Nonionic surfactants based hydrophobic deep eutectic solvents for liquid-liquid microextraction of Sudan dyes in tomato chili sauces.

A new type of high-density hydrophobic deep eutectic solvents (DESs) were synthesized with nonionic surfactants as hydrogen bond acceptors and hexafluoroisopropanol (HFIP) as hydrogen bond donor. Brij-35 was selected as the optimal nonionic surfactant for the preparation of Brij-35-HFIP-DES (molar ratio 1:20). A vortex-assisted DES-based liquid-liquid microextraction method was proposed for determination of Sudan dyes in tomato chili sauces. The whole pretreatment process only needs 5 min and 1.1 mL of organic solvent. The method with HPLC-DAD shows high efficiency (enrichment factors 89-176 and extraction rates 61.0-74.6%) and good performance with linearity (R ≥ 0.9997) in 0.04-2 μg g-1 range, detection limits of 0.0045-0.0118 μg g-1, recoveries of 91.6-104.5% and intra-/inter-day precision below 8.0%. A "DES in water in DES" aggregate microstructure was observed in DES-rich phase. The proposed method is simple, quick, eco-friendly, and suits for the efficient extraction and accurate determination of Sudan dyes in tomato chili sauces.

Chen J ，Li X ，Huang A ，Deng W ，Xiao Y ... -  《-》

Preparation of new hydrophobic deep eutectic solvents and their application in dispersive liquid-liquid microextraction of Sudan dyes from food samples.

Ge D ，Shan Z ，Pang T ，Lu X ，Wang B ... -  《-》

Deep eutectic solvents with low viscosity for automation of liquid-phase microextraction based on lab-in-syringe system: Separation of Sudan dyes.

In the work limitations of deep eutectic solvents in the flow-based analysis are discussed. Deep eutectic solvents based on terpenes and fatty acids with low viscosity were studied as extraction solvents for liquid-liquid microextraction into a lab-in-syringe system for the first time. As a result an automated deep eutectic solvent-based microextraction approach was proposed. The procedure involved aspiration of deep eutectic solvent (based on terpene and fatty acid) and aqueous sample solution followed by phases mixing by a magnetic stirrer inside a syringe of flow system. After phase separation the extract phase was transferred from the syringe into a vial followed by analysis by a high-performance liquid chromatography with diode-array detection. The determination of Sudan I, Sudan II and Sudan III in chili-based sauces was considered as an analytical task. The mass-transfer intensification performed by the magnetic stirring inside the syringe allowed to perform fast (2 min) and efficient (extraction recoveries 87-95%) extraction. The limits of detection, calculated from a blank test based on 3σ, were from 0.003 to 0.005 mg kg-1, RSD was <9%. The microextraction procedure did not involve the use of hazardous organic solvents, only 100 μL of natural deep eutectic solvent was required for dyes preconcentration.

Shishov A ，Pochivalov A ，Dubrovsky I ，Bulatov A ... -  《-》

Determination of fifteen illegal colorants in traditional Chinese medicines by two hydrophobic DES-based microextraction methods coupled with an HPLC-DAD.

The dyeing and adulteration of traditional Chinese medicines (TCMs) are continuously updated. Valuable analytical methods for the daily inspection of illegal colorant additives in TCMs and the preparations are in demand. Two deep eutectic solvent (DES)-based vortex-assisted liquid-liquid microextraction (VA-LLME) and ultrasonic-assisted solid-liquid microextraction (UA-SLME) were developed for the sample pretreatment of ten water-soluble colorants and five water-insoluble colorants, respectively, followed by an HPLC-DAD detection. Fifteen colorants were analyzed at four detection wavelengths within 40 min of gradient elution. The optimal DES of VA-LLME and UA-SLME were screened from 23 homemade DESs. The factors affecting the extraction efficiency of VA-LLME and UA-SLME were optimized systematically. Under the optimal conditions, ten water-soluble colorants analyzed by DES-based VA-LLME-HPLC-DAD showed good linearity (R ≥ 0.9995) within the optimal linear range. The LODs and LOQs were 0.2-1.0 μg g-1 and 0. 5-5.0 μg g-1, respectively. The recoveries of spiked samples were 80.2%-104.7 %, with RSDs ≤ 4.39 %. Five water-insoluble colorants of Sudan I‒IV and Sudan 7B analyzed by DES-based UA-SLME-HPLC-DAD showed good linearity (R ≥ 0.9995) within the optimal linear range. The LODs and LOQs were 0.8-8.0 μg g-1 and 4.0-40.0 μg g-1, respectively. The recoveries of spiked samples were 94.2%-103.1 %, with RSDs ≤ 4.81 %. The proposed DES-based VA-LLME-HPLC-DAD was successfully applied to analyze six water-soluble yellow colorants in Cuscutae Semen, salted Cuscutae Semen, and four water-soluble red colorants in Schisandrae Chinensis Fructus. The proposed DES-based UA-SLME-HPLC-DAD was successfully applied to analyze five water-insoluble red colorants in Dieda pills. The study provides analytical method options for routine tests of water-soluble, water-insoluble, or both water-soluble/-insoluble illegal colorant additives in herbal medical materials and preparations by the relevant proposed DES-based sample pretreatment method or a combination of the two proposed DES-based methods.

Peng J ，Hassan FA ，Wu J ，Xiong C ... -  《-》

Hexafluoroisopropanol-based hydrophobic deep eutectic solvents for dispersive liquid-liquid microextraction of pyrethroids in tea beverages and fruit juices.

A series of new hydrophobic deep eutectic solvents (DESs), which are liquid at room temperature and have high density (>1.4 g mL-1), were synthesized using hexafluoroisopropanol (HFIP) as hydrogen-bond donor and l-carnitine/betaine as hydrogen-bond acceptor. Then these hydrophobic DESs were used as extraction solvents to establish dispersive liquid-liquid microextraction (DLLME) method for extraction of pyrethroids. The DES extraction phase was in the bottom after DLLME, being easy to be collected for analysis. After optimization by one-variable-at-a-time and response surface methodology, the enrichment factors of 265-360 were achieved for five pyrethroids. The proposed DLLME method coupled with HPLC has good performance: linear ranges of 0.25/0.5/1-100/200/400 ng/mL (r ≥ 0.9990), limits of detection of 0.06-0.17 ng mL-1, relative recoveries of 85.1-109.4%, intra-day and inter-day RSDs below 7.5%. The novel DLLME method is simple, rapid, highly efficient and eco-friendly for extraction of pyrethroids in real tea beverages and fruit juices.

Deng W ，Yu L ，Li X ，Chen J ，Wang X ，Deng Z ，Xiao Y ... -  《-》