Effect of operational strategies on microbial water quality in small scale intermittent water supply systems: The case of Moamba, Mozambique.

Intermittent drinking water supply affects the health of over 300 million people globally. In Mozambique, it is largely practiced in cities and small towns. This results in frequent microbial contamination of the supplied drinking water posing a health risk to consumers. In Moamba, a small town in Southern Mozambique with 2,500 water connections, the impact of changes in operational strategies, namely increased chlorine dosage, increased supply duration and first-flush, on the microbial water quality was studied to determine best practices. To that aim, water quality monitoring was enhanced to provide sufficient data on the microbial contamination from 452 samples under the different strategies. The water at the outlet of the water treatment plant during all strategies was free of E. coli complying to the national standards. However, E. coli could be detected at household level. By increasing the chlorine dosage, the number of samples that showed E. coli absence increased at the two sampling locations in the distribution network: in Cimento from 72% to 83% and in Matadouro from 52% to 86%. Modifying the number and duration of supply cycles showed a different impact on the water quality at both locations in the distribution network. A positive effect was shown in Cimento, where the mean concentrations decreased slightly from 0.54 to 0.23 CFU/100 mL and 16.7 to 7.3 CFU/100 mL for E. coli and total coliforms respectively. The percentage of samples positive for bacteria was, however, similar. In contrast, a negative effect was shown in Matadouro where the percentage of positive samples increased and the mean bacterial concentrations increased slightly: E. coli from 0.9 to 1.5 CFU/100 mL and total coliforms 17.6 to 23.0 CFU/100 mL. Enhanced water quality monitoring improved operational strategies safeguarding the microbial water quality. The E. coli contamination of the drinking water at household level could point at recontamination in the distribution or unsafe hygienic practices at household level. Presence of faecal contamination at household level indicates potential presence of pathogens posing a health risk to consumers. Increasing chlorine dosage ensured good microbiological drinking water quality but changing the number of supply cycles had no such effect.

van den Berg H ，Quaye MN ，Nguluve E ，Schijven J ，Ferrero G ... -  《-》

Occurrence of waterborne pathogens and antibiotic resistance in water supply systems in a small town in Mozambique.

. Microbiological quality of drinking water supplied in Moamba, a small town in southern Mozambique, was assessed by collecting and analyzing 91 water sample from 5 sampling sites: raw or inlet water, treated water and 3 household taps along the water distribution system. The presence of Escherichia coli as indicator fecal contamination, three bacterial pathogens, Vibrio cholerae, Salmonella and Campylobacter spp., and Cefotaximee resistant E. coli as antibiotic resistance determinant, was assessed. . The results showed fecal contamination in all types of water samples: E. coli was found in 100% of inlet water samples, in 21% of treated water samples, and in 22% of tap water samples. No Salmonella spp. was detected during the study. The presence of V. cholerae was detected in 42% of all water samples tested: 100% of inlet water samples, in 16% of treated water samples, and in 23% household tap water samples. All V. cholerae confirmed isolates where genotyped by PCR as non-O1/non-O139; however, 9 isolates showed the presence of the genes encoding for cholera toxin. The presence of Campylobacter spp. was detected in 36% of the water samples tested: in 95% of inlet water samples, in 10% of treated water samples and in 23% household tap water samples. Cefotaxime resistant E. coli was detected in 63% of inlet water, 16% of treated water, and in 9% of tap water samples, these isolates were also resistant to multiple other antibiotics: ampicillin, streptomycin, tetracycline chloramphenicol. All 70 V. cholerae non-O1/non-O139 confirmed isolated were resistant to ampicillin, 51% to streptomycin, 13% to gentamycin, and 1 isolate was resistant to tetracycline; 13% showed a multi-drug resistant profile, being resistant to at least three antibiotics. . The presence of fecal contamination and pathogens in the water treatment system and household taps in Moamba indicates a health risk for the population. This burden increases by the presence of bacterial pathogens showing multidrug resistance.

Taviani E ，van den Berg H ，Nhassengo F ，Nguluve E ，Paulo J ，Pedro O ，Ferrero G ... -  《BMC MICROBIOLOGY》

Comparing microbial water quality in an intermittent and continuous piped water supply.

Supplying piped water intermittently is a common practice throughout the world that increases the risk of microbial contamination through multiple mechanisms. Converting an intermittent supply to a continuous supply has the potential to improve the quality of water delivered to consumers. To understand the effects of this upgrade on water quality, we tested samples from reservoirs, consumer taps, and drinking water provided by households (e.g. from storage containers) from an intermittent and continuous supply in Hubli-Dharwad, India, over one year. Water samples were tested for total coliform, Escherichia coli, turbidity, free chlorine, and combined chlorine. While water quality was similar at service reservoirs supplying the continuous and intermittent sections of the network, indicator bacteria were detected more frequently and at higher concentrations in samples from taps supplied intermittently compared to those supplied continuously (p < 0.01). Detection of E. coli was rare in continuous supply, with 0.7% of tap samples positive compared to 31.7% of intermittent water supply tap samples positive for E. coli. In samples from both continuously and intermittently supplied taps, higher concentrations of total coliform were measured after rainfall events. While source water quality declined slightly during the rainy season, only tap water from intermittent supply had significantly more indicator bacteria throughout the rainy season compared to the dry season. Drinking water samples provided by households in both continuous and intermittent supplies had higher concentrations of indicator bacteria than samples collected directly from taps. Most households with continuous supply continued to store water for drinking, resulting in re-contamination, which may reduce the benefits to water quality of converting to continuous supply.

Kumpel E ，Nelson KL 《-》

Microbial quality of water supply to an urban community in Trinidad.

Agard L ，Alexander C ，Green S ，Jackson M ，Patel S ，Adesiyun A ... -  《JOURNAL OF FOOD PROTECTION》

Assessment of Bacteriological and Physicochemical Quality of Drinking Water from Source to Household Tap Connection in Nekemte, Oromia, Ethiopia.

Duressa G ，Assefa F ，Jida M 《-》