Characterization of low-cost glycolipoprotein biosurfactant produced by Lactobacillus plantarum 60 FHE isolated from cheese samples using food wastes through response surface methodology and its potential as antimicrobial, antiviral, and anticancer activi

Considering the need of new lactic acid bacteria (LAB) for the production of novel biosurfactant (BS) molecules, the current study brings out a new insight on the exploration of cheese samples for BS producers and process optimization for industrial applications. In view of this, Lactobacillus plantarum 60FHE, Lactobacillus paracasei 75FHE, and Lactobacillus paracasei 77FHE were selected as the most operative strains. The biosurfactants (BSs) described as glycolipoproteins via Fourier-transform infrared spectroscopy (FTIR) exhibited antimicrobial activity against the food-borne pathogens. L. plantarum 60FHE BS showed an anticancer activity against colon carcinoma cells and had a week antiviral activity against Hepatitis A virus. Furthermore, glycolipoprotein production was enhanced by 1.42-fold through the development of an optimized process using central composite design (CCD). Emulsifying activities were stable after 60-min incubation from 4 to 120 °C, at pH 2-12, and after the addition of NaCl (2-14%). Characterization by nuclear magnetic resonance spectroscopy (1H NMR) revealed that BS produced from strain 60FHE was glycolipoprotein. L. plantarum produced mixed BSs determined by Liquid Chromatography/Mass Spectrometry (LC-MS). Thus, indicating that BS was applied as a microbial food prevention and biomedical. Also, L. plantarum 60FHE BS was achieved with the use of statistical optimization on inexpensive food wastes.

Sakr EAE ，Ahmed HAE ，Abo Saif FAA 《-》

Antimicrobial potential of a lipopeptide biosurfactant derived from a marine Bacillus circulans.

Das P ，Mukherjee S ，Sen R 《-》

Antimicrobial, anti-adhesive and anti-biofilm potential of biosurfactants isolated from Pediococcus acidilactici and Lactobacillus plantarum against Staphylococcus aureus CMCC26003.

Biosurfactants (BS) are amphipathic compounds produced by diverse groups of microorganisms exhibiting various biological activities. The current study aimed to assess antimicrobial, anti-adhesive and anti-biofilm activities of BS isolated from lactic acid bacteria (LAB), including Pediococcus acidilactici and Lactobacillus plantarum against Staphylococcus aureus CMCC 26003 in vitro. Cell-bound BS from both Pediococcus acidilactici and Lactobacillus plantarum were extracted, and their surface activities were evaluated by oil spreading assay. As quantified by crystal violet method, BS inhibited adhesion and biofilm formation of Staphylococcus aureus in a dose-dependent manner. The above findings were further supported by results of scanning electron microscopy. These two kinds of BS affect expressions of biofilm-related genes (cidA, icaA, dltB, agrA, sortaseA and sarA) and interfere with the release of signaling molecules (AI-2) in quorum sensing systems. Biological activities observed for BS produced by tested LAB suggest prospects for their use against Staphylococcus aureus biofilm-related infections.

Yan X ，Gu S ，Cui X ，Shi Y ，Wen S ，Chen H ，Ge J ... -  《-》

Antimicrobial and antiadhesive properties of a biosurfactant isolated from Lactobacillus paracasei ssp. paracasei A20.

Gudiña EJ ，Rocha V ，Teixeira JA ，Rodrigues LR ... -  《-》

Lactobacillus plantarum-derived biosurfactant: Ultrasound-induced production and characterization.

The aim of the present study was to investigate the effect of ultrasonic treatment (25 kHz) on biosurfactant production by Lactobacillus plantarum ATCC 8014. The impacts of the ultrasonication (with a frequency of 25 kHz and power of 7.4 W for 30 min time duration) were examined at different stages of the fermentation process to obtain the optimum stimulation instant(s). The optimum scenario was found to be one-time sonication at the 12th hour of fermentation which can be beneficial from an economic point of view (compared with multiple applications of sonication). Ultrasonic treatment at this time resulted in enhancement of the productivities of biomass (4.5 g/L) and biosurfactant (2.01 g/L) which was almost 1.3 times higher than those of the non-sonicated control samples. According to our results, it was clearly observed that glucose consumption increased after ultrasonic treatment representing the improved substrate uptake and progression of the cellular metabolism. Furthermore, the transmission electron microscopic images immediately after sonication clarified the pore formation on the cell surfaces. The results also indicated the enhancement of plasma membrane permeability of the sonicated cells. Fourier transform infrared spectroscopy and scanning electron microscopy coupled with energy dispersive x-ray spectroscopy analyses also disclosed respectively no structural differences before and after ultrasonic exposure in the produced biosurfactant and bacterial cell membrane. The biosurfactant was characterized to be a mixture of carbohydrate (28%), protein (23%) and lipid (specified by gas chromatography-mass spectrometry) known as glycolipoprotein. The sustainable critical micelle concentration and the stability of the synthesized biosurfactant can feature its potential applicability in various processes in the food and pharmaceutical industries.

Behzadnia A ，Moosavi-Nasab M ，Tiwari BK ，Setoodeh P ... -  《-》