SARS-CoV-2 in saliva, oropharyngeal and nasopharyngeal specimens.

The reference test to evaluate patients with suspected respiratory virus infection is a real-time reverse transcription-polymerase chain reaction (RT-PCR) from a nasopharyngeal swab (NPS). However, other specimen collection methods such as an oropharyngeal swab (OPS) or saliva specimen are also used for SARS-CoV-2 testing during the ongoing COVID-19 pandemic. However, it remains unclear if rates of SARS-CoV-2 detection differ between sampling methods. This study will compare the rates of SARS-CoV-2 detection by saliva, OPS, and NPS sampling in a public setting. Individuals referred for outpatient SARS-CoV-2 testing will be invited to participate in a prospective clinical study. They will have saliva, OPS and NPS specimens collected that will be analysed separately for SARS-CoV-2 RNA by RT-PCR. The rate of SARS-CoV-2 detection in saliva, OPS and NPS will be compared using a logistic regression mixed-effect model analysis. A sample of 19,110 participants is required at an expected 1.5% test-positive rate in order to detect a 25.6% difference. The total sample size will be adjusted as the test-positive rate changes. This study will provide evidence for the optimal site of specimen collection to detect SARS-CoV-2. The results may help guide the health authorities. This is an investigator-initiated trial based on an unrestricted grant from the Novo Nordisk Foundation and the Aage og Johanne Louis-Hansens Fond. The foundations have had no say in the decisions on study design or reporting. ClinicalTrials.gov (ID: NCT04715607).

Todsen T ，Tolsgaard M ，Folke F ，Jakobsen KK ，Ersbøll AK ，Benfield T ，von Buchwald C ，Kirkby N ... -  《Danish Medical Journal》

Sensitivity of nasopharyngeal, oropharyngeal, and nasal wash specimens for SARS-CoV-2 detection in the setting of sampling device shortage.

Calame A ，Mazza L ，Renzoni A ，Kaiser L ，Schibler M ... -  《-》

Are Posterior Oropharyngeal Saliva Specimens an Acceptable Alternative to Nasopharyngeal Sampling for the Monitoring of SARS-CoV-2 in Primary-Care Settings?

Masse S ，Bonnet C ，Vilcu AM ，Benamar H ，Swital M ，van der Werf S ，Carrat F ，Hanslik T ，Blanchon T ，Falchi A ... -  《Viruses-Basel》

Diagnostic Performance of Self-Collected Saliva Versus Nasopharyngeal Swab for the Molecular Detection of SARS-CoV-2 in the Clinical Setting.

Uddin MKM ，Shirin T ，Hossain ME ，Alam AN ，Ami JQ ，Hasan R ，Miah M ，Shaly NJ ，Ahmed S ，Rahman SMM ，Rahman M ，Banu S ... -  《Microbiology Spectrum》

Detection of SARS-CoV-2 from Saliva as Compared to Nasopharyngeal Swabs in Outpatients.

Kandel C ，Zheng J ，McCready J ，Serbanescu MA ，Racher H ，Desaulnier M ，Powis JE ，Vojdani K ，Finlay L ，Sheldrake E ，Vermeiren C ，Katz K ，McGeer A ，Kozak R ，Goneau LW ... -  《Viruses-Basel》