Phaeodactylum tricornutum: A Diatom Cell Factory.

A switch from a petroleum-based to a biobased economy requires the capacity to produce both high-value low-volume and low-value high-volume products. Recent evidence supports the development of microalgae-based microbial cell factories with the objective of establishing environmentally sustainable manufacturing solutions. Diatoms display rich diversity and potential in this regard. We focus on Phaeodactylum tricornutum, a pennate diatom that is commonly found in marine ecosystems, and discuss recent trends in developing the diatom chassis for the production of a suite of natural and genetically engineered products. Both upstream and downstream developments are reviewed for the commercial development of P. tricornutum as a cell factory for a spectrum of marketable products.

Butler T ，Kapoore RV ，Vaidyanathan S 《-》

Delta 5 fatty acid desaturase upregulates the synthesis of polyunsaturated fatty acids in the marine diatom Phaeodactylum tricornutum.

Peng KT ，Zheng CN ，Xue J ，Chen XY ，Yang WD ，Liu JS ，Bai W ，Li HY ... -  《-》

Phaeodactylum tricornutum: An established model species for diatom molecular research and an emerging chassis for algal synthetic biology.

Diatoms are prominent and highly diverse microalgae in aquatic environments. Compared with other diatom species, Phaeodactylum tricornutum is an "atypical diatom" displaying three different morphotypes and lacking the usual silica shell. Despite being of limited ecological relevance, its ease of growth in the laboratory and well-known physiology, alongside the steady increase in genome-enabled information coupled with effective tools for manipulating gene expression, have meant it has gained increased recognition as a powerful experimental model for molecular research on diatoms. We here present a brief overview of how over the last 25 years P. tricornutum has contributed to the unveiling of fundamental aspects of diatom biology, while also emerging as a new tool for algal process engineering and synthetic biology.

Russo MT ，Rogato A ，Jaubert M ，Karas BJ ，Falciatore A ... -  《-》

Microalgae as bioreactors for bioplastic production.

Hempel F ，Bozarth AS ，Lindenkamp N ，Klingl A ，Zauner S ，Linne U ，Steinbüchel A ，Maier UG ... -  《Microbial Cell Factories》

Extrachromosomal Genetic Engineering of the Marine Diatom Phaeodactylum tricornutum Enables the Heterologous Production of Monoterpenoids.

Fabris M ，George J ，Kuzhiumparambil U ，Lawson CA ，Jaramillo-Madrid AC ，Abbriano RM ，Vickers CE ，Ralph P ... -  《ACS Synthetic Biology》