Foliar exposure of zinc oxide nanoparticles improved the growth of wheat (Triticum aestivum L.) and decreased cadmium concentration in grains under simultaneous Cd and water deficient stress.

A pot study was conducted to explore the effectiveness of zinc oxide nanoparticles (ZnO NPs) foliar exposure on growth and development of wheat, zinc (Zn) and cadmium (Cd) uptake in Cd-contaminated soil under various moisture conditions. Four different levels (0, 25, 50, 100 mg/L) of these NPs were foliar-applied at different time periods during the growth of wheat. Two soil moisture regimes (70% and 35% of water holding capacity) were maintained from 6 weeks of germination till plant harvesting. The results revealed that the growth of wheat increased with ZnO NPs treatments. The best results were found in 100 mg/L ZnO NPs under normal moisture level. The lowest Cd and highest Zn concentrations were also examined when 100 mg/L NPs were applied without water deficit stress. In grain, Cd concentrations decreased by 26%, 81% and 87% in normal moisture while in water deficit conditions, the Cd concentrations decreased by 35%, 66% and 81% compared to control treatment when ZnO NPs were used at 25, 50 and 100 mg/L. The foliar exposure of ZnO NPs boosted up the leaf chlorophyll contents and also decreased the oxidative stress and enhanced the leaf superoxide dismutase and peroxidase activities than the control. It can be suggested that foliar use of ZnO NPs might be an efficient way for increasing wheat growth and yield with maximum Zn and minimum Cd contents under drought stress while decreasing the chances of NPs movement to other environmental compartment which may be possible in soil applied NPs.

Adrees M ，Khan ZS ，Hafeez M ，Rizwan M ，Hussain K ，Asrar M ，Alyemeni MN ，Wijaya L ，Ali S ... -  《-》

Combined use of different nanoparticles effectively decreased cadmium (Cd) concentration in grains of wheat grown in a field contaminated with Cd.

Cadmium (Cd) accumulation in arable lands has become a serious matter for food security. Among various approaches, the application of nanoparticles (NPs) for remediation of contaminated water and soils is attaining more popularity worldwide. The current field experiment was executed to explore the impacts of single and combined use of ZnO NPs, Fe NPs and Si NPs on wheat growth and Cd intake by plants in a Cd-contaminated field. Wheat was sown in a field which was contaminated with Cd and was irrigated with the raw-city-effluent while NPs were applied as foliar spray alone and in all possible combinations. The data revealed that straw and grain yields were enhanced in the presence of NPs over control. Chlorophyll, carotenoids contents and antioxidants activities were enhanced while electrolyte leakage was reduced with all NPs over control. In comparison with control, Cd uptake in wheat straw was reduced by 84% and Cd uptake in grain was reduced by 99% in T8 where all three NPs were foliar-applied simultaneously. Zinc (Zn) and iron (Fe) contents were increased in those plants where ZnO and Fe NPs were exogenously applied which revealed that ZnO and Fe NPs enhanced the bio-fortification of Zn and Fe in wheat grains. Overall, foliar application of different NPs is beneficial for better wheat growth, yield, nutrients uptake and to lessen the Cd intake by plants grown in Cd-contaminated soil under real field conditions.

Hussain A ，Rizwan M ，Ali S ，Rehman MZU ，Qayyum MF ，Nawaz R ，Ahmad A ，Asrar M ，Ahmad SR ，Alsahli AA ，Alyemeni MN ... -  《-》

Zinc and iron oxide nanoparticles improved the plant growth and reduced the oxidative stress and cadmium concentration in wheat.

The effects of seed priming with zinc oxide (ZnO) and iron (Fe) nanoparticles (NPs) on the growth and cadmium (Cd) accumulation by wheat (Triticum aestivum) were investigated. Seeds of wheat were primed with different concentrations of either ZnO NPs (0, 25, 50, 75, and 100 mg L-1) or Fe NPs (0, 5, 10, 15, and 20 mg L-1) for 24 h by continuous aeration and then the seeds were sown in a soil which was contaminated with Cd due to long-term application of sewage water. Plants were grown till maturity under natural conditions with 60-70% moisture contents of total soil water holding capacity throughout the experiment. Plant height, spike length, and dry weights of shoots, roots, spikes, and grains were increased with NPs, in particular with the higher rates of NPs. The results depicted that NPs positively affected the photosynthesis of wheat as compared to the control. The NPs reduced the electrolyte leakage and superoxide dismutase and peroxidase activities in leaves of Cd-stressed wheat. The concentrations of Cd in roots, shoots, and grains were significantly decreased with NPs application. The Cd content in the grains was below the threshold level of Cd (0.2 mg kg-1) for cereals when the seeds were treated with higher NPs treatments. The application of ZnO NPs increased the Zn concentrations and Fe NPs increased the Fe concentrations in roots, shoots, and grains. Overall, the NPs play a major role in the increase in biomass, nutrients and decrease in Cd toxicity in wheat.

Rizwan M ，Ali S ，Ali B ，Adrees M ，Arshad M ，Hussain A ，Zia Ur Rehman M ，Waris AA ... -  《-》

Zinc oxide nanoparticles alter the wheat physiological response and reduce the cadmium uptake by plants.

An experiment was performed to explore the interactive impacts of zinc oxide nanoparticles (ZnO NPs) and cadmium (Cd) on growth, yield, antioxidant enzymes, Cd and zinc (Zn) concentrations in wheat (Triticum aestivum). The ZnO NPs were applied both in Cd-contaminated soil and foliar spray (in separate studies) on wheat at different intervals and plants were harvested after physiological maturity. Results depicted that ZnO NPs enhanced the growth, photosynthesis, and grain yield, whereas Cd and Zn concentrations decreased and increased respectively in wheat shoots, roots and grains. The Cd concentrations in the grains were decreased by 30-77%, and 16-78% with foliar and soil application of NPs as compared to the control, respectively. The ZnO NPs reduced the electrolyte leakage while increased SOD and POD activities in leaves of wheat. It can be concluded that ZnO NPs (levels used in the study) could effectively reduce the toxicity and concentration of Cd in wheat whereas increase the Zn concentration in wheat. Thus, ZnO NPs might be helpful in decreasing Cd and increasing Zn biofortification in cereals which might be effective to reduce the hidden hunger in humans owing the deficiency of Zn in cereals.

Hussain A ，Ali S ，Rizwan M ，Zia Ur Rehman M ，Javed MR ，Imran M ，Chatha SAS ，Nazir R ... -  《-》

Foliar spray of silicon nanoparticles improved the growth and minimized cadmium (Cd) in wheat under combined Cd and water-limited stress.

The effects of foliar supply of silicon nanoparticles (Si-NPs) on growth, physiology, and cadmium (Cd) uptake by wheat (Triticum aestivum L.) were examined in different soil moisture levels. Seeds were sown in soil containing excess Cd (7.67 mg kg-1) and Si-NPs were applied through foliar dressing with various levels (0, 25, 50, 100 mg L-1) at different time intervals during growth period. Initially, all pots were irrigated with normal moisture level (70% water-holding capacity) and two moisture levels (35%, 70% WHC) were initiated after 6 weeks of plant growth for remaining growth duration and harvesting was done after 124 days of sowing. The results demonstrated the lowest plant growth, yield, and chlorophyll concentrations while the highest oxidative stress and Cd concentrations in plant tissues in water-stressed control (35% WHC) followed by normal control (75% WHC). Si-NPs enhanced the growth, photosynthesis, leaf defense system, and Si concentrations in tissues while minimized the Cd in wheat parts particularly in grains either soil normal or water-stressed conditions. Of the foliar spray, 100 mg L-1 of Si-NPs showed the best results with respect to growth, Cd and Si uptake by plants, and soil post-harvest bioavailable Cd irrespective of soil water levels. In grain, Cd concentration was below threshold limit (0.2 mg kg-1) for cereals in 100-mg kg-1 Si-NPs treatment irrespective of soil water levels. Si-NPs foliar dressing under Cd and water-limited stress might be an effective strategy in increasing growth, yield, and decreasing Cd concentration in wheat grains under experimental conditions. Thus, foliar dressing of Si-NPs minimized the Cd risk in food crops and NPs entry to surroundings, which might be possible after harvesting of crops in soil-applied NPs.

Adrees M ，Khan ZS ，Rehman MZU ，Rizwan M ，Ali S ... -  《-》