Development and validation of a CT-based nomogram for preoperative prediction of clear cell renal cell carcinoma grades.

Nuclear grades are proved to be one of the most significant prognostic factors for clear cell renal cell carcinoma (ccRCC). Radiomics nomogram is a widely used noninvasive tool that could predict tumor phenotypes. In this study, we performed radiomics analysis to develop and validate a CT-based nomogram for the preoperative prediction of nuclear grades in ccRCC. CT images and clinical data of 258 ccRCC patients were retrieved from the Cancer Imaging Archive (TCIA). Radiomics features were extracted from arterial-phase CT images using 3D Slicer software. LASSO regression model was performed to develop a radiomics signature in the training set (n = 143). A radiomics nomogram was constructed combining radiomics signature and selected clinical predictors. Receiver operating characteristic (ROC) curve and calibration curve were used to determine the performance of the radiomics nomogram in the training and validation set (n = 115). Decision curve analysis was used to assess the clinical usefulness of the CT-based nomogram. One thousand three hundred sixteen radiomics features were extracted from arterial-phase CT images. A radiomics signature, consisting of 20 features, was developed and showed a favorable performance in discriminating nuclear grades with an area under the curve (AUC) of 0.914 and 0.846 in the training and validation set, respectively. The CT-based nomogram, including the radiomics signature and the CT-determined T stage, achieved good calibration and discrimination in the training set (AUC, 0.929; 95% CI, 0.886-0.972) and validation set (AUC, 0.876; 95% CI, 0.812-0.939). Decision curve analysis demonstrated the clinical usefulness of the CT-based nomogram. The noninvasive CT-based nomogram, including radiomics signature and CT-determined T stage, could improve the accuracy of preoperative grading of ccRCC and provide individualized treatment for ccRCC patients. • Contrast-enhanced CT may help in preoperative grading of ccRCC. • The CT-based nomogram incorporated a radiomics signature and CT-determined T stage could preoperatively predict ccRCC grades. • The CT-based nomogram has the potential to improve individualized treatment and assist clinical decision making of ccRCC patients.

Zheng Z ，Chen Z ，Xie Y ，Zhong Q ，Xie W ... -  《-》

A CT-based radiomics nomogram for differentiation of renal angiomyolipoma without visible fat from homogeneous clear cell renal cell carcinoma.

Nie P ，Yang G ，Wang Z ，Yan L ，Miao W ，Hao D ，Wu J ，Zhao Y ，Gong A ，Cui J ，Jia Y ，Niu H ... -  《-》

Computed tomography-based radiomics prediction of CTLA4 expression and prognosis in clear cell renal cell carcinoma.

To predict CTLA4 expression levels and prognosis of clear cell renal cell carcinoma (ccRCC) by constructing a computed tomography-based radiomics model and establishing a nomogram using clinicopathologic factors. The clinicopathologic parameters and genomic data were extracted from 493 ccRCC cases of the Cancer Genome Atlas (TCGA)-KIRC database. Univariate and multivariate Cox regression and Kaplan-Meier analysis were performed for prognosis analysis. Cibersortx was applied to evaluate the immune cell composition. Radiomic features were extracted from the TCGA/the Cancer Imaging Archive (TCIA) (n = 102) datasets. The support vector machine (SVM) was employed to establish the radiomics signature for predicting CTLA4 expression. Receiver operating characteristic curve (ROC), decision curve analysis (DCA), and precision-recall curve were utilized to assess the predictive performance of the radiomics signature. Correlations between radiomics score (RS) and selected features were also evaluated. An RS-based nomogram was constructed to predict prognosis. CTLA4 was significantly overexpressed in ccRCC tissues and was related to lower overall survival. A higher CTLA4 expression was independently linked to the poor prognosis (HR = 1.458, 95% CI 1.13-1.881, p = 0.004). The radiomics model for the prediction of CTLA4 expression levels (AUC = 0.769 in the training set, AUC = 0.724 in the validation set) was established using seven radiomic features. A significant elevation in infiltrating M2 macrophages was observed in the RS high group (p < 0.001). The predictive efficiencies of the RS-based nomogram measured by AUC were 0.826 at 12 months, 0.805 at 36 months, and 0.76 at 60 months. CTLA4 mRNA expression status in ccRCC could be predicted noninvasively using a radiomics model based on nephrographic phase contrast-enhanced CT images. The nomogram established by combining RS and clinicopathologic factors could predict overall survival for ccRCC patients. Our findings may help stratify prognosis of ccRCC patients and identify those who may respond best to ICI-based treatments.

He H ，Jin Z ，Dai J ，Wang H ，Sun J ，Xu D ... -  《-》

Differentiation of Clear Cell and Non-clear-cell Renal Cell Carcinoma through CT-based Radiomics Models and Nomogram.

The aim of the study was to investigate the feasibility of discriminating between clear-cell renal cell carcinoma (ccRCC) and non-clear-cell renal cell carcinoma (non-ccRCC) via radiomics models and nomogram. The retrospective study included 147 patients (ccRCC=100, non-ccRCC=47) who underwent enhanced CT before surgery. CT images of the corticomedullary phase (CMP) were collected and features from the images were extracted. The data were randomly grouped into training and validation sets according to 7:3, and then the training set was normalized to extract the normalization rule for the training set, and then the rule was applied to the validation set. First, the T-test, T'-test or Wilcoxon rank-sum test were executed in the training set data to keep the statistically different parameters, and then the optimal features were picked based on the least absolute shrinkage and selection operator (LASSO) algorithm. Five machine learning (ML) models were trained to differentiate ccRCC from noccRCC, rad+cli nomogram was constructed based on clinical factors and radscore (radiomics score), and the performance of the classifier was mainly measured by area under the curve (AUC), accuracy, sensitivity, specificity, and F1. Finally, the ROC curves and radar plots were plotted according to the five performance parameters. 1130 radiomics features were extracted, there were 736 radiomics features with statistical differences were obtained, and 4 features were finally selected after the LASSO algorithm. In the validation set of this study, three of the five ML models (logistic regression, random forest and support vector machine) had excellent performance (AUC 0.9-1.0) and two models (adaptive boosting and decision tree) had good performance (AUC 0.7-0.9), all with accuracy ≥ 0.800. The rad+cli nomogram performance was found excellent in both the training set (AUC = 0.982,0.963-1.000, accuracy=0.941) and the validation set (AUC = 0.949,0.885-1.000, accuracy=0.911). The random forest model with perfect performance (AUC = 1, accuracy=1) was found superior compared to the model performance in the training set. The rad+cli nomogram model prevailed in the comparison of the model's performance in the validation set. The ML models and nomogram can be used to identify the relatively common pathological subtypes in clinic and provide some reference for clinicians.

Cheng D ，Abudikeranmu Y ，Tuerdi B 《-》

Radiomics nomogram based on CT radiomics features and clinical factors for prediction of Ki-67 expression and prognosis in clear cell renal cell carcinoma: a two-center study.

To develop and validate a radiomics nomogram combining radiomics features and clinical factors for preoperative evaluation of Ki-67 expression status and prognostic prediction in clear cell renal cell carcinoma (ccRCC). Two medical centers of 185 ccRCC patients were included, and each of them formed a training group (n = 130) and a validation group (n = 55). The independent predictor of Ki-67 expression status was identified by univariate and multivariate regression, and radiomics features were extracted from the preoperative CT images. The maximum relevance minimum redundancy (mRMR) and the least absolute shrinkage and selection operator algorithm (LASSO) were used to identify the radiomics features that were most relevant for high Ki-67 expression. Subsequently, clinical model, radiomics signature (RS), and radiomics nomogram were established. The performance for prediction of Ki-67 expression status was validated using area under curve (AUC), calibration curve, Delong test, decision curve analysis (DCA). Prognostic prediction was assessed by survival curve and concordance index (C-index). Tumour size was the only independent predictor of Ki-67 expression status. Five radiomics features were finally identified to construct the RS (AUC: training group, 0.821; validation group, 0.799). The radiomics nomogram achieved a higher AUC (training group, 0.841; validation group, 0.814) and clinical net benefit. Besides, the radiomics nomogram provided a highest C-index (training group, 0.841; validation group, 0.820) in predicting prognosis for ccRCC patients. The radiomics nomogram can accurately predict the Ki-67 expression status and exhibit a great capacity for prognostic prediction in patients with ccRCC and may provide value for tailoring personalized treatment strategies and facilitating comprehensive clinical monitoring for ccRCC patients.

Li B ，Zhu J ，Wang Y ，Xu Y ，Gao Z ，Shi H ，Nie P ，Zhang J ，Zhuang Y ，Wang Z ，Yang G ... -  《-》