Global regulatory function of the low oxygen-induced transcriptional regulator LoiA in Salmonella Typhimurium revealed by RNA sequencing.

Salmonella enterica serovar Typhimurium (S. Typhimurium) is a major intestinal pathogen that can infect both humans and a variety of animals. LoiA, a novel virulence-regulating protein encoded in Salmonella pathogenicity island (SPI)-14, has been shown to be induced under low oxygen conditions and contribute to S. Typhimurium invasion into intestinal epithetical cells by activating the SPI-1 invasion genes. However, the global regulatory network of LoiA remains unknown. Here, we used high-throughput RNA sequencing (RNA-seq) technology to investigate the regulatory function of LoiA in S. Typhimurium under low oxygen conditions. A total of 1250 genes were differentially expressed between the loiA mutant and the wild-type strain; 413 genes were up-regulated and 837 were down-regulated. SPI-1 gene expression was down-regulated in the loiA mutant, consistent with previous results. SPI-2 gene expression was not affected by deletion of loiA; the expression of most genes involved in flagellar basal body and hook biosynthesis was up-regulated in the loiA mutant, while the expression of genes associated with flagellin, motility, and chemotaxis was down-regulated; the expression of lon, encoding an ATP-dependent protease, was up-regulated in the mutant. This study indicates that LoiA regulates a variety of virulence-associated genes in S. Typhimurium. The negative regulation of Lon protease by LoiA indicates that LoiA can regulates several virulence-associated genes in S. Typhimurium via the Lon protease.

Li H ，Li X ，Lv R ，Jiang X ，Cao H ，Du Y ，Jiang L ，Liu B ... -  《-》

LoiA directly represses lon gene expression to activate the expression of Salmonella pathogenicity island-1 genes.

Salmonella enterica serovar Typhimurium uses virulence factors encoded by Salmonella pathogenicity island 1 (SPI-1) to invade the intestinal epithelium. The low oxygen-induced transcriptional regulator LoiA is established to be a positive regulator of SPI-1 genes and can also repress the expression of the ATP-dependent Lon protease, a negative regulator of SPI-1 genes. Whether the repression of lon by LoiA is associated with its regulation of SPI-1 genes, as well as the regulatory mechanism involved, is unknown. In this study, we showed that LoiA directly represses the expression of the lon gene during invasion by binding to the promoter region of lon. The activation of S. Typhimurium SPI-1 gene expression by LoiA is associated with its repression of lon. Moreover, through invasion and mouse infection assays, we found that the repression of lon by LoiA contributes to S. Typhimurium invasion of intestinal epithelial cells and virulence in mice. However, LoiA does not influence lon expression during S. Typhimurium growth in macrophages, which may be associated with the low expression level of loiA in macrophages. Collectively, these findings establish the direct regulation of lon by LoiA and describe a novel mechanism by which LoiA regulates SPI-1 gene expression.

Jiang L ，Li X ，Lv R ，Feng L ... -  《-》

Signal transduction pathway mediated by the novel regulator LoiA for low oxygen tension induced Salmonella Typhimurium invasion.

Jiang L ，Feng L ，Yang B ，Zhang W ，Wang P ，Jiang X ，Wang L ... -  《-》

Transcriptome analysis of virulence gene regulation by the ATP-dependent Lon protease in Salmonella Typhimurium.

Song X ，Zhang H ，Ma S ，Song Y ，Lv R ，Liu X ，Yang B ，Huang D ，Liu B ，Jiang L ... -  《-》

FNR is a global regulator of virulence and anaerobic metabolism in Salmonella enterica serovar Typhimurium (ATCC 14028s).

Fink RC ，Evans MR ，Porwollik S ，Vazquez-Torres A ，Jones-Carson J ，Troxell B ，Libby SJ ，McClelland M ，Hassan HM ... -  《-》