The effector SIX8 is required for virulence of Fusarium oxysporum f.sp. cubense tropical race 4 to Cavendish banana.

Plant pathogens employ effectors as molecular weapons to manipulate host immunity and facilitate colonization. Fusarium oxysporum f. sp. cubense is the agent of wilt disease in banana plantlets and four races of the pathogen have been identified based on the cultivar specificity. A total of 9 SIX genes have been detected in the genome of Foc TR4 and 6 genes detected in Foc1. Among these SIX genes, SIX2 and SIX8 are only detected in Foc TR4, not identified in Foc1. Expression profiles analysis revealed that SIX genes of Foc TR4 are highly induced after inoculation to Cavendish banana plantlets. Virulence analysis of the SIX2 and SIX8 knock-out mutants showed that SIX8 is required for the virulence of Foc TR4 while SIX2 has no obvious functions. Over expression of SIX8-FLAG proteins in the SIX8 knock-out mutant partly restored the virulence. Western blot analysis suggested that SIX8 could be secreted into the extracellular space and a signal peptide resided the N-terminal polypeptide sequence. This study provides some clues for further research on mechanism of SIX8 in regulating virulence of Foc TR4.

An B ，Hou X ，Guo Y ，Zhao S ，Luo H ，He C ，Wang Q ... -  《Fungal Biology》

Dual species transcript profiling during the interaction between banana (Musa acuminata) and the fungal pathogen Fusarium oxysporum f. sp. cubense.

Li W ，Wang X ，Li C ，Sun J ，Li S ，Peng M ... -  《BMC GENOMICS》

SGE1 is involved in conidiation and pathogenicity of Fusarium oxysporum f.sp. cubense.

Hou X ，An B ，Wang Q ，Guo Y ，Luo H ，He C ... -  《-》

Draft Genome of Fusarium oxysporum f. sp. cubense Strain Tropical Race-4 Infecting Cavendish (AAA) Group of Banana in India.

Thangavelu R ，Edwin Raj E ，Pushpakanth P ，Loganathan M ，Uma S ... -  《PLANT DISEASE》

A Cerato-Platanin Family Protein FocCP1 Is Essential for the Penetration and Virulence of Fusarium oxysporum f. sp. cubense Tropical Race 4.

Liu S ，Wu B ，Yang J ，Bi F ，Dong T ，Yang Q ，Hu C ，Xiang D ，Chen H ，Huang H ，Shao C ，Chen Y ，Yi G ，Li C ，Guo X ... -  《INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES》