The transcription factor USF1 promotes glioma cell invasion and migration by activating lncRNA HAS2-AS1.

The role of lncRNAs in tumor has been widely concerned. The present study took HAS2-AS1 (the antisense RNA 1 of HAS2) as a starting point to explore its expression in glioma and its role in the process of migration and invasion, providing a strong theoretical basis for mining potential therapeutic targets of glioma. Clinical data of glioma were obtained from The Cancer Genome Atlas (TCGA) database and differentially expressed lncRNAs were analyzed by edgeR. The hTFtarget database was used to predict the upstream transcription factors of HAS2-AS1 and the JASPAR website was used to predict the binding sites of human upstream transcription factor 1 (USF1) and HAS2-AS1. qRT-PCR was used to detect the expressions of HAS2-AS1 and USF1 in glioma tissues and cell lines. The effects of silencing HAS2-AS1 on the migration and invasion of cancer cells were verified by wound healing and Transwell invasion assays. The chromatin immunoprecipitation (ChIP) and dual luciferase reporter assays were applied to demonstrate the binding of USF1 and HAS2-AS1 promoter region. Western blot was used to detect the expressions of epithelial-mesenchymal transition (EMT)-related proteins. HAS2-AS1 was highly expressed in glioma tissues and cells, and was significantly associated with poor prognosis. Silencing HAS2-AS1 expression inhibited glioma cell migration, invasion and EMT. USF1 was highly expressed in glioma and positively correlated with HAS2-AS1. The transcription of HAS2-AS1 was activated by USF1 via binding to HAS2-AS1 promoter region, consequently potentiating the invasion and migration abilities of glioma cells. These results suggested that the transcription factor USF1 induced up-regulation of lncRNA HAS2-AS1 and promoted glioma cell invasion and migration.

Wang J ，Gu J ，You A ，Li J ，Zhang Y ，Rao G ，Ge X ，Zhang K ，Li J ，Liu X ，Wang Q ，Lin T ，Cheng L ，Zhu M ，Wu X ，Wang D ... -  《-》

HAS2-AS1 Acts as a Molecular Sponge for miR-137 and Promotes the Invasion and Migration of Glioma Cells by Targeting EZH2.

Wang J ，Zhang Y ，You A ，Li J ，Gu J ，Rao G ，Ge X ，Zhang K ，Fu H ，Liu X ，Li J ，Wang Q ，Wu X ，Cheng L ，Zhu M ，Wang D ... -  《-》

Long Noncoding RNA ASB16-AS1 Promotes Proliferation, Migration, and Invasion in Glioma Cells.

Zhang D ，Zhou H ，Liu J ，Mao J ... -  《-》

Long non-coding RNA PAXIP1-AS1 facilitates cell invasion and angiogenesis of glioma by recruiting transcription factor ETS1 to upregulate KIF14 expression.

Xu H ，Zhao G ，Zhang Y ，Jiang H ，Wang W ，Zhao D ，Yu H ，Qi L ... -  《JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH》

A Long Noncoding RNA ZEB1-AS1 Promotes Tumorigenesis and Predicts Poor Prognosis in Glioma.

Lv QL ，Hu L ，Chen SH ，Sun B ，Fu ML ，Qin CZ ，Qu Q ，Wang GH ，He CJ ，Zhou HH ... -  《INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES》