Cyclooxigenase-2 and osteopontin in gastric pre-neoplastic lesions in relation to H-pylori infection and grade of inflammation.

The association between Helicobacter-pylori-induced inflammation and gastric adenocarcinoma is well documented and it has been suggested that the pro-mitotic and apoptotic effect of Cyclooxygenase-2 and Osteopontin on the epithelial cells of the gastric mucosa may have a role in carcinogenesis of the gastric mucosa. The aim of this study was to determine the expression of Cyclooxygenase-2 and Osteopontin in normal gastric mucosa, mucosa with gastritis and gastric mucosa with intestinal metaplasia, in relation to Helicobacter-pylori infection and grade of inflammation. Immunohistochemistry was performed on 108 gastric biopsies in order to detect Cyclooxygenase-2 and Osteopontin expression. The intensity and percentage of staining were evaluated using the H-Score, and its association with grade of inflammation, Helicobacter pylori infection and intestinal metaplasia was determined. Expression of Cyclooxygenase-2 and Osteopontin was higher in gastric biopsies (values shown respectively) with Helicobacter-pylori infection (179.9/142.3), intestinal metaplasia (208.8/179.3) or higher grades of inflammation (190/135.7) in comparison to normal gastric mucosa (100.7/80) or mild grade of inflammation (128.4/128.4), (p<0.05).There is an overexpression of Cyclooxygenase-2 and Osteopontin in gastric mucosa with H. pylori infection, intestinal metaplasia and high grades of inflammation, suggesting a constant up-regulation of protein expression in response to the inflammatory process generated by a Helicobacter-pylori infection, leading to the development of intestinal metaplasia.

Benedetti I ，Hoyos J ，Barrios L 《-》

Correlations among Helicobacter pylori infection and the expression of cyclooxygenase-2 and vascular endothelial growth factor in gastric mucosa with intestinal metaplasia or dysplasia.

Liu D ，He Q ，Liu C 《-》

[Effect of Helicobacter pylori infection and eradication on proliferative kinetics of the gastric mucosa].

Unger Z ，Prónai L ，Szaleczky E ，Zágoni T ，Molnár B ，Tulassay Z ... -  《ORVOSI HETILAP》

High expression of Helicobacter pylori VapD in both the intracellular environment and biopsies from gastric patients with severity.

Morales-Espinosa R ，Delgado G ，Serrano LR ，Castillo E ，Santiago CA ，Hernández-Castro R ，Gonzalez-Pedraza A ，Mendez JL ，Mundo-Gallardo LF ，Manzo-Merino J ，Ayala S ，Cravioto A ... -  《PLoS One》

[Effect of Helicobacter pylori on gastric epithelial cell kinetics and expression of apoptosis-related proteins in gastric carcinogenesis].

Lee KM ，Lee DS ，Yang JM ，Ahn BM ，Lee EH ，Yoo JY ，Kim YJ ，Chung IS ，Sun HS ，Park DH ... -  《-》