Silencing circular RNA hsa_circ_0004491 promotes metastasis of oral squamous cell carcinoma.

Oral squamous cell carcinoma (OSCC) is an oral cavity malignancy which is the eighth most common cancer. However, the molecular mechanisms underlying the pathogenesis of OSCC remain largely unknown, and effective methods for the prognosis and diagnosis of this disease are lacking. Circular RNAs (circRNAs) are widely expressed among mammals. A growing number of studies have shown that circRNA expression is altered in cancers. In this study, we investigated the role of hsa_circ_0004491 in OSCC cell migration and invasion and examined the clinical characteristics associated with its expression. Hsa_circ_0004491 expression was examined in 40 paired OSCC and normal tissue samples using quantitative real-time PCR (qRT-PCR). Wound healing, transwell and western blotting assays were conducted in OSCC cells up- or down-regulation of hsa_circ_0004491 to evaluate changes in cell migration, invasion and protein expression. KEYFINDINGS: qRT-PCR analysis revealed that hsa_circ_0004491 was significantly downregulated in OSCC tissues compared with paired normal tissues (P < 0.001), and this low expression was associated with lymph node metastasis (P = 0.0398; area under ROC curve = 0.7510). Hsa_circ_000449 silencing or overexpression significantly affected the invasion and migration abilities of OSCC cells. Western blotting analysis showed that EMT-related proteins expression was significantly changed after hsa_circ_000449 silencing or overexpression. The expression level of hsa_circ_0004491 affects the migration and invasion of OSCC cells. Hsa_circ_0004491 may therefore play a role in the progression of oral squamous cell carcinoma.

Li X ，Zhang H ，Wang Y ，Sun S ，Shen Y ，Yang H ... -  《-》

Silencing circular RNA hsa_circ_009755 promotes growth and metastasis of oral squamous cell carcinoma.

Oral squamous cell carcinoma (OSCC) is a common and rampant malignancy of the head and neck. However, its pathogenesis remains unclear. In this study, we have investigated the effects of circular RNA hsa_circ_009755 on proliferation, migration, invasion, and apoptosis in OSCC cells in vitro. Eight pairs of OSCC and normal adjacent tissues were selected to detect the differential expression of circRNAs by high-throughput sequencing. circRNA hsa_circ_009755 expression was determined by quantitative real-time polymerase chain reaction in OSCC tissues and cell lines. CCK-8, wound healing, Transwell, and flow cytometry assays were used to determine OSCC cell proliferation, migration, invasion, and apoptosis, respectively. The expression of hsa_circ_009755 was low in OSCC tissues and three OSCC cell lines. Silencing hsa_circ_009755 significantly enhanced the proliferation, migration, and invasion and suppressed the apoptosis of OSCC cells. Therefore, hsa_circ_009755 may be important in the tumorigenesis of OSCC.

Zhang B ，Wang Z ，Shen Y ，Yang H ... -  《-》

hsa_circ_0072387 Suppresses Proliferation, Metastasis, and Glycolysis of Oral Squamous Cell Carcinoma Cells by Downregulating miR-503-5p.

Han L ，Cheng J ，Li A 《-》

Hsa_circ_0086414 Might Be a Diagnostic Biomarker of Oral Squamous Cell Carcinoma.

Li L ，Zhang ZT 《MEDICAL SCIENCE MONITOR》

Downregulated hsa_circ_0036988 promotes proliferation and metastasis in oral squamous cell carcinoma.

Zhang H ，Zhang B ，Chen Y ，Zhang Y ，Qian M ，Yuan L ，Shen Y ，Yang H ... -  《-》