Fecal carriage of extended-spectrum β-lactamase-producing Enterobacteriaceae in hospital and community settings in Chad.

Fecal carriage of extended-spectrum β-lactamase-producing Enterobacteriaceae (ESBL-PE) remains poorly documented in Africa. The objective of this study was to determine the prevalence of ESBL-PE fecal carriage in Chad. In total, 200 fresh stool samples were collected from 100 healthy community volunteers and 100 hospitalized patients from January to March 2017. After screening using ESBL-selective agar plates and species identification by MALDI-TOF mass spectrometry, antibiotic susceptibility was tested using the disk diffusion method, and ESBL production confirmed with the double-disc synergy test. The different ESBL genes in potential ESBL-producing isolates were detected by PCR and double stranded DNA sequencing. Escherichia coli phylogenetic groups were determined using a PCR-based method. ESBL-PE fecal carriage prevalence was 44.5% (51% among hospitalized patients vs 38% among healthy volunteers; p < 0.05). ESBL-producing isolates were mostly Escherichia coli (64/89) and Klebsiella pneumoniae (16/89). PCR and sequencing showed that 98.8% (87/89) of ESBL-PE harbored blaCTX-M genes: blaCTX-M-15 in 94.25% (82/87) and blaCTX-M-14 in 5.75% (5/87). Phylogroup determination by quadruplex PCR indicated that ESBL-producing E. coli isolates belonged to group A (n = 17; 27%), C (n = 17; 27%), B2 (n = 9; 14%), B1 (n = 8; 13%), D (n = 8; 13%), E (n = 1; 1.6%), and F (n = 1; 1.6%). The ST131 clone was identified in 100% (9/9) of E. coli B2 strains. The high fecal carriage rate of ESBL-PE associated with CTX-M-15 in hospital and community settings of Chad highlights the risk for resistance transmission between non-pathogenic and pathogenic bacteria.

Ouchar Mahamat O ，Tidjani A ，Lounnas M ，Hide M ，Benavides J ，Somasse C ，Ouedraogo AS ，Sanou S ，Carrière C ，Bañuls AL ，Jean-Pierre H ，Dumont Y ，Godreuil S ... -  《Antimicrobial Resistance and Infection Control》

High prevalence and characterization of extended-spectrum ß-lactamase producing Enterobacteriaceae in Chadian hospitals.

Ouchar Mahamat O ，Lounnas M ，Hide M ，Dumont Y ，Tidjani A ，Kamougam K ，Abderrahmane M ，Benavides J ，Solassol J ，Bañuls AL ，Jean-Pierre H ，Carrière C ，Godreuil S ... -  《BMC INFECTIOUS DISEASES》

Fecal Carriage of Enterobacteriaceae Producing Extended-Spectrum Beta-Lactamases in Hospitalized Patients and Healthy Community Volunteers in Burkina Faso.

Ouédraogo AS ，Sanou S ，Kissou A ，Poda A ，Aberkane S ，Bouzinbi N ，Nacro B ，Ouédraogo R ，Van De Perre P ，Carriere C ，Decré D ，Jean-Pierre H ，Godreuil S ... -  《-》

High prevalence of extended-spectrum ß-lactamase producing enterobacteriaceae among clinical isolates in Burkina Faso.

Ouedraogo AS ，Sanou M ，Kissou A ，Sanou S ，Solaré H ，Kaboré F ，Poda A ，Aberkane S ，Bouzinbi N ，Sano I ，Nacro B ，Sangaré L ，Carrière C ，Decré D ，Ouégraogo R ，Jean-Pierre H ，Godreuil S ... -  《BMC INFECTIOUS DISEASES》

Fecal carriage of extended-spectrum beta-lactamase and ampc beta-lactamase-producing enterobacteriaceae in a turkish community.

Hazirolan G ，Mumcuoglu I ，Altan G ，Özmen BB ，Aksu N ，Karahan ZC ... -  《Nigerian Journal of Clinical Practice》