High mobility group box 3 promotes cervical cancer proliferation by regulating Wnt/β-catenin pathway.

High mobility group box 3 (HMGB3) plays an important role in the development of various cancer. This study aims to explore whether HMGB3 regulates cervical cancer (CC) progression and elucidate the underlying mechanism. HMGB3 expression in clinical patients' tumor samples were determined by real-time quantitative polymerase chain reaction (qRT-PCR) and western blot. HMGB3 overexpression/knockdown were used to investigate its function. Cell apoptosis and cycle were detected by Annexin V/PI staining and flow cytometry. In vivo tumor model was made by subcutaneous injection of HeLa cells transfected with shRNAs targeting HMGB3 (sh-HMGB31) into the flank area of nude mice. Western blot was used to detect the levels of β-catenin, c-Myc, and matrix metalloproteinase-7 (MMP-7) in Hela and CaSki cells transfected with sh-HMGB3 or shRNAs targeting β-catenin. Both messenger RNA and protein levels of HMGB3 were upregulated in CC tissues from patients. High expression level of HMGB3 had positive correlation with serosal invasion, lymph metastasis, and tumor sizes in CC patient. Functional experiments showed that HMGB3 could promote CC cell proliferation both in vitro and in vivo. The expression levels of c-Myc and MMP-7 were increased, resulting in regulating cell apoptosis, cell cycle, and activating Wnt/β-catenin pathway. Our data indicated that HMGB3 may serve as an oncoprotein. It could be used as a potential prognostic marker and represent a promising therapeutic strategy for CC treatment.

Zhuang S ，Yu X ，Lu M ，Li Y ，Ding N ，Ding Y ... -  《-》

Long Noncoding RNA SOX2-OT Knockdown Inhibits Proliferation and Metastasis of Prostate Cancer Cells Through Modulating the miR-452-5p/HMGB3 Axis and Inactivating Wnt/β-Catenin Pathway.

Song X ，Wang H ，Wu J ，Sun Y ... -  《-》

HMGB3 promotes growth and migration in colorectal cancer by regulating WNT/β-catenin pathway.

Zhang Z ，Chang Y ，Zhang J ，Lu Y ，Zheng L ，Hu Y ，Zhang F ，Li X ，Zhang W ，Li X ... -  《PLoS One》

High mobility group-box 3 overexpression is associated with poor prognosis of resected gastric adenocarcinoma.

Tang HR ，Luo XQ ，Xu G ，Wang Y ，Feng ZJ ，Xu H ，Shi YW ，Zhang Q ，Wu LG ，Xue CQ ，Wang CW ，Wu CY ... -  《-》

High-mobility group box 3 (HMGB3) silencing inhibits non-small cell lung cancer development through regulating Wnt/β-catenin pathway.

Li Y ，Ma Y ，Zhang T ，Feng C ，Liu Y ... -  《-》