A longitudinal SARS-CoV-2 biorepository for COVID-19 survivors with and without post-acute sequelae.

SARS-CoV-2 has swept across the globe, causing millions of deaths worldwide. Though most survive, many experience symptoms of COVID-19 for months after acute infection. Successful prevention and treatment of acute COVID-19 infection and its associated sequelae is dependent on in-depth knowledge of viral pathology across the spectrum of patient phenotypes and physiologic responses. Longitudinal biobanking provides a valuable resource of clinically integrated, easily accessed, and quality-controlled samples for researchers to study differential multi-organ system responses to SARS-CoV-2 infection, post-acute sequelae of COVID-19 (PASC), and vaccination. Adults with a history of a positive SARS-CoV-2 nasopharyngeal PCR are actively recruited from the community or hospital settings to enroll in the Northern Colorado SARS-CoV-2 Biorepository (NoCo-COBIO). Blood, saliva, stool, nasopharyngeal specimens, and extensive clinical and demographic data are collected at 4 time points over 6 months. Patients are assessed for PASC during longitudinal follow-up by physician led symptom questionnaires and physical exams. This clinical trial registration is NCT04603677 . We have enrolled and collected samples from 119 adults since July 2020, with 66% follow-up rate. Forty-nine percent of participants assessed with a symptom surveillance questionnaire (N = 37 of 75) had PASC at any time during follow-up (up to 8 months post infection). Ninety-three percent of hospitalized participants developed PASC, while 23% of those not requiring hospitalization developed PASC. At 90-174 days post SARS-CoV-2 diagnosis, 67% of all participants had persistent symptoms (N = 37 of 55), and 85% percent of participants who required hospitalization during initial infection (N = 20) still had symptoms. The most common symptoms reported after 15 days of infection were fatigue, loss of smell, loss of taste, exercise intolerance, and cognitive dysfunction. Patients who were hospitalized for COVID-19 were significantly more likely to have PASC than those not requiring hospitalization, however 23% of patients who were not hospitalized also developed PASC. This patient-matched, multi-matrix, longitudinal biorepository from COVID-19 survivors with and without PASC will allow for current and future research to better understand the pathophysiology of disease and to identify targeted interventions to reduce risk for PASC. Registered 27 October 2020 - Retrospectively registered, https://clinicaltrials.gov/ct2/show/NCT04603677 .

LaVergne SM ，Stromberg S ，Baxter BA ，Webb TL ，Dutt TS ，Berry K ，Tipton M ，Haberman J ，Massey BR ，McFann K ，Alnachoukati O ，Zier L ，Heacock T ，Ebel GD ，Henao-Tamayo M ，Dunn J ，Ryan EP ... -  《BMC INFECTIOUS DISEASES》

Quality of Life (QoL) Is Reduced in Those with Severe COVID-19 Disease, Post-Acute Sequelae of COVID-19, and Hospitalization in United States Adults from Northern Colorado.

McFann K ，Baxter BA ，LaVergne SM ，Stromberg S ，Berry K ，Tipton M ，Haberman J ，Ladd J ，Webb TL ，Dunn JA ，Ryan EP ... -  《-》

A Longitudinal Study of COVID-19 Sequelae and Immunity: Baseline Findings.

Sneller MC ，Liang CJ ，Marques AR ，Chung JY ，Shanbhag SM ，Fontana JR ，Raza H ，Okeke O ，Dewar RL ，Higgins BP ，Tolstenko K ，Kwan RW ，Gittens KR ，Seamon CA ，McCormack G ，Shaw JS ，Okpali GM ，Law M ，Trihemasava K ，Kennedy BD ，Shi V ，Justement JS ，Buckner CM ，Blazkova J ，Moir S ，Chun TW ，Lane HC ... -  《-》

Correlation between 25-hydroxyvitamin D/D3 Deficiency and COVID-19 Disease Severity in Adults from Northern Colorado.

Baxter BA ，Ryan MG ，LaVergne SM ，Stromberg S ，Berry K ，Tipton M ，Natter N ，Nudell N ，McFann K ，Dunn J ，Webb TL ，Armstrong M ，Reisdorph N ，Ryan EP ... -  《Nutrients》

Testing the efficacy and safety of BIO101, for the prevention of respiratory deterioration, in patients with COVID-19 pneumonia (COVA study): a structured summary of a study protocol for a randomised controlled trial.

Dioh W ，Chabane M ，Tourette C ，Azbekyan A ，Morelot-Panzini C ，Hajjar LA ，Lins M ，Nair GB ，Whitehouse T ，Mariani J ，Latil M ，Camelo S ，Lafont R ，Dilda PJ ，Veillet S ，Agus S ... -  《Trials》