MiR-328-3p inhibits cell proliferation and metastasis in colorectal cancer by targeting Girdin and inhibiting the PI3K/Akt signaling pathway.

MiR-328-3p has been reported to be downregulated and serve as a tumor suppressor in several cancers. Previous studies only have reported the downregulation of miR-328-3p in CRC. However, the roles of miR-328-3p in CRC growth and metastasis were unknown. In this study, we demonstrated that miR-328-3p overexpression inhibited cell proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT). The PI3K/Akt signaling pathway was also inactivated by miR-328-3p overexpression. MiR-328-3p knockdown showed the opposite effects. In addition, we confirmed that miR-328-3p directly bound to 3'UTR of Girdin and negatively regulated its expression. Girdin knockdown or treatment with PI3K inhibitor LY294002 blocked the effects of miR-328-3p inhibitor on cell proliferation, metastasis, and the PI3K/Akt signaling pathway. Moreover, pre-miR-328 decreased numbers of liver metastatic nodules, and reduced the levels of p-Akt, p-Girdin, and Girdin in metastatic tissues in liver. In conclusion, miR-328-3p may inhibit proliferation and metastasis of CRC cells by targeting Girdin and inactivating the PI3K/Akt signaling pathway. MiR-328-3p may be a novel target in cancer therapy.

Pan S ，Ren F ，Li L ，Liu D ，Li Y ，Wang A ，Li W ，Dong Y ，Guo W ... -  《-》

Disruption of the c-Myc/miR-200b-3p/PRDX2 regulatory loop enhances tumor metastasis and chemotherapeutic resistance in colorectal cancer.

Lv Z ，Wei J ，You W ，Wang R ，Shang J ，Xiong Y ，Yang H ，Yang X ，Fu Z ... -  《Journal of Translational Medicine》

Long noncoding RNA SNHG14 accelerates cell proliferation, migration, invasion and suppresses apoptosis in colorectal cancer cells by targeting miR-944/KRAS axis through PI3K/AKT pathway.

Pei Q ，Liu GS ，Li HP ，Zhang Y ，Xu XC ，Gao H ，Zhang W ，Li T ... -  《-》

miR-125a-3p/FUT5-FUT6 axis mediates colorectal cancer cell proliferation, migration, invasion and pathological angiogenesis via PI3K-Akt pathway.

Liang L ，Gao C ，Li Y ，Sun M ，Xu J ，Li H ，Jia L ，Zhao Y ... -  《Cell Death & Disease》

Silencing of Girdin suppresses the malignant behavior of colorectal carcinoma cells.

Lu J ，Zhang L ，Zhou H ，Du Z ，Zhang G ... -  《-》