W2476 represses TXNIP transcription via dephosphorylation of FOXO1 at Ser319.

Thioredoxin-interacting protein (TXNIP) overexpression is implicated in the pathogenesis of type 2 diabetes. Previous studies have shown that a small molecule compound (W2476) was able to improve β-cell dysfunction and exert therapeutic effects in diabetic mice via repression of TXNIP signaling pathway. The impact of W2476 on TXNIP transcription was thus investigated using the chromatin immunoprecipitation method. It was found that W2476 promotes competitive binding of forkhead box O1 transcription factor (FOXO1) to the carbohydrate response element (ChoRE) sequence associated with ChoRE-binding protein (ChREBP)/Mlx interacting protein-like(Mlx) complexes. This interaction hinders the attachment of histone acetyltransferase p300 and reduces histone H4 acetylation on the TXNIP promoter, leading to decreasing TXNIP transcription.

Zhong L ，Liu Q ，Liu Q ，Zhang S ，Cao Y ，Yang D ，Wang MW ... -  《-》

FOXO1 competes with carbohydrate response element-binding protein (ChREBP) and inhibits thioredoxin-interacting protein (TXNIP) transcription in pancreatic beta cells.

Kibbe C ，Chen J ，Xu G ，Jing G ，Shalev A ... -  《-》

W2476 ameliorates β-cell dysfunction and exerts therapeutic effects in mouse models of diabetes via modulation of the thioredoxin-interacting protein signaling pathway.

Li T ，Lin GY ，Zhong L ，Zhou Y ，Wang J ，Zhu Y ，Feng Y ，Cai XQ ，Liu Q ，Nosjean O ，Boutin JA ，Renard P ，Yang DH ，Wang MW ... -  《-》

Glucose-stimulated expression of Txnip is mediated by carbohydrate response element-binding protein, p300, and histone H4 acetylation in pancreatic beta cells.

Cha-Molstad H ，Saxena G ，Chen J ，Shalev A ... -  《-》

New Insight Into Metformin Action: Regulation of ChREBP and FOXO1 Activities in Endothelial Cells.

Li X ，Kover KL ，Heruth DP ，Watkins DJ ，Moore WV ，Jackson K ，Zang M ，Clements MA ，Yan Y ... -  《-》