Quinalizarin induces cycle arrest and apoptosis via reactive oxygen species-mediated signaling pathways in human melanoma A375 cells.

Quinalizarin, a bioactive and highly selective compound, is known to promote apoptosis in colon and lung cancer cells. However, studies evaluating quinalizarin-induced apoptosis in melanoma cells have not been conducted. In the present study, we investigated the underlying mechanisms of antimelanoma activity of quinalizarin in human melanoma A375 cells. The MTT assay and Trypan blue staining were used to evaluate the cell viability. The flow cytometry was used to detect cell cycle, apoptosis and reactive oxygen species (ROS). Western blot was used to detect the expression of cell cycle and apoptosis-related proteins, MAPK, and STAT3. The results revealed a significant dose and time dependent effect of quinalizarin on inhibiting proliferation in three kinds of human melanoma cells, and had no significant toxic effects on normal cells. Moreover, quinalizarin triggered G2/M phase cell arrest by modulating the protein expression levels of CDK 1/2, cyclin A, cyclin B, p21 and p27, and induced apoptosis by down-regulating the antiapoptotic protein Bcl-2 and upregulating the proapoptotic protein BAD, leading to the activation of caspase-3 and PARP in the caspase cascade in A375 cells. Quinalizarin treatment led to apoptosis of A375 cells via activation of MAPK and inhibition of STAT3 signaling pathways. In addition, quinalizarin increased the level of ROS, but ROS scavenger NAC inhibited quinalizarin-induced apoptosis by regulating MAPK and STAT3 signaling pathways. In summary, quinalizarin induces cell cycle arrest and apoptosis via ROS-mediated MAPK and STAT3 signaling pathways in human melanoma A375 cells, and quinalizarin may be used as a novel and effective antimelanoma therapeutic.

Luo YH ，Li JQ ，Zhang Y ，Wang JR ，Xu WT ，Zhang Y ，Feng YC ，Li SZ ，Jin CH ... -  《-》

Quinalizarin Induces Apoptosis through Reactive Oxygen Species (ROS)-Mediated Mitogen-Activated Protein Kinase (MAPK) and Signal Transducer and Activator of Transcription 3 (STAT3) Signaling Pathways in Colorectal Cancer Cells.

Meng LQ ，Wang Y ，Luo YH ，Piao XJ ，Liu C ，Wang Y ，Zhang Y ，Wang JR ，Wang H ，Xu WT ，Liu Y ，Wu YQ ，Sun HN ，Han YH ，Jin MH ，Shen GN ，Fang NZ ，Jin CH ... -  《MEDICAL SCIENCE MONITOR》

Benzyl isothiocyanate (BITC) induces G2/M phase arrest and apoptosis in human melanoma A375.S2 cells through reactive oxygen species (ROS) and both mitochondria-dependent and death receptor-mediated multiple signaling pathways.

Huang SH ，Wu LW ，Huang AC ，Yu CC ，Lien JC ，Huang YP ，Yang JS ，Yang JH ，Hsiao YP ，Wood WG ，Yu CS ，Chung JG ... -  《-》

Quinalizarin exerts an anti-tumour effect on lung cancer A549 cells by modulating the Akt, MAPK, STAT3 and p53 signalling pathways.

Meng LQ ，Liu C ，Luo YH ，Piao XJ ，Wang Y ，Zhang Y ，Wang JR ，Wang H ，Xu WT ，Liu Y ，Wu YQ ，Sun HN ，Han YH ，Jin MH ，Shen GN ，Zang YQ ，Li J ，Fang NZ ，Cui YD ，Jin CH ... -  《-》

Molecular mechanisms of quinalizarin induces apoptosis and G0/G1 cell cycle of human esophageal cancer HCE-4 cells depends on MAPK, STAT3, and NF-κB signaling pathways.

Zang YQ ，Zhai YQ ，Feng YY ，Ju XY ，Zuo F ... -  《-》