y ，Ai S ，Bai X ，Zhang Y ... -  《-》

Variation in genetic diversity of tree sparrow (Passer montanus) population in long-term environmental heavy metal polluted areas.

Genetic diversity is the bedrock of evolution. The "Genetic Erosion" hypothesis posits that environmental pollution could cause reduced genetic diversity. To explore the effects of heavy metal pollution on genetic diversity in natural populations, we selected an area with more than sixty years of heavy metal contamination (Baiyin, BY) and a relatively unpolluted one (Liujiaxia, LJX), and tree sparrow (Passer montanus) as study models. Five tree sparrow populations were sampled in BY at sites differing in heavy metal pollution level. Lower genetic diversity based on seven microsatellite loci was observed in the five tree sparrow populations from BY compared with those from LJX. Analysis of molecular variance indicated no significant genetic differentiation between BY and LJX. However, the observed heterozygosity and allelic richness were negatively correlated to the lead and cadmium concentrations in the primary feathers of tree sparrow. Our results indicated the genetic diversity might have a negative response to long-term environmental heavy metal pollution in tree sparrow, supporting the "Genetic Erosion" hypothesis. Therefore, the findings shed lights on the possible effects of heavy metal pollution on genetic diversity of wild bird populations.

Yang W ，Ding J ，Wang S ，Yang Y ，Song G ，Zhang Y ... -  《-》