Occurrence of substituted diphenylamine antioxidants and benzotriazole UV stabilizers in Arctic seabirds and seals.

Substituted diphenylamine antioxidants (SDPAs) and benzotriazole UV stabilizers (BZT-UVs) are contaminants of emerging environmental concern. However, little is known about the occurrence of these contaminants in the Arctic. In this study, we investigated the levels of 11 SDPAs and 6 BZT-UVs in livers and eggs of two seabird species, the black-legged kittiwake (Rissa tridactyla) and northern fulmar (Fulmarus glacialis), as well as the liver of ringed seals (Pusa hispida) from Canadian high- and sub-Arctic sites. The concentrations of ΣSDPAs in seabird livers (median 336 pg g-1, wet weight (ww)) were significantly higher than the eggs (median 24 pg g-1, ww) and the seal livers (median 38 pg g-1, ww), suggesting liver was a primary tissue of SDPA accumulation in seabirds and that seabirds were at greater risk of exposure to SDPAs than marine mammals in the Arctic. The predominant SDPA was monostyryl octyl-diphenylamine and this compound was detected in every seabird and seal sample, indicating the widespread distribution of this contaminant in Arctic food webs. Unlike SDPAs, the detection rate and concentrations of BZT-UVs in seals were higher than in seabirds. The compound 2-(2H-benzotriazol-2-yl)-4-(1,1,3,3-tetramethylbutyl)phenol (UV329) or its isomer 2-(2H-benzotriazol-2-yl)-4-(tert-butyl)-6-(sec-butyl) phenol (UV350) was the predominant BZT-UVs in seals, with the concentrations of ΣBZT-UVs between <method quantification limits and 1.66 × 104 pg g-1 (ww) (median: 2.36 × 103 pg g-1, ww). This is the first report of the different distribution patterns of SDPAs and BZT-UVs in wildlife from Canadian Arctic sites.

Lu Z ，De Silva AO ，Provencher JF ，Mallory ML ，Kirk JL ，Houde M ，Stewart C ，Braune BM ，Avery-Gomm S ，Muir DCG ... -  《-》

Ultraviolet absorbents and industrial antioxidants in seabirds, mammals, and fish from the Canadian Arctic.

Ultraviolet (UV) absorbents and industrial antioxidants are two groups of plastic-derived contaminants of emerging environmental concern. However, their distribution and fate are poorly understood in Arctic wildlife. In the present study, 16 UV absorbents (10 benzotriazole UV stabilizers (BZT-UVs) and 6 organic UV filters (UVFs)) and 7 industrial antioxidants (6 aromatic secondary amines (Ar-SAs) and 2,6-di-tert-butylphenol (26DTBP)) were analyzed in the livers of thick-billed murre (Uria lomvia; n = 28), northern fulmar (Fulmarus glacialis; n = 4), black guillemot (Cepphus grylle; n = 11), polar bear (Ursus maritimus; n = 18), beluga whale (Delphinapterus leucas; n = 10), landlocked (n = 25) and sea-run (n = 10) Arctic char (Salvelinus alpinus) from the Canadian Arctic collected between 2017 and 2021. Compared to industrial antioxidants (median range: ΣAr-SAs: not calculated due to detection frequency < 30 % (NA)-4.06 ng/g, wet weight (ww); 26DTBP: NA-1.91 ng/g ww), UV absorbents (median range: ΣBZT-UVs: NA-8.71 ng/g ww; ΣUVFs: NA-48.3 ng/g ww) generally showed greater concentrations in the liver of these species. Seabirds accumulated higher levels of these contaminants (median range: ΣBZT-UVs: 3.38-8.71 ng/g ww; ΣUVFs: NA-48.3 ng/g ww; ΣAr-SAs: 0.07-4.06 ng/g ww; 26DTBP: NA-1.14 ng/g ww)) than the other groups (median range: ΣBZT-UVs: NA-1.31 ng/g ww; ΣUVFs: NA-4.22 ng/g ww; ΣAr-SAs: NA; 26DTBP: NA-1.91 ng/g ww), suggesting that seabirds may be useful indicator species for future long-term monitoring. The livers of Arctic char in the Canadian Arctic generally contain lower levels of these contaminants than those of freshwater fish in temperate regions. Spatial variations were found in the liver of black guillemots, Hudson Bay polar bears, and landlocked char for some target contaminants, indicating differences in the levels of these contaminants in their surrounding environment or diet. Consumption of liver tissues from these species may expose humans to varying levels of UV absorbents and industrial antioxidants. This study establishes a baseline for future research of the spatial and temporal trends of these contaminants in Arctic species. It provides the basis for elucidating the fate of these contaminants and assessing their adverse effects at environmental-relevant concentrations in the Arctic. Factors influencing the accumulation patterns of these contaminants in Arctic biota and their potential health risks require further investigation.

Granados-Galvan IA ，Provencher JF ，Mallory ML ，De Silva A ，Muir DCG ，Kirk JL ，Wang X ，Letcher RJ ，Loseto LL ，Hamilton BM ，Lu Z ... -  《-》

Occurrence and fate of substituted diphenylamine antioxidants and benzotriazole UV stabilizers in various Canadian wastewater treatment processes.

Substituted diphenylamine antioxidants (SDPAs) and benzotriazole UV stabilizers (BZT-UVs) are additives used in industrial and consumer products to prevent degradation or color change of materials, but their environmental fate and disposition are not well characterized. In this study, SDPAs and BZT-UVs were analyzed in 68 liquid and 39 solid samples collected from 9 wastewater treatment plants (WWTPs) in Canada to investigate the occurrence and fate of these contaminants. The median concentrations of ΣSDPAs and ΣBZT-UVs was 483 and 76.2 ng L-1 in influent, 28.4 and 4.84 ng L-1 in effluent, and 2750 and 457 ng g-1 in biosolids (dry weight), respectively. Dinonyl-diphenylamine (C9C9) was the predominant congener of SDPAs in all matrices (>40%). For target BZT-UVs, the major components were 2-(2H-benzotriazol-2-yl)-4,6-bis(1-methyl-1-phenylethyl)phenol (UV234) and 2-(2H-benzotriazol-2-yl)-4,6-di-tert-pentylphenol (UV328). SDPAs and BZT-UVs were effectively removed (>90%) from the liquid stream in most WWTPs mainly through sludge sorption and separation, but biotransformation, UV treatment and filtration may also contribute to removal of some contaminants in advanced treatment plants. In contrast, the removal efficiency of target contaminants using chemically assisted primary treatment was low, likely due to the short hydraulic retention time of this site. Our results suggest that wastewater effluent is a vector of SDPAs and BZT-UVs to the aquatic environment. The results also highlight the high concentrations of SDPAs and BZT-UVs associated with the solid stream in WWTPs, which could affect the beneficial use of biosolids (e.g., compost or land applications).

Lu Z ，Smyth SA ，Peart TE ，De Silva AO ... -  《-》

Substituted diphenylamine antioxidants and benzotriazole UV stabilizers in blood plasma of fish, turtles, birds and dolphins from North America.

Substituted diphenylamine antioxidants (SDPAs) and benzotriazole UV stabilizers (BZT-UVs) are additives used in industrial and commercial applications to prevent degradation by oxidation and are contaminants of emerging environmental concern. Little is known about the fate of these contaminants in wildlife, particularly in reptiles, birds and marine mammals. Nine SDPAs and six BZT-UVs were measured in blood plasma of seven fish species, snapping turtles (Chelydra serpentina), double-crested cormorants (Phalacrocorax auritus), and bottlenose dolphins (Tursiops truncatus) from various locations in North America. Plasma SDPAs were more frequently (90-100%) detected and with higher concentrations (median: 25-270 pg g-1, wet weight (ww)) in organisms from urban areas than rural locations (median: <method limits of quantification -136 pg g-1). The concentrations of most SDPAs generally followed the order of fish ≥ snapping turtles > double-crested cormorants > bottlenose dolphins. Of the three quantifiable BZT-UVs, 2-(2H-benzotriazol-2-yl)-4,6-di-tert-pentylphenol (UV328) showed higher detection frequency in most species of fish, bird and turtle (range of 0-67%), indicating the widespread distribution of UV328 in the aquatic environment of lower Great Lakes region.

Lu Z ，De Silva AO ，Zhou W ，Tetreault GR ，de Solla SR ，Fair PA ，Houde M ，Bossart G ，Muir DCG ... -  《-》

Distribution and trophodynamics of substituted diphenylamine antioxidants and benzotriazole UV stabilizers in a freshwater ecosystem and the adjacent riparian environment.

Lu Z ，De Silva AO ，Spencer C ，Tetreault GR ，de Solla SR ，Muir DCG ... -  《-》