Long noncoding RNA PANDA promotes esophageal squamous carcinoma cell progress by dissociating from NF-YA but interact with SAFA.

Esophageal squamous cell carcinoma (ESCC) is one of the major global health problems, especially in Asia. Long non-coding RNAs (lncRNAs) have been increasingly identified and characterized in almost every aspect of biology, especially in cancer biology. This research desires to explore the regulatory mechanism of lncRNA PANDA (PANDA) on ESCC process. Quantitative real-time PCR (qRT-PCR) was carried out to detect the PANDA expression, which was up-regulated in matched cancerous tissues and adjacent noncancerous tissues from 134 patients and 9 ESCC cell lines. Higher expression of PANDA in ESCC tissues was associated with TNM stage, advanced clinical stage, and shorter overall survival of ESCC patients by MTT, EDU, colony formation assay and flow cytometry in KYSE180 and KYSE450 cells. Exogenous down-regulation of PANDA expression significantly suppressed ESCC cells proliferation and colony formation by arresting G1-S checkpoint transition in vitro, and retarded the development of tumors in vivo. Meanwhile, qRT-PCR and western blot assays showed that depletion of PANDA reduced E2F1, cyclinD1, cyclinD2, cyclinE1 and Bcl-2 expression. RIP showed the interaction between PANDA and NF-YA or SAFA. Our findings suggested that, PANDA drifted away from NF-YA to promote the expression of NF-YA-E2F1 co-regulated proliferation-promoting genes, and to limit the cell apoptosis. In addition, PANDA binds SAFA to switch on the tumor proliferation program through CyclinD1/2-Cyclin E1 and Bcl-2 pathways. PANDA could serve as a potential prognostic biomarker and therapeutic target for ESCC.

Shi W ，Wang Q ，Bian Y ，Fan Y ，Zhou Y ，Feng T ，Li Z ，Cao X ... -  《-》

Long non-coding RNA DUXAP8 regulates proliferation and invasion of esophageal squamous cell cancer.

Xu LJ ，Yu XJ ，Wei B ，Hui HX ，Sun Y ，Dai J ，Chen XF ... -  《-》

Up-regulation of lncRNA CASC9 promotes esophageal squamous cell carcinoma growth by negatively regulating PDCD4 expression through EZH2.

Wu Y ，Hu L ，Liang Y ，Li J ，Wang K ，Chen X ，Meng H ，Guan X ，Yang K ，Bai Y ... -  《Molecular Cancer》

LncRNA-TUSC7/miR-224 affected chemotherapy resistance of esophageal squamous cell carcinoma by competitively regulating DESC1.

Chang ZW ，Jia YX ，Zhang WJ ，Song LJ ，Gao M ，Li MJ ，Zhao RH ，Li J ，Zhong YL ，Sun QZ ，Qin YR ... -  《JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH》

Long non-coding RNA LINC00675 inhibits tumorigenesis and EMT via repressing Wnt/β-catenin signaling in esophageal squamous cell carcinoma.

Zhong YB ，Shan AJ ，Lv W ，Wang J ，Xu JZ ... -  《-》