Colorectal basidiobolomycosis in a dog.

A 7-year-old castrated male French Bulldog was examined for chronic large intestinal enteropathy. A colonic mass and thickened rectal mucosa were identified, and histopathologic examination of endoscopic biopsy specimens disclosed eosinophilic proctitis with large (5-20 μm), irregularly shaped, pauciseptate hyphae that were Gomori methenamine silver and periodic acid-Schiff positive. Amplification and sequencing of ribosomal DNA extracted from paraffin-embedded tissues yielded a sequence with 97% identity to GenBank sequences for Basidiobolus ranarum. After itraconazole, terbinafine, and prednisone administration, clinical signs resolved rapidly, and sonographic lesions were largely absent after 6 weeks. Treatment was discontinued by the owner 15 weeks after diagnosis. Three weeks later, the dog collapsed acutely and was euthanized. Necropsy identified metastatic islet cell carcinoma and grossly unremarkable colorectal tissues. However, histopathology of the rectum disclosed multifocal submucosal granulomas with intralesional hyphae morphologically similar to those previously observed. This report is the first to describe medical treatment of gastrointestinal basidiobolomycosis in a dog.

Marclay M ，Langohr IM ，Gaschen FP ，Rodrigues-Hoffmann A ，Carossino M ，Stewart MA ，Myers AN ，Grooters AM ... -  《-》

Gastrointestinal pythiosis with concurrent presumptive gastrointestinal basidiobolomycosis in a Boxer dog.

A 2-year-old female spayed Boxer dog was presented for a 1-month history of progressive hemorrhagic diarrhea with tenesmus and weight loss despite trial courses of antibiotics and diet change. Abdominal ultrasound revealed severe, focal thickening, and loss of normal architecture of the colonic wall with abdominal lymphadenomegaly. Dry-mount fecal cytology, performed on several consecutive days, consistently revealed numerous, round, 16-20 μm structures with basophilic, granular content, and a thin cell wall. Transmission electron microscopy identified these structures as fungi. Culture, polymerase chain reaction (PCR), and sequencing of the internal transcribed spacer, D1/D2 regions, and DNA-directed RNA polymerase II core subunit (RPB2) confirmed the presence of Basidiobolus microsporus in the feces. Biopsies collected via ileocolonoscopy revealed marked, multifocal, chronic, neutrophilic, and eosinophilic ileitis and colitis with ulceration, granulation tissue, and intralesional hyphae (identified with Gomori methenamine silver stain). A Pythium enzyme-linked immunosorbent assay and Pythium-specific PCR performed on the formalin-fixed paraffin-embedded biopsy specimens were positive while Basidiobolus-specific PCR was negative, thus confirming a diagnosis of pythiosis. This report describes a fatal case of colonic and intestinal pythiosis with the presence of fecal Basidiobolus sp. spores, suggestive of concurrent gastrointestinal basidiobolomycosis.

Parambeth JC ，Lawhon SD ，Mansell J ，Wu J ，Clark SD ，Sutton D ，Gibas C ，Wiederhold NP ，Myers AN ，Johnson MC ，Levine GJ ，Schlemmer S ，Ruoff C ，Isaiah A ，Creevy KE ... -  《-》

Gastrointestinal basidiobolomycosis in a dog.

Okada K ，Amano S ，Kawamura Y ，Kagawa Y ... -  《-》

Infection with Basidiobolus ranarum in two dogs.

Greene CE ，Brockus CW ，Currin MP ，Jones CJ ... -  《JAVMA-JOURNAL OF THE AMERICAN VETERINARY MEDICAL ASSOCIATION》

Successful treatment of extensive basidiobolomycosis with oral itraconazole in a child.

Mathew R ，Kumaravel S ，Kuruvilla S ，Varghese RG ，Shashikala ，Srinivasan S ，Mani MZ ... -  《INTERNATIONAL JOURNAL OF DERMATOLOGY》