Long noncoding RNA LINC-PINT is inhibited in gastric cancer and predicts poor survival.

Long noncoding RNA (lncRNA) LINC-PINT expression is inhibited in many types of cancer cells, suggesting its role as a tumor suppressor. However, the functionality of LINC-PINT in gastric cancer and the clinical values are unknown. In the present study, we found that lncRNA LINC-PINT was downregulated, while microRNA-21 (miR-21) was upregulated in tumor tissues than in adjacent healthy tissues of gastric cancer patients. A significant and inverse correlation between expression levels of lncRNA LINC-PINT and miR-21 was found in both tumor tissues and adjacent healthy tissues. The low expression level of LINC-PINT and high expression level of the miR-21 tumor were correlated with poor prognosis. LINC-PINT overexpression casued miR-21 inhibition in cells of human gastric cancer cell lines, while miR-21 overexpression did not alter LINC-PINT expression. LINC-PINT overexpression led to inhibited, while miR-21 overexpression led to promoted proliferation, migration, and invasion of gastric cancer cells. Effects of LINC-PINT overexpression on cellular behaviors of gastric cancer cells were attenuated by miR-21 overexpression. Therefore, LINC-PINT may participate in gastric cancer through the crosstalk with miR-21.

Feng H ，Zhang J ，Shi Y ，Wang L ，Zhang C ，Wu L ... -  《-》

LncRNA LINC-PINT Inhibits Cancer Cell Proliferation, Invasion, and Migration in Osteosarcoma by Downregulating miRNA-21.

Liu W 《-》

LINC-PINT suppresses tumour cell proliferation, migration and invasion through targeting miR-374a-5p in ovarian cancer.

LncRNA LINC-PINT acts as an important regulator in the development of many cancers. The current study aimed to explore the role of LINC-PINT in the progression of ovarian cancer (OC). LINC-PINT expression level in different FIGO stages of OC and its adjacent tissues, normal HOSE and OC cell lines (A2780, SKOV3, OVCAR3 and HO-8910) was determined by qRT-PCR. Survival analysis on LINC-PINT and OC patients was conducted by Kaplan-Meier. CCK-8, flow cytometry, wound-healing, Transwell assays and western blot were performed to detect the effects of LINC-PINT on proliferation, apoptosis, migration, invasion and EMT process in OC cells. Target gene of LINC-PINT was predicted by Starbase and verified by dual-luciferase reporter assay. The expression of miR-374a-5p in normal and OC tissues, LINC-PINT- or siLINC-PINT-modified OC cells was determined. Moreover, rescue assay was carried out to confirm whether LINC-PINT contributes to the development of OC cells through targeting miR-374a-5p. Low expression of LINC-PINT was observed in OC tissues and cells, noticeably, LINC-PINT expression was even lower in OC tissues with higher FIGO stage. Increased LINC-PINT expression significantly inhibited cell proliferation, promoted apoptosis and suppressed migration, invasion and EMT process, while silencing of LINC-PINT caused the opposite results. Moreover, LINC-PINT sponged miR-374a-5p and overexpressed miR-374a-5p attenuated the effect of up-regulated LINC-PINT on cell viability, migration, invasion and apoptosis. LINC-PINT acts as a tumour suppressor, as it could inhibit cell proliferation, migration, invasion and EMT process, and promote cell apoptosis through down-regulating miR-374a-5p. SIGNIFICANCE OF THE STUDY: Ovarian cancer (OC), which is a frequently diagnosed tumour in female reproductive organs, has a high incidence rate behind cervical cancer and endometrial cancer. LncRNA LINC-PINT acts as an important regulator in the development of many cancers. The current study aimed to explore the role of LINC-PINT in the progression of ovarian cancer (OC) and found that LINC-PINT inhibited cell proliferation, migration invasion and EMT process of OC cell via regulating miR-374a-5p; it might be a potential target for OC treatment.

Hao T ，Huang S ，Han F 《-》

LncRNA LINC-PINT increases SOCS1 expression by sponging miR-155-5p to inhibit the activation of ERK signaling pathway in rheumatoid arthritis synovial fibroblasts induced by TNF-α.

Rheumatoid arthritis (RA) is a systemic and chronic autoimmune disease associated with altered gene expression in synovial tissue. Long intergenic non-protein encoding long-chain RNA p53-induced transcript (LncRNA LINC-PINT) has been reported to be involved in multiple physiological and pathological processes. However, the role of lncRNA LINC-PINT in RA is rarely mentioned. To investigate the mechanism of LINC-PINT in RA, we constructed a RA model using TNF-α-induced method to explore the downstream effector and signaling pathway. We found that LINC-PINT was downregulated in RA tissues and TNF-α stimulated RA cells. And overexpression of LINC-PINT could inhibit cell proliferation and invasion induced by TNF-α through downregulating the levels of IL-1β and MMPs and inhibiting the activation of ERK pathway. Using bioinformatics analysis and RNA Binding Protein Immunoprecipitation (RIP) assay, we verified that LINC-PINT directly interacted with miR-155-5p, and miR-155-5p could regulate the expression of SOCS1. Whereas, downregulation of miR-155-5p inhibited cell growth and invasion. Overexpression of miR-155-5p could reverse the inhibitory effect of LINC-PINT induced by TNF-α. Furthermore, silencing SOCS1 promoted cell proliferation and invasion, upregulated the expression of IL-1β and MMPs, and activated ERK pathway, while overexpression of LINC-PINT could reverse the control of knocking down SOCS1. In conclusion, we revealed that LINC-PINT suppressed TNF-α induced cell proliferation and invasion which might be induced through downregulating miR-155-5p, influencing the expression of SOCS1, IL-1β and MMPs and inactivating ERK signaling pathway.

Wang J ，Zhao Q 《-》

Long noncoding RNA LINC-PINT inhibits non-small cell lung cancer progression through sponging miR-218-5p/PDCD4.

Zhang L ，Hu J ，Li J ，Yang Q ，Hao M ，Bu L ... -  《-》