Recent Advances in Biosensors for Detecting Cancer-Derived Exosomes.

Early detection and effective treatments are two of the greatest challenges in the fight against cancer. Cancer-derived exosomes are attractive biomarkers for the early diagnosis and therapeutic response evaluation of cancer. Here, we review recent advances in biosensors for the detection of cancer-derived exosomes. We discuss the potential exosomal biomarkers of various cancers, which can be applied as indicative targets in the design of biosensors. We further describe the fabrication of exosome detection biosensors with respect to biological recognition strategies and signal transduction techniques, which involve integrated scientific and technological aspects of analytical chemistry and nanotechnology. Furthermore, future research directions and challenges in using cancer-derived exosomes for point-of-care (POC) testing are presented.

Cheng N ，Du D ，Wang X ，Liu D ，Xu W ，Luo Y ，Lin Y ... -  《-》

Recent Advances in Exosomal Protein Detection Via Liquid Biopsy Biosensors for Cancer Screening, Diagnosis, and Prognosis.

Liu C ，Yang Y ，Wu Y 《AAPS Journal》

Recent advances of aptasensors for exosomes detection.

Exosomes are nanoscale phospholipid bilayer membrane-enclosed vesicles released from cells with diameters of 30-150 nm. Their contents reflect significant information regarding the cancer microenvironment from their parent cells, which attracts increasing attention as potential biomarkers for noninvasive early diagnosis. Among their detection methods, aptasensor has been becoming an attractive star with its properties of affordability, easy to use, fast response, high sensitivity, remarkable specificity, and multiplexing capability. This review mainly summarizes the recent advances of single-stranded DNA (ssDNA) aptamer-based sensors for cancer and tumor-derived exosomes detection. Firstly, we present a brief overview of aptamers and exosomes. Then, we introduce the exosomal proteins used as potential biomarkers of various cancers, and their specific ssDNA aptamers used in aptasensors. We emphasize eight major types of aptasensors: fluorescent, electrochemical, colorimetric, luminescence, lateral flow strips, surface-enhanced Raman scattering, surface plasmon resonance, and giant magnetoresistance sensors, based on fabrication methods, bio-recognition mechanism, as well as detection evaluation. The future directions and challenges are finally proposed for aptamers and their more applications in exosomes research.

Zhu C ，Li L ，Wang Z ，Irfan M ，Qu F ... -  《-》

Recent advances in nanotechnology-enabled biosensors for detection of exosomes as new cancer liquid biopsy.

Hsu CC ，Wu Y 《-》

Recent advances in nanomaterial-based biosensors for the detection of exosomes.

Zhang L ，Gu C ，Wen J ，Liu G ，Liu H ，Li L ... -  《-》