Development of a Comparative European Orthohantavirus Microneutralization Assay With Multi- Species Validation and Evaluation in a Human Diagnostic Cohort.

Orthohantaviruses (family Hantaviridae, order Bunyavirales) can cause two serious syndromes in humans: hemorrhagic fever with renal syndrome (HFRS), associated with the Old World orthohantaviruses, and hantavirus cardiopulmonary syndrome (HCPS), associated with orthohantaviruses in the Americas. In Europe, four different orthohantaviruses (DOBV, PUUV, SEOV, and TULV) are associated with human disease. As disease severity and zoonotic source differ between orthohantavirus species, conclusive determination of the infecting species by either RT-PCR or comparative virus neutralization test (VNT) is of importance. Currently, the focus reduction neutralization test (FRNT) is considered the 'Gold Standard' for orthohantavirus VNTs, however this test is laborious and time-consuming. Consequently, more high-throughput alternatives are needed. In this study, we developed a comparative orthohantavirus microneutralization test (MNT) including all four human pathogenic orthohantavirus species circulating in Europe. The assay was validated using RT-PCR-confirmed rodent (n=17) and human sera (n=17), DOBV-suspected human sera (n=3) and cohorts of orthohantavirus-negative rodent (n=3) and human sera (n=85). 16/17 RT-PCR-confirmed rodent sera and 18/20 of the RT-PCR-confirmed and DOBV-suspected human sera were serotyped successfully, while for the remaining rodent (n=1) and human sera (n=2) no neutralizing titers could be detected. All negative control sera tested negative in the MNT. The assay was subsequently evaluated using a clinical cohort of 50 orthohantavirus patients. Orthohantavirus infection was confirmed in all 50 patients, and 47/50 (94%) sera were serotyped successfully, confirming PUUV as the major cause of orthohantavirus infections in Netherlands. Notably, two previously unrecognized SEOV cases from 2013 were diagnosed using the MNT, underlining the added value of the MNT in a diagnostic setting. In conclusion, we demonstrate the successful development and clinical implementation of a comparative European orthohantavirus MNT to determine the infecting virus species in European HFRS patients. Identification of the causative species is needed for an adequate Public Health response and can support individual patient care. For many labs, the implementation of orthohantavirus neutralization tests has not been a straightforward procedure. This issue will be addressed by the rollout of the comparative MNT to multiple European laboratories to support patient diagnostics, surveillance and Public Health responses.

Hoornweg TE ，Zutt I ，de Vries A ，Maas M ，Hoogerwerf MN ，Avšič-Županc T ，Korva M ，Reimerink JHJ ，Reusken CBEM ... -  《Frontiers in Cellular and Infection Microbiology》

Use of a diagnostic Puumala virus real-time RT-PCR in an orthohantavirus endemic region in the Netherlands.

Geeraedts F ，Wevers M ，Bosma F ，Boer Md ，Brinkman JN ，Delsing C ，GeurtsvanKessel C ，Rockx B ，van der Zanden A ，Laverman GD ... -  《Microbiology Spectrum》

Puumala and Dobrava viruses cause hemorrhagic fever with renal syndrome in Bosnia-Herzegovina: evidence of highly cross-neutralizing antibody responses in early patient sera.

Lundkvist A ，Hukic M ，Hörling J ，Gilljam M ，Nichol S ，Niklasson B ... -  《JOURNAL OF MEDICAL VIROLOGY》

External quality assessment of orthohantavirus and lymphocytic choriomeningitis virus molecular detection and serology in Europe, 2021.

Erdin M ，Stanoeva KR ，Mögling R ，Korva M ，Knap N ，Resman Rus K ，Domingo C ，Reimerink JH ，de Vries A ，Alburkat H ，Utriainen M ，Gossner CM ，Sironen T ，Avšič-Županc T ，Reusken CB ，Vapalahti O ... -  《-》

Serological analysis of hemorrhagic fever with renal syndrome (HFRS) patients in Far Eastern Russia and identification of the causative hantavirus genotype.

Miyamoto H ，Kariwa H ，Araki K ，Lokugamage K ，Hayasaka D ，Cui BZ ，Lokugamage N ，Ivanov LI ，Mizutani T ，Iwasa MA ，Yoshimatsu K ，Arikawa J ，Takashima I ... -  《ARCHIVES OF VIROLOGY》